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Anti-HLA G抗体[MEM-G/1] (ab7759)

Reviews (3)Q&A (10)References (25)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
  • Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
  • Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)

Key features and details

  • Mouse monoclonal [MEM-G/1] to HLA G
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Biotin

概述

  • 产品名称

    Anti-HLA G抗体[MEM-G/1]
    参阅全部 HLA G 一抗

  • 描述

    小鼠单克隆抗体[MEM-G/1] to HLA G

  • 宿主

    Mouse

  • 特异性

    The antibody reacts with denaturated HLA-G heavy chain.

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length denatured heavy chain (Human).

  • 阳性对照

    • IHC-P: First trimester placenta and pulmonary diseases. WB: ab29745; HeLa and JEG-3 cell lysates.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    Purified from TCS. Purity >95% by SDS-PAGE.

  • 克隆

    单克隆

  • 克隆编号

    MEM-G/1

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7759于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
1/60 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB (1)
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 38.2 kDa.
说明
IHC-P
1/60 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 38.2 kDa.

靶标

  • 功能

    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.

  • 组织特异性

    Expressed in trophoblasts.

  • 序列相似性

    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • B2 microglobulin antibody
      • DADB-15K14.8 antibody
      • HLA 6.0 antibody
      • HLA class I histocompatibility antigen alpha chain G antibody
      • HLA class I histocompatibility antigen, alpha chain G antibody
      • HLA class I molecule antibody
      • HLA G antibody
      • HLA G antigen antibody
      • HLA G histocompatibility antigen class I G antibody
      • HLA G3 antibody
      • HLA-G antibody
      • HLA-G histocompatibility antigen, class I antibody
      • HLA60 antibody
      • HLAG antibody
      • HLAG_HUMAN antibody
      • Major histocompatibility complex class I G antibody
      • MHC class I antigen antibody
      • MHC class I antigen G antibody
      • MHC G antibody
      • T-cell A locus antibody
      • TCA antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)

      Immunohistochemistry staining with ab7759 of first trimester placenta (paraffin-embedded sections).

    • Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)
      Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)
      All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 10 µg/ml

      Lane 1 : Human placenta tissue lysate - total protein (ab29745)
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 38.2 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?
      Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
      The antibody ab7759 stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.
    • Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)
      Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)
      All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 2 µg/ml

      Lane 1 : JEG-3 cell line under reducing conditions.
      Lane 2 : LNCap cell line under reducing conditions.
      Lane 3 : JEG-3 cell line under non-reducing conditions.
      Lane 4 : LNCap cell line under non-reducing conditions.

      Secondary
      All lanes : IRDye800-conjugated anti-mouse secondary antibody

      Predicted band size: 38.2 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?



      Western blotting analysis of human HLA-G using ab7759 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions.

      Nitrocellulose membrane was probed with 2 µg/ml of ab7759 followed by IRDye800-conjugated anti-mouse secondary antibody. 

    文献 (25)

    发表研究结果有使用 ab7759？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab7759 被引用在 25 文献中.

    • Wang LJ  et al. Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation. Nat Commun 13:1626 (2022). PubMed: 35338152
    • Bi Y  et al. Cell fate roadmap of human primed-to-naive transition reveals preimplantation cell lineage signatures. Nat Commun 13:3147 (2022). PubMed: 35672317
    • Tan JP  et al. Establishment of human induced trophoblast stem cells via reprogramming of fibroblasts. Nat Protoc 17:2739-2759 (2022). PubMed: 36241724
    • Qiu W  et al. RNA Demethylase FTO Mediated RNA m6A Modification Is Involved in Maintaining Maternal-Fetal Interface in Spontaneous Abortion. Front Cell Dev Biol 9:617172 (2021). PubMed: 34350169
    • Shahbazi MN  et al. Developmental potential of aneuploid human embryos cultured beyond implantation. Nat Commun 11:3987 (2020). PubMed: 32778678
    View all Publications for this product

    客户评价及客户问答

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    Western blot abreview for Anti-HLA G antibody [MEM-G/1]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (JEG-3)
    Loading amount
    30 µg
    Specification
    JEG-3
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    提交于 Jun 01 2010

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