Anti-HLA G抗体[G233] (ab52454)
![Flow Cytometry - Anti-HLA G antibody [G233] (ab52454)](https://www.abcam.cn/ps/products/52/ab52454/Images/ab52454-522217-anti-hla-g-antibody-g233-flow-cytometry-human.jpg "Flow Cytometry - Anti-HLA G antibody [G233] (ab52454)")
Key features and details
- Mouse monoclonal [G233] to HLA G
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-HLA G抗体[G233]
参阅全部 HLA G 一抗 -
描述
小鼠单克隆抗体[G233] to HLA G -
宿主
Mouse -
特异性
ab52454 recognises several isoforms of HLA-G expressed in all populations of extravillous trophoblast (cell columns, interstitial trophoblast, endovascular trophoblast, placental bed giant cells). It does not cross-react with any other MHC Class I antigens (HLA-A, -B, -C, -E, -F). -
经测试应用
适用于: Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human HLA G. Murine L cells transfected with both human beta2-microglobulin and HLA-G
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阳性对照
- Jeg3 cell line.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
G233 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52454于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
|
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| ICC/IF | (1) |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
Flow Cyt
Use a concentration of 1 - 4 µg/ml. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
组织特异性
Expressed in trophoblasts. -
序列相似性
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3135 Human
- Omim: 142871 Human
- SwissProt: P17693 Human
- Unigene: 512152 Human
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别名
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
图片
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Flow Cytometry - Anti-HLA G antibody [G233] (ab52454)
Flow cytometry analysis (surface staining) of HLA-G in HLA-G transfectants using ab52454 purified / GAM-APC at 4 μg/ml. Blue - K562 cells. Red - HLA-G transfected LCL cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-HLA G antibody [G233] (ab52454)](https://www.abcam.cn/ps/products/52/ab52454/Images/ab52454-64562-anti-hla-g-antibody-g233-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HLA G antibody [G233] (ab52454)ICC/IF image of ab52454 stained Jeg3 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52454, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab52454 被引用在 8 文献中.
- Yang L et al. Trophoblast organoids with physiological polarity model placental structure and function. J Cell Sci 137:N/A (2024). PubMed: 37676312
- Lv B et al. Single-cell RNA sequencing reveals regulatory mechanism for trophoblast cell-fate divergence in human peri-implantation conceptuses. PLoS Biol 17:e3000187 (2019). PubMed: 31596842
- Cho S et al. The C-terminal amino acid of the MHC-I heavy chain is critical for binding to Derlin-1 in human cytomegalovirus US11-induced MHC-I degradation. PLoS One 8:e72356 (2013). Human . PubMed: 23951315
- Gonzalez A et al. Identification of circulating nonclassic human leukocyte antigen G (HLA-G)-like molecules in exudates. Clin Chem 57:1013-22 (2011). PubMed: 21527645
- Imudia AN et al. Retrieval of trophoblast cells from the cervical canal for prediction of abnormal pregnancy: a pilot study. Hum Reprod 24:2086-92 (2009). PubMed: 19497946
- Bulmer JN et al. HLA-G positive trophoblastic cells in transcervical samples and their isolation and analysis by laser microdissection and QF-PCR. Prenat Diagn 23:34-9 (2003). PubMed: 12533810
- Frumento G et al. Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment. Tissue Antigens 56:30-7 (2000). PubMed: 10958353
- Rajagopalan S & Long EO A human histocompatibility leukocyte antigen (HLA)-G-specific receptor expressed on all natural killer cells. J Exp Med 189:1093-100 (1999). PubMed: 10190900