Anti-HLA G抗体[4H84] (ab52455)
![Western blot - Anti-HLA G antibody [4H84] (ab52455)](https://www.abcam.cn/ps/products/52/ab52455/Images/ab52455-481939-antihla-g-antibody-4h84-western-blot.JPG "Western blot - Anti-HLA G antibody [4H84] (ab52455)")
Key features and details
- Mouse monoclonal [4H84] to HLA G
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-HLA G抗体[4H84]
参阅全部 HLA G 一抗 -
描述
小鼠单克隆抗体[4H84] to HLA G -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human HLA G aa 61-83.
Sequence:DSDSACPRMEPRAPWVEQEGPEY
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阳性对照
- WB: JEG-3 cell line.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
4H84 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52455于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
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| IHC-P | (2) |
Use a concentration of 10 µg/ml.
|
| WB | (2) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 38 kDa.
|
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 38 kDa. |
靶标
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功能
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
组织特异性
Expressed in trophoblasts. -
序列相似性
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3135 Human
- Omim: 142871 Human
- SwissProt: P17693 Human
- Unigene: 512152 Human
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别名
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
图片
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Western blot - Anti-HLA G antibody [4H84] (ab52455)All lanes : Anti-HLA G antibody [4H84] (ab52455) at 2 µg/ml
Lane 1 : JEG-3 cell line under reducing conditions.
Lane 2 : LNCaP cell line under reducing conditions.
Lane 3 : JEG-3 cell line under non-reducing conditions.
Lane 4 : LNCaP cell line under non-reducing conditions.
Secondary
All lanes : IRDye800-conjugated anti-mouse
Predicted band size: 38 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Western blotting analysis of human HLA-G using ab52455 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions.
Nitrocellulose membrane was probed with 2 µg/ml of ab52455 followed by IRDye800-conjugated anti-mouse secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (36)
ab52455 被引用在 36 文献中.
- Karakis V et al. Laminin switches terminal differentiation fate of human trophoblast stem cells under chemically defined culture conditions. J Biol Chem 299:104650 (2023). PubMed: 36972789
- Horii M et al. Histopathologic and Transcriptomic Profiling Identifies Novel Trophoblast Defects in Patients With Preeclampsia and Maternal Vascular Malperfusion. Mod Pathol 36:100035 (2023). PubMed: 36853788
- Barreto-Arce LJ et al. Protection against brain injury after ischemic stroke by intravenous human amnion epithelial cells in combination with tissue plasminogen activator. Front Neurosci 17:1157236 (2023). PubMed: 37397458
- Castel G & David L Induction of human trophoblast stem cells. Nat Protoc 17:2760-2783 (2022). PubMed: 36241723
- Plianchaisuk A et al. Origination of LTR Retroelement-Derived NYNRIN Coincides with Therian Placental Emergence. Mol Biol Evol 39:N/A (2022). PubMed: 35959649