Anti-HLA Class 1 ABC抗体[EMR8-5] (ab70328)
![Western blot - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)](https://www.abcam.cn/ps/products/70/ab70328/Images/ab70328-84890-anti-hla-class-1-abc-antibody-emr8-5-western-blot.jpg "Western blot - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)")
Key features and details
- Mouse monoclonal [EMR8-5] to HLA Class 1 ABC
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-HLA Class 1 ABC抗体[EMR8-5]
参阅全部 HLA Class 1 ABC 一抗 -
描述
小鼠单克隆抗体[EMR8-5] to HLA Class 1 ABC -
宿主
Mouse -
特异性
ab70328 reacts with the heavy chains of human HLA class 1 A, -B, and -C. -
经测试应用
适用于: Flow Cyt, IHC-P, WBmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to HLA Class 1 ABC. Recombinant HLA-A*2402 extracellular domain
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阳性对照
- IHC-P: Human tonsil tissue sections
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EMR8-5 -
骨髓瘤
NS1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70328于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | (1) |
Use 1-2µg for 106 cells.
(methanol or paraformaldehyde fixed cells) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| IHC-P | (9) |
Use a concentration of 0.05 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB | (2) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Flow Cyt
Use 1-2µg for 106 cells. (methanol or paraformaldehyde fixed cells) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P
Use a concentration of 0.05 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use at an assay dependent concentration. |
靶标
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相关性
HLA-ABC antigen is present on most nucleated cells of humans and Old World monkeys. HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
细胞定位
Cell Membrane; Single-pass type I membrane protein -
数据库链接
- Entrez Gene: 3105 Human
- Entrez Gene: 3106 Human
- Entrez Gene: 3107 Human
- SwissProt: P01889 Human
- SwissProt: P30443 Human
- SwissProt: P30499 Human
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别名
- Antigen Presenting Molecule antibody
- Cw*1701 antibody
- D6S204 antibody
see all
图片
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Western blot - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)All lanes : Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)
Lane 1 : A*2402
Lane 2 : A*0101
Lane 3 : A*1101
Lane 4 : A*0201
Lane 5 : A*0207
Lane 6 : A2402
Lane 7 : B*0702
Lane 8 : B*0801
Lane 9 : B*1501
Lane 10 : B*3501
Lane 11 : B*4001
Lane 12 : B*4002
Lane 13 : B*4006
Lane 14 : B*4403
Lane 15 : Cw*0102
Lane 16 : Cw*0801
Lane 17 : Cw*1202
Lane 18 : Cw*1502Western blot analysis of ab70328 reactivity using recominant HLA heavy chains.
Lanes 1-6: HLA-A
Lanes 7-14: HLA-B
Lanes 15-18: HLA-C
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![Western blot - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)](https://www.abcam.cn/ps/products/70/ab70328/Images/ab70328-84892-anti-hla-class-1-abc-antibody-emr8-5-western-blot.jpg)
Western blot - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)All lanes : Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)
Lane 1 : Human oral cancer cell line OSC20
Lane 2 : HLA-A2402 gene transfected OSC20
Lane 3 : Human Leukemia cell line K562
Lane 4 : Recombinant HLA-A2402 heavy chain -
![Flow Cytometry - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)](https://www.abcam.cn/ps/products/70/ab70328/Images/ab70328-84889-anti-hla-class-1-abc-antibody-emr8-5-flow-cytometry.jpg)
Flow Cytometry - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)Overlay histogram showing Jurkat cells stained with ab70328 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70328, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)](https://www.abcam.cn/ps/products/70/ab70328/Images/ab70328-448955-antihlaclass1abcantibodyimmunohistochemistrytonsilhuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)IHC image of HLA Class 1 ABC staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab70328, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (152)
ab70328 被引用在 152 文献中.
- Godfrey J et al. Pembrolizumab for the treatment of disease relapse after allogeneic hematopoietic stem cell transplantation. Blood Adv 7:963-970 (2023). PubMed: 35973200
- Bhatia V et al. Targeting advanced prostate cancer with STEAP1 chimeric antigen receptor T cell and tumor-localized IL-12 immunotherapy. Nat Commun 14:2041 (2023). PubMed: 37041154
- Noviello TMR et al. Guadecitabine plus ipilimumab in unresectable melanoma: five-year follow-up and integrated multi-omic analysis in the phase 1b NIBIT-M4 trial. Nat Commun 14:5914 (2023). PubMed: 37739939
- Yarmarkovich M et al. Targeting of intracellular oncoproteins with peptide-centric CARs. Nature 623:820-827 (2023). PubMed: 37938771
- Kruse B et al. CD4+ T cell-induced inflammatory cell death controls immune-evasive tumours. Nature 618:1033-1040 (2023). PubMed: 37316667