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Immunology Adaptive Immunity MHC Class II

Anti-HLA DR + DP + DQ抗体[CR3/43] (ab7856)

  • Datasheet
  • SDS
Reviews (4)Q&A (7)References (15)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
  • Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)

Key features and details

  • Mouse monoclonal [CR3/43] to HLA DR + DP + DQ
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-HLA DR + DP + DQ抗体[CR3/43]
    参阅全部 HLA DR + DP + DQ 一抗
  • 描述

    小鼠单克隆抗体[CR3/43] to HLA DR + DP + DQ
  • 宿主

    Mouse
  • 特异性

    The gene of the human histocompatibility complex class II consists of at least four subregions: HLA DP, DQ, and DR, containing a minimum of one alpha and one beta chain. This antibody reacts with the beta-chain of all products of the gene subregions DP, DQ, and DR.

  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human HLA DR + DP + DQ. Raised against tonsil cells of human origin

  • 阳性对照

    • IHC-P: Human tonsil tissue
  • 常规说明


    This antibody principally labels B cells, interdigitating reticulum cells, Langerhans’ cells and manymacrophages.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein G purified
  • Primary antibody说明

    This antibody principally labels B cells, interdigitating reticulum cells, Langerhans’ cells and manymacrophages.
  • 克隆

    单克隆
  • 克隆编号

    CR3/43
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class II

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7856于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (4)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/500. Detects a band of approximately 30 kDa (predicted molecular weight: 27 kDa).
说明
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/500. Detects a band of approximately 30 kDa (predicted molecular weight: 27 kDa).

靶标

  • 功能

    Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • 序列相似性

    Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 细胞定位

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Target information above from: UniProt accession P04440 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3115 Human
    • Entrez Gene: 3117 Human
    • Entrez Gene: 3119 Human
    • Entrez Gene: 3122 Human
    • Entrez Gene: 3123 Human
    • Entrez Gene: 3125 Human
    • Entrez Gene: 3126 Human
    • Entrez Gene: 3127 Human
    • Omim: 142858 Human
    • Omim: 142860 Human
    • Omim: 142880 Human
    • Omim: 146880 Human
    • SwissProt: P01903 Human
    • SwissProt: P01913 Human
    • SwissProt: P04440 Human
    • SwissProt: P79483 Human
    • SwissProt: Q30631 Human
    • Unigene: 485130 Human
    see all
  • 别名

    • CD antibody
    • CELIAC 1 antibody
    • CELIAC1 antibody
    • DP beta 1 chain antibody
    • DP(W4) beta chain antibody
    • DPB1 antibody
    • DPB1_HUMAN antibody
    • DQ A1 antibody
    • DRB1 antibody
    • DRB4 antibody
    • FLJ27088 antibody
    • FLJ27328 antibody
    • GSE antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DR 1 beta antibody
    • HLA class II histocompatibility antigen DR alpha antibody
    • HLA class II histocompatibility antigen DR alpha chain antibody
    • HLA DP histocompatibility type beta 1 subunit antibody
    • HLA DP1B antibody
    • HLA DPB1 antibody
    • HLA DQA antibody
    • HLA DQA1 antibody
    • HLA DQB antibody
    • HLA DQB1 antibody
    • HLA DR1B antibody
    • HLA DR3B antibody
    • HLA DR4B antibody
    • HLA DRA antibody
    • HLA DRA1 antibody
    • HLA DRB1 antibody
    • HLA DRB3 antibody
    • HLA DRB4 antibody
    • HLA DRB5 antibody
    • HLA-DPB1 antibody
    • IDDM 1 antibody
    • IDDM1 antibody
    • Major histocompatibility complex class II DP beta 1 antibody
    • Major histocompatibility complex class II DQ alpha 1 antibody
    • Major histocompatibility complex class II DQ beta 1 antibody
    • Major histocompatibility complex class II DR alpha antibody
    • Major histocompatibility complex class II DR beta 1 antibody
    • Major histocompatibility complex class II DR beta 3 antibody
    • Major histocompatibility complex class II DR beta 4 antibody
    • Major histocompatibility complex class II DR beta 5 antibody
    • MGC117330 antibody
    • MHC class II antigen DPB1 antibody
    • MHC class II antigen DRA antibody
    • MHC class II HLA DQ alpha 1 antibody
    • MHC class II HLA DR beta 1 antibody
    • MHC class II HLA DR beta 3 antibody
    • MHC DPB1 antibody
    • MHC DQ beta antibody
    • MHC HLA DPB1 antibody
    • MHC HLA DQ alpha antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)

     IHC image of -HLA DR + DP + DQ staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab7856, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
    IHC image of ab7856 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7856, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
    Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
    All lanes : Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) at 1/500 dilution

    Lane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 27 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)This image is courtesy of an anonymous Abreview

    Ab7856 staining HLA DR+DP+DQ in Human Colorectal tissue sections by Immunohistochemistry. Antigen retrieval was by heat mediation at pH6 for 20minutes.  Samples were fixed with formaldehyde and blocked with 10% hydrogen peroxide for 5 minutes. Samples were incubated with primary antibody at 1/100 dilution for 15 minutes. HRP rabbit polyclonal polymer refine detection kit was used as the secondary antibody.

    See Abreview

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (15)

发表研究结果有使用 ab7856?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7856 被引用在 15 文献中.

  • Thirimanne HN  et al. Global and context-specific transcriptional consequences of oncogenic Fbw7 mutations. Elife 11:N/A (2022). PubMed: 35225231
  • Taddei RN  et al. Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages. Acta Neuropathol Commun 10:72 (2022). PubMed: 35534858
  • Gopalakrishnan S  et al. Tofacitinib Downregulates TNF and Poly(I:C)-Dependent MHC-II Expression in the Colonic Epithelium. Front Immunol 13:882277 (2022). PubMed: 35655783
  • de Vos L  et al. Characterization of B cells in lupus erythematosus skin biopsies in the context of different immune cell infiltration patterns. Front Med (Lausanne) 9:1037408 (2022). PubMed: 36438026
  • Tanno L  et al. Analysis of Immune Landscape in Pancreatic and Ileal Neuroendocrine Tumours Demonstrates an Immune Cold Tumour Microenvironment. Neuroendocrinology 112:370-383 (2022). PubMed: 34157710
View all Publications for this product

客户评价及客户问答

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1-10 of 11 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA DR + DP + DQ antibody [CR3/43]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA PH 9
Permeabilization
Yes - Tween
Specification
Tonsil
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
no additional fixation
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 11 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA DR + DP + DQ antibody [CR3/43]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
tonsil
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 12 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA DR + DP + DQ antibody [CR3/43]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Specification
Tonsil
Blocking step
Dako Protein Block X0909 as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Amanda Burnette

Verified customer

提交于 Dec 20 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA DR + DP + DQ antibody [CR3/43]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colorectal)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6 20 minutes
Permeabilization
No
Specification
Colorectal
Blocking step
hydrogen peroxide as blocking agent for 5 minute(s) · Concentration: 10%
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 16 2016

Question

What is the concentration of this antibody?

Read More

Abcam community

Verified customer

Asked on Apr 16 2012

Answer

Thank you for your inquiry.

I heard back from our lab that the concentration of all lots of ab7856 is 0.2 mg/ml. I have since added this information to the datasheet, but it may take a few days for the system to show it.

I hope this information helps. Please contact us with any other questions.

Read More

Abcam Scientific Support

回复于 Apr 16 2012

Question

 Hello, This antibody is no longer available. Which one would you recommand instead? Is there a publication showing it has already been used for immunohistochemistry on skin? Thank you in advance. Sincerely,

Read More

Abcam community

Verified customer

Asked on Dec 20 2011

Answer

Thank you for contacting us. The clone CR3/43 has already been used frequently in immunohistochemistry. I have no acces to some articles, but I think it has been used in here for skin staining: https://springerlink3.metapress.com/content/x6801633p4761hh1/resource-secured/?target=fulltext.pdf&sid=zsvhv2pbyiz1baud0nolf5c4&sh=www.springerlink.com and http://www.liebertonline.com/doi/abs/10.1089/thy.1996.6.41 Even without image, the clone has also been used in this article: http://www.springerlink.com/content/gw57180081qulj62/ We do guarantee our products to work as stated on the datasheet, and do therefore guarantee the anti HLA DR+DP+DQ antibodies, clone CR3/43, ab7856 and the ab17076 (prediluted) in immunohistochemistry on human paraffin sections (the ab17076 also on frozen sections). https://www.abcam.com/HLA-DR--DP--DQ-antibody-CR343-ab7856.html https://www.abcam.com/HLA-DR--DP--DQ-antibody-CR343-prediluted-ab17076.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

回复于 Dec 20 2011

Question

I could not find the concentration of this antibody, What is it?

Read More

Abcam community

Verified customer

Asked on Feb 04 2005

Answer

Thank you for your enquiry. We cannot provide you with a concentration for this antibody, as it is not a purified antibody. Please contact us again if you require further assistance.

Read More

Abcam Scientific Support

回复于 Feb 04 2005

Question

ANTIBODY STORAGE CONDITIONS Our customer would like to kow if there is a specific antigen retrieval protocol needed for this antibody?

Read More

Abcam community

Verified customer

Asked on Dec 12 2003

Answer

There is no specific antigen retrieval step stated on the antibody data sheet, so we recommend for Immunohistochemistry (Formalin-fixed paraffin-embedded sections)starting off by trying the heat mediated pressure cooker method.

Read More

Abcam Scientific Support

回复于 Dec 12 2003

Question

I wanted to ask you whether you have the unconjugated CR3/43 antibody without Na-azide and what would be in this case the price of this antibody.

Read More

Abcam community

Verified customer

Asked on Dec 08 2003

Answer

Thank you for your enquiry. Ab7856 is only available as described on the online datasheet. To see if another company may offer a CR3/43 antibody without Na azide, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249).

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Abcam Scientific Support

回复于 Dec 08 2003

Question

Do you know if this ab is cross-reactive with non-human primates, specifically rhesus monkeys?

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Abcam community

Verified customer

Asked on Oct 13 2003

Answer

We are very sorry but there is no information about the cross-reactivity with rhesus monkeys. All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). We will update the on-line datasheet of this product as soon as get more data.

Read More

Asdf Edo

Abcam Scientific Support

回复于 Oct 13 2003

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