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重组RabMAb

重组Anti-HLA Class 1 ABC抗体[EPR22172] (ab225636)

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Immunohistochemistry (Frozen sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunohistochemistry (Frozen sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Western blot - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunoprecipitation - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22172] to HLA Class 1 ABC
  • Suitable for: IHC-Fr, WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free

概述

  • 产品名称

    Anti-HLA Class 1 ABC抗体[EPR22172]
    参阅全部 HLA Class 1 ABC 一抗

  • 描述

    兔单克隆抗体[EPR22172] to HLA Class 1 ABC

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-Fr, WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: THP-1 and Raji cells. Flow Cyt: Raji and THP-1 cells. IHC-P: Human colonic carcinoma, stomach and liver tissue. WB: Raji, THP-1, HL-60, Jurkat, HepG2, TF-1 and Ramos whole cell lysate. Human tonsil. Recombinant human HLA-A protein (aa24-aa365). Recombinant human HLA-C protein (aa25-aa308). IP: HLAB in THP-1 whole cell lysate. IHC-Fr: Human frozen spleen and liver tissue sections.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR22172

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab225636于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use a concentration of 0.05 µg/ml.
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
Flow Cyt
1/600.
IP
1/30.
说明
IHC-Fr
Use a concentration of 0.05 µg/ml.
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
Flow Cyt
1/600.
IP
1/30.

靶标

  • 相关性

    HLA-ABC antigen is present on most nucleated cells of humans and Old World monkeys. HLA CLass I is involved in the presentation of foreign antigens to the immune system.

  • 细胞定位

    Cell Membrane; Single-pass type I membrane protein

  • 数据库链接

    • 别名

      • Antigen Presenting Molecule antibody
      • Cw*1701 antibody
      • D6S204 antibody
      • FLJ27082 antibody
      • HLA A antibody
      • HLA B antibody
      • HLA C antibody
      • HLA class 1 A antibody
      • HLA class 1 B antibody
      • HLA class 1 C antibody
      • HLA JY3 antibody
      • HLAA antibody
      • HLAB antibody
      • HLAC antibody
      • HLC C antibody
      • Human Leucocyte Antigen C antibody
      • Major Histocompatibility Complex Class I A antibody
      • Major Histocompatibility Complex Class I B antibody
      • Major Histocompatibility Complex Class I C antibody
      • Major histocompatibility complex, class I, A + B + C antibody
      • MHC class I antigen A*1 antibody
      • MHC Class I Antigen antibody
      • MHC class I antigen B*7 antibody
      • MHC class I antigen Cw*1 antibody
      • MHC class I HLA A antibody
      • MHC class I HLA B antibody
      • MHC class I HLA C antibody
      • MHC HLA ABC antibody
      • PSORS1 antibody
      see all

    图片

    • Immunohistochemistry (Frozen sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunohistochemistry (Frozen sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)Lab

      IHC image of HLA Class 1 ABC staining in a section of human normal frozen liver* performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab225636, 0.05ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Frozen sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunohistochemistry (Frozen sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)Lab

      IHC image of HLA Class 1 ABC staining in a section of human normal frozen spleen* performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab225636, 0.05ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunocytochemistry/ Immunofluorescence - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Immunofluorescent analysis of 100% methanol-fixed THP-1 (human monocytic leukemia cell line) cells labeling HLAB with ab225636 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in THP-1 cells. The nuclear counterstain is DAPI (blue).
      Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).
      The negative control is the secondary antibody only.

      Methanol is recommended for fixation as weak signal was observed using 4% PFA.

    • Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cells labeling HLAB with ab225636 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling HLAB with ab225636 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining on lymph nodule of human stomach (PMID:25294906) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Western blot - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Western blot - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      All lanes : Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636) at 1/1000 dilution

      Lane 1 : Raji (human Burkitt's lymphoma cell line), whole cell lysate
      Lane 2 : THP-1 (human monocytic leukemia cell line), whole cell lysate
      Lane 3 : HL-60 (human promyelocytic leukemia cell line), whole cell lysate
      Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood), whole cell lysate
      Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line), whole cell lysate
      Lane 6 : TF-1 (human bone marrow erythroleukemia cell line), whole cell lysate
      Lane 7 : Ramos (human Burkitt's lymphoma cell line), whole cell lysate
      Lane 8 : Human tonsil

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 40 kDa
      Observed band size: 40 kDa


      Exposure time: 26 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Weak reactive bands of undetermined proteins are observed in addition to the target band.

       

    • Immunoprecipitation - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunoprecipitation - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      HLAB was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia cell line) whole cell lysate with ab225636 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab225636 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
      Lane 1: THP-1 whole cell lysate 10 µg (Input).
      Lane 2: ab225636 IP in THP-1 whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225636 in THP-1 whole cell lysate.
      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 3 seconds.

    • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunocytochemistry/ Immunofluorescence - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) cells labeling HLAB with ab225636 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Raji cells. The nuclear counterstain is DAPI (blue).
      Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).
      The negative control is the secondary antibody only.

      Methanol is recommended for fixation as weak signal was observed using 4% PFA.

    • Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling HLAB with ab225636 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Immunohistochemical analysis of paraffin-embedded human liver tissue labeling HLAB with ab225636 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on hepatic sinus endothelium of human liver (PMID: 26963506) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue labeling HLAB with ab225636 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining on tumor cells of human colonic carcinoma (PMID: 26310568, PMID: 17014712) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (4)

    发表研究结果有使用 ab225636？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab225636 被引用在 4 文献中.

    • Zhou J  et al. The immune checkpoint molecule, VTCN1/B7-H4, guides differentiation and suppresses proinflammatory responses and MHC class I expression in an embryonic stem cell-derived model of human trophoblast. Front Endocrinol (Lausanne) 14:1069395 (2023). PubMed: 37008954
    • Lian W  et al. FABP6 Expression Correlates with Immune Infiltration and Immunogenicity in Colorectal Cancer Cells. J Immunol Res 2022:3129765 (2022). PubMed: 36033394
    • Zhou Q  et al. Identification and validation of a poor clinical outcome subtype of primary prostate cancer with Midkine abundance. Cancer Sci 113:3698-3709 (2022). PubMed: 36018546
    • Shen R  et al. Influence of oncogenic mutations and tumor microenvironment alterations on extranodal invasion in diffuse large B-cell lymphoma. Clin Transl Med 10:e221 (2020). PubMed: 33252851

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