重组Anti-HLA F抗体[EPR6803] (ab126624)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)](https://www.abcam.cn/ps/products/126/ab126624/Images/ab126624-140621-anti-hla-f-antibody-epr6803-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6803] to HLA F
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-HLA F抗体[EPR6803]
参阅全部 HLA F 一抗 -
描述
兔单克隆抗体[EPR6803] to HLA F -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human tonsil tissue; Human tonsil, HT1376 and JAR cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 2.52 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6803 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126624于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
|
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| IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Involved in the presentation of foreign antigens to the immune system. -
序列相似性
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3134 Human
- Omim: 143110 Human
- SwissProt: P30511 Human
- Unigene: 519972 Human
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别名
- CDA12 antibody
- HLA 5.4 antibody
- HLA class I histocompatibility antigen, alpha chain F antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)
ab126624, at a 1/250 dilution, staining HLA F in paraffin-embedded Human tonsil tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-HLA F antibody [EPR6803] (ab126624)](https://www.abcam.cn/ps/products/126/ab126624/Images/ab126624-140620-anti-hla-f-antibody-epr6803-western-blot.jpg)
Western blot - Anti-HLA F antibody [EPR6803] (ab126624)All lanes : Anti-HLA F antibody [EPR6803] (ab126624) at 1/1000 dilution
Lane 1 : HT1376 cell lysate
Lane 2 : Human tonsil lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa -
![OI-RD Scanning - Anti-HLA F antibody [EPR6803] (ab126624)](https://www.abcam.cn/ps/products/126/ab126624/Images/ab126624-140619-anti-hla-f-antibody-epr6803.jpg)
OI-RD Scanning - Anti-HLA F antibody [EPR6803] (ab126624) -
![Anti-HLA F antibody [EPR6803] (ab126624)](https://www.abcam.cn/ps/products/126/ab126624/Images/ab126624-4-benefits-of-recombinant-antibodies.png)
Anti-HLA F antibody [EPR6803] (ab126624)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab126624 被引用在 2 文献中.
- Peng W et al. Silencing of circCRIM1 Drives IGF2BP1-Mediated NSCLC Immune Evasion. Cells 12:N/A (2023). PubMed: 36672208
- Wu B et al. High HLA-F Expression Is a Poor Prognosis Factor in Patients with Nasopharyngeal Carcinoma. Anal Cell Pathol (Amst) 2018:7691704 (2018). PubMed: 30510890