重组Anti-HLA G抗体[EPR23298-97] (ab283260)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23298-97] to HLA G
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-HLA G抗体[EPR23298-97]
参阅全部 HLA G 一抗 -
描述
兔单克隆抗体[EPR23298-97] to HLA G -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: JEG-3 whole cell lysate; Human placenta tissue lysate. IHC-P: Human placenta, Human breast carcinoma and Human cardiac muscle tissues. ICC/IF: JEG-3 cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23298-97 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab283260于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/20000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
1/1000. Predicted molecular weight: 38 kDa.
|
|
ICC/IF |
1/100.
|
说明 |
---|
IHC-P
1/20000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 38 kDa. |
ICC/IF
1/100. |
靶标
-
功能
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
组织特异性
Expressed in trophoblasts. -
序列相似性
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 3135 Human
- Omim: 142871 Human
- SwissProt: P17693 Human
- Unigene: 512152 Human
-
别名
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
图片
-
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling HLA G with ab283260 at 1/20000 (0.026 µg/mL) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on extravillous trophoblasts (EVTs) in human placenta (PMID:18538632, PMID:29249463, PMID:29249436). The section was incubated with ab283260 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
All lanes : Anti-HLA G antibody [EPR23298-97] (ab283260) at 1/1000 dilution
Lane 1 : JEG-3 (human placenta choriocarcinoma epithelial cell) whole cell lysate
Lane 2 : HuT-78 (human sezary syndrome cutaneous T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: HuT-78 (PMID:2249951).
Exposure time: 48 seconds.
-
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized JEG-3 cells labelling HLA G with ab283260 at 1/100 (5.18 µg/mL) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/mL) dilution (Green). Confocal image showing cytoplasmic and membranous staining in JEG-3 cell line. Negative control: HuT-78 (PMID: 2249951). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/mL) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
-
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling HLA G with ab283260 at 1/20000 (0.026 µg/mL) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human breast carcinoma. The section was incubated with ab283260 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
All lanes : Anti-HLA G antibody [EPR23298-97] (ab283260) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Human heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: heart (PMID:22438923).
Exposure time: 26 seconds.
-
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling HLA G with ab283260 at 1/20000 (0.026 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on human cardiac muscle (PMID:22438923). The section was incubated with ab283260 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (2)
ab283260 被引用在 2 文献中.
- Shi JW et al. An IGF1-expressing endometrial stromal cell population is associated with human decidualization. BMC Biol 20:276 (2022). PubMed: 36482461
- Zhong Q et al. Structural insights into the covalent regulation of PAPP-A activity by proMBP and STC2. Cell Discov 8:137 (2022). PubMed: 36550107