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Anti-HLA G抗体[MEM-G/11] (ab4570)

Submit a review Q&A (2)References (3)

Key features and details

  • Mouse monoclonal [MEM-G/11] to HLA G
  • Suitable for: ELISA, IP, Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

FITC

概述

  • 产品名称

    Anti-HLA G抗体[MEM-G/11]
    参阅全部 HLA G 一抗

  • 描述

    小鼠单克隆抗体[MEM-G/11] to HLA G

  • 宿主

    Mouse

  • 经测试应用

    适用于: ELISA, IP, Flow Cyt, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length protein (Human) refolded with beta 2 microglobulin and peptide.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    MEM-G/11

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4570于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

The antibody MEM-G/11 does not cross-block standard MEM-G/9 antibody. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. PubMed: 17549736
说明
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

The antibody MEM-G/11 does not cross-block standard MEM-G/9 antibody. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. PubMed: 17549736

靶标

  • 功能

    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.

  • 组织特异性

    Expressed in trophoblasts.

  • 序列相似性

    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • B2 microglobulin antibody
      • DADB-15K14.8 antibody
      • HLA 6.0 antibody
      • HLA class I histocompatibility antigen alpha chain G antibody
      • HLA class I histocompatibility antigen, alpha chain G antibody
      • HLA class I molecule antibody
      • HLA G antibody
      • HLA G antigen antibody
      • HLA G histocompatibility antigen class I G antibody
      • HLA G3 antibody
      • HLA-G antibody
      • HLA-G histocompatibility antigen, class I antibody
      • HLA60 antibody
      • HLAG antibody
      • HLAG_HUMAN antibody
      • Major histocompatibility complex class I G antibody
      • MHC class I antigen antibody
      • MHC class I antigen G antibody
      • MHC G antibody
      • T-cell A locus antibody
      • TCA antibody
      see all

    文献 (3)

    发表研究结果有使用 ab4570？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab4570 被引用在 3 文献中.

    • Apps R  et al. A homodimeric complex of HLA-G on normal trophoblast cells modulates antigen-presenting cells via LILRB1. Eur J Immunol 37:1924-37 (2007). WB . PubMed: 17549736
    • Menier C  et al. Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol 64:315-26 (2003). PubMed: 12590976
    • Boyson JE  et al. Disulfide bond-mediated dimerization of HLA-G on the cell surface. Proc Natl Acad Sci U S A 99:16180-5 (2002). PubMed: 12454284

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    One of our customers wishes to simultaneously bind 2 antibodies to magnetic beads for IP. I do not see why there should be any problem with this procedure.
    Do you foresee any cross reactivity or specicific problems with the binding both these antibodies to magnetic beads?
    ab42454, ab4570.

    Read More
    Answer




    I don't expect any problems with the ab4570 as this one works fine in IP as well, however, I am not sure about the ab42454.

    Nonetheless, I am not too concerned either: ab42454 is not tested in IP, but it works in ELISA, which is a good indicator that it will react with the native protein.

    Read More

    Question

    Does this antibody recognize any of the isotypes (G1, G2, G3, G4)? For instance, clone MEM-G9 specificities was demonstrated with cells transfected with either full-length HLA-G and HLA-G1 complementary DNA.

    Read More
    Answer

    Antibodies MEM-G/9 and MEM-G/11 recognize HLA-G1 (full length) but not truncated isoforms (G2, G3, G4) on the cell surface. Both react with HLA-G5 (G6 was not rigorously tested), i.e. the soluble isoform. MEM-G/11 reacts with a different epitope than MEM-G/9, this could be useful in double staining (MEM-G/11 is available as FITC and MEM-G/9 as PE conjugate) of cell surface HLA-G1. In a soluble form (actively secreted G5 or shedded G1), it was not demonstrated that both mAbs can be used as pair in sandwich ELISA.

    Read More

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