Anti-HLA DR + DP + DQ 抗体 [CR3/43]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue using ab7856 at a 1/18000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human spleen.
The section was incubated with ab7856 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab7856 at a 1/18000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human skeletal muscle.
The section was incubated with ab7856 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• Flow Cyt

Lab

Flow Cytometry - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Flow cytometric analysis of KARPAS-299 (human T cell lymphoma cell) (Right) / HepG2 (human hepatocellular carcinoma epithelial cell) (Left) cells labelling HLA DR + DP + DQ with ab7856 at 1/1000 dilution (0.1&micor;g)/Red followed by a secondary antibody Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) used at a 1/5000 dilution compared with isotype control Rabbit monoclonal IgG (ab172730) / Black and unlabelled control Cell without incubation with primary antibody and secondary antibody / Blue.

Gated on viable cells.
Negative control : HepG2.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab7856 at a 1/18000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil.
The section was incubated with ab7856 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab7856 at a 1/18000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colon.
The section was incubated with ab7856 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• WB

Lab

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used as a loading control at 1/200000 dilution.

Low expression : HeLa.
Negative control : HepG2.

All lanes:

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

KARPAS-299 (human T cell lymphoma cell) whole cell lysate at 20 µg

Lane 3:

HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 30 kDa

false

Exposure time: 10s

• WB

Lab

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used as a loading control at 1/200000 dilution.

Low expression : skeletal muscle.
The identity of MW band at approximately 50 kDa (in lane 2) is unknown.

All lanes:

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 20 µg

Lane 2:

Human lung tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 30 kDa

false

Exposure time: 37s

• IHC

CiteAb

Immunohistochemistry - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Immunohistochemistry using Anti-HLA DR + DP + DQ antibody [CR3/43], ab7856. Publication image from Burdett, N. et al., 2024, Nat Commun, 39025846. Legend direct from paper.

Differences in gene expression and clinical outcomes by timing of WGD.a Hierarchical clustering of Hallmark pathway enrichment scores in the discovery ICGC cohort. n = 79. Heatmap is annotated by timing of whole genome duplication (WGD), clinical response to first line therapy and molecular subtype (C1,C2,C4,C5). b Two-sided Kaplan–Meier analysis of overall survival (top) and progression free survival (bottom) by presence and timing of WGD in 352 patients (ICGC and TCGA cohorts combined). c Top : Representative image of core stained with anti-HLA DR + DP + DQ for each WGD category; scale bar is 200 µm. Bottom : Waffle plots depicting immunohistochemistry (IHC) results, coloured by zero (yellow) versus any (lilac) staining with anti-HLA DR + DP + DQ, separated by WGD status. Early WGD n = 23 cores; Late WGD n = 49 cores; No WGD n = 37 cores. Source data are provided as a Source Data file.