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Anti-HLA E抗体[MEM-E/07] (ab11820)

Reviews (3)Q&A (1)References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA E antibody [MEM-E/07] (ab11820)
  • Flow Cytometry - Anti-HLA E antibody [MEM-E/07] (ab11820)

Key features and details

  • Mouse monoclonal [MEM-E/07] to HLA E
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-HLA E抗体[MEM-E/07]
    参阅全部 HLA E 一抗

  • 描述

    小鼠单克隆抗体[MEM-E/07] to HLA E

  • 宿主

    Mouse

  • 特异性

    The antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules: HLA B7 (strongly), HLA B8 (moderately), HLA B27, HLA-B44 (weakly).

  • 经测试应用

    适用于: Flow Cyt, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length protein corresponding to HLA E. The epitope is extracellular. The antibody reacts only with native epitope, not denatured.
    Database link: P13747

  • 阳性对照

    • IHC-P: Human spleen tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    Purified from hybridoma culture supernatant by protein A-affinity chromatography.

  • 克隆

    单克隆

  • 克隆编号

    MEM-E/07

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab11820于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (1)
Use a concentration of 1 - 4 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.
说明
Flow Cyt
Use a concentration of 1 - 4 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.

靶标

  • 相关性

    HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

  • 细胞定位

    Membrane; Single-pass type I membrane protein

  • 数据库链接

    • 别名

      • HLA class I histocompatibility antigen E alpha chain antibody
      • EA1.2 antibody
      • EA2.1 antibody
      • HLA 6.2 antibody
      • HLA class I histocompatibility antigen alpha chain E antibody
      • HLA class I histocompatibility antigen E alpha chain precursor antibody
      • HLA class I histocompatibility antigen E alpha chain precursor antibody
      • HLA6.2 antibody
      • HLAE antibody
      • Lymphocyte antigen antibody
      • Major histocompatibility complex class I E antibody
      • MHC antibody
      • MHC class I antigen E antibody
      • MHC HLA E alpha 1 antibody
      • MHC HLA E alpha 2.1 antibody
      • QA1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA E antibody [MEM-E/07] (ab11820)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA E antibody [MEM-E/07] (ab11820)

      Immunohistochemistry parafin embedded sections staining of huam spleen tissue using ab11820.

    • Flow Cytometry - Anti-HLA E antibody [MEM-E/07] (ab11820)
      Flow Cytometry - Anti-HLA E antibody [MEM-E/07] (ab11820)

      Flow cytometry analysis showing separation of HLA-E transfected LCL cells stained using ab11820 (concentration in sample 2 μg/ml, red-filled) from HLA-E transfected LCL cells stained using mouse IgG1 isotype control purified antibody (concentration in sample 2 μg/ml, same as HLA-E purified antibody concentration, black-dashed).

    文献 (8)

    发表研究结果有使用 ab11820？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab11820 被引用在 8 文献中.

    • Samiec M  et al. The Relative Abundances of Human Leukocyte Antigen-E, α-Galactosidase A and α-Gal Antigenic Determinants Are Biased by Trichostatin A-Dependent Epigenetic Transformation of Triple-Transgenic Pig-Derived Dermal Fibroblast Cells. Int J Mol Sci 23:N/A (2022). PubMed: 36142211
    • Sasaki T  et al. Microenvironmental stresses induce HLA-E/Qa-1 surface expression and thereby reduce CD8(+) T-cell recognition of stressed cells. Eur J Immunol 46:929-40 (2016). PubMed: 26711740
    • Shwetank  et al. Inhibition of ERK and proliferation in NK cell lines by soluble HLA-E released from Japanese encephalitis virus infected cells. Immunol Lett 162:94-100 (2014). PubMed: 25086398
    • Shwetank  et al. Infection of human endothelial cells by Japanese encephalitis virus: increased expression and release of soluble HLA-E. PLoS One 8:e79197 (2013). Human . PubMed: 24236107
    • Jagessar SA  et al. Unravelling the T-cell-mediated autoimmune attack on CNS myelin in a new primate EAE model induced with MOG34-56 peptide in incomplete adjuvant. Eur J Immunol 42:217-27 (2012). PubMed: 21928277
    • Jørgensen PB  et al. Epstein-Barr virus Peptide Presented by HLA-E is Predominantly Recognized by CD8(bright) Cells in multiple Sclerosis Patients. PLoS One 7:e46120 (2012). Flow Cyt . PubMed: 23049954
    • Park B  et al. The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradation. J Exp Med 207:2033-41 (2010). WB ; Human . PubMed: 20713594
    • Palmisano GL  et al. HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol 66:1-12 (2005). PubMed: 15620456

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HLA E antibody [MEM-E/07]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Sample
    Human Cell (Human endothelial cell line)
    Specification
    Human endothelial cell line
    Permeabilization
    Yes - 0.1% triton-x 100
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 24 2013

    Western blot abreview for Anti-HLA E antibody [MEM-E/07]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (endothelial)
    Loading amount
    100 µg
    Specification
    endothelial
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 27 2012

    Flow Cytometry abreview for Anti-HLA E antibody [MEM-E/07]

     Excellent
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Human Endothelial cell line (ECV304))
    Specification
    Human Endothelial cell line (ECV304)
    Preparation
    Cell harvesting/tissue preparation method: Trypsinisation
    Sample buffer: PVG containing 0.1% trypsin
    Fixation
    2% Paraformaldehyde in PBS
    Permeabilization
    No
    Gating Strategy
    total population
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Shwetank Verma

    Verified customer

    提交于 Mar 16 2011

    Question

    I have used the HLA-E Ab MEM-E/07 (ab11820) for a work. The referees of my manuscript ask me to add a reference (published results) to clarify the specificity of the HLA-E Ab MEM-E/07 (ab11820). Can you help me.

    Read More
    Answer

    I am pleased to be able to provide you with two references: Sibilio L. et al., Tissue Antigens 62, 339, 2003. (Abstract 45 from the 3rd Int. Conference on HLA-G, Paris, July 2003). Palmisano GL et al. HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol 66:1-12 (2005). PubMed: 15620456 If you would like the pdf of the Palmisano paper I can send it to you. I hope this will help you and wish you all the best with your paper. Could you please let us know when you have published it so we can add your reference to the antibody's datasheet, and/or consider submitting an Abreview with your experience of the antibody (we reward Abreviews with points which you can use for future discounts and gifts)?

    Read More

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