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Anti-HLA A2抗体[BB7.2] - BSA and Azide free (ab74674)

Submit a review Q&A (3)References (8)
Flow Cytometry - Anti-HLA A2 antibody [BB7.2] - BSA and Azide free (ab74674)
  • Flow Cytometry - Anti-HLA A2 antibody [BB7.2] - BSA and Azide free (ab74674)

Key features and details

  • Mouse monoclonal [BB7.2] to HLA A2 - BSA and Azide free
  • Suitable for: IP, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 FITC PE

概述

  • 产品名称

    Anti-HLA A2抗体[BB7.2] - BSA and Azide free
    参阅全部 HLA A2 一抗

  • 描述

    小鼠单克隆抗体[BB7.2] to HLA A2 - BSA and Azide free

  • 宿主

    Mouse

  • 特异性

    This product has been purified from tissue culture supernatant.

  • 经测试应用

    适用于: IP, Flow Cytmore details
    不适用于: WB

  • 种属反应性

    与反应: Human

  • 免疫原

    Full length protein corresponding to Human HLA A2. HLA-A2 solubilised by papain Full length Native protein

  • 表位

    The C-terminus of alpha-2 helix and a turn on one of the underlying beta strands.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.40
    Constituent: PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    This product has been purified from tissue culture supernatant. Purity >95% by SDS-PAGE.

  • 克隆

    单克隆

  • 克隆编号

    BB7.2

  • 同种型

    IgG2b

  • 轻链类型

    kappa

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab74674于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent concentration.
Flow Cyt
Use 2µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
说明
IP
Use at an assay dependent concentration.
Flow Cyt
Use 2µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
应用说明
Is unsuitable for WB.

靶标

  • 相关性

    Involved in the presentation of foreign antigens to the immune system. Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein.

  • 细胞定位

    Membrane; Single-pass type I membrane protein.

  • 数据库链接

    • 别名

      • Antigen presenting molecule antibody
      • HLA A antibody
      • HLA A histocompatibility type antibody
      • HLA antibody
      • HLA class I antibody
      • HLA class I histocompatibility antigen A 2 alpha chain antibody
      • HLA class I histocompatibility antigen A2 alpha chain antibody
      • HLA-A antibody
      • HLAA antibody
      • Human leukocyte antigen antibody
      • Leucocyte antigen class I antibody
      • Leukocyte antigen class I A antibody
      • Major histocompatibility complex class I A antibody
      • Major histocompatibility complex class I antibody
      • MHC class I antigen [precursor] antibody
      • MHC class I antigen A antibody
      • MHC class I antigen A*2 antibody
      • MHC class I antigen A2 antibody
      • MHC class I antigen HLA A antibody
      • MHC class I antigen HLA A heavy chain antibody
      • MHC leukocyte antigen antibody
      see all

    图片

    • Flow Cytometry - Anti-HLA A2 antibody [BB7.2] - BSA and Azide free (ab74674)
      Flow Cytometry - Anti-HLA A2 antibody [BB7.2] - BSA and Azide free (ab74674)
      Flow cytometry of human peripheral blood cells with ab74674 at 2 µg/ml
    • Flow Cytometry - Anti-HLA A2 antibody [BB7.2] - BSA and Azide free (ab74674)
      Flow Cytometry - Anti-HLA A2 antibody [BB7.2] - BSA and Azide free (ab74674)
      Overlay histogram showing Raji cells stained with ab74674 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74674, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Raji cells used under the same conditions.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    文献 (8)

    发表研究结果有使用 ab74674？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab74674 被引用在 8 文献中.

    • Chen Z  et al. T and B cell Epitope analysis of SARS-CoV-2 S protein based on immunoinformatics and experimental research. J Cell Mol Med 25:1274-1289 (2021). PubMed: 33325143
    • Choi J  et al. Systematic discovery and validation of T cell targets directed against oncogenic KRAS mutations. Cell Rep Methods 1:100084 (2021). PubMed: 35474673
    • Pearson CS  et al. Identification of a critical sulfation in chondroitin that inhibits axonal regeneration. Elife 7:N/A (2018). PubMed: 29762123
    • Li D  et al. Development of a T-cell Receptor Mimic Antibody against Wild-Type p53 for Cancer Immunotherapy. Cancer Res 77:2699-2711 (2017). PubMed: 28363997
    • Shafaie S  et al. Influence of surface geometry on the culture of human cell lines: A comparative study using flat, round-bottom and v-shaped 96 well plates. PLoS One 12:e0186799 (2017). PubMed: 29084285
    • Soysal SD  et al. Identification and translational validation of novel mammaglobin-A CD8 T cell epitopes. Breast Cancer Res Treat 147:527-37 (2014). PubMed: 25212176
    • Tomita Y  et al. Peptides derived from human insulin-like growth factor-II mRNA binding protein 3 can induce human leukocyte antigen-A2-restricted cytotoxic T lymphocytes reactive to cancer cells. Cancer Sci 102:71-8 (2011). PubMed: 21087352
    • Qu C  et al. MHC class I/peptide transfer between dendritic cells overcomes poor cross-presentation by monocyte-derived APCs that engulf dying cells. J Immunol 182:3650-9 (2009). PubMed: 19265143

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Question

    code pour l'abtrial pour ab74674 en WB

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    Answer

    Code de remise :xxxxxxx
    Date d’expiration : 08/04/12

    Je suis ravie que vous souhaitiez participer a notre offre et tester ab74674 en western blot. Le code de réduction correspondant est xxxxxxxxx. Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre produit.

    Vous devez dans un premier temps soumettre une Abreview avec vos résultatsen western blot en notant ce code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de ab74674.

    Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches.

    Termes et Conditions : www.abcam.com/collaborationdiscount.

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    Question

    abtrial pour ab74674 en western blot

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    Answer

    Merci de votre intérêt pour ab74674. A ma connaissance, cet anticorps n’a pas encore été testé en western blot et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester ab74674en western blot, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de ab74674 pourra être utilisable lors d’une prochaine commande.

    Etapes à suivre :

    1. Nous confirmer que vous souhaiteriez tester ab74674en western blot afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab74674

    2. Acheter ab74674 par téléphone, fax ou internet (www.abcam.com)

    3. Le tester en western blot

    4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews

    5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un produit.
    Même si ab74674 est démontré ne pas fonctionneren western blot, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochaine commande.

    N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

    Termes et Conditions : https://www.abcam.com/collaborationdiscount.

    En ce concerne les remises, ma collègue vous a envoyé un mail expliquant que pour l’achat de 5/10/20 unités du produit, vous aurez une remise de xxxxxxxx respectivement.

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Monoclonal de lapin gratuit pour tout achat d'anticorps primaire, dans la limite des stocks disponibles. Notez le code "RABMAB-XBSMG" sur votre prochaine commande d’anticorps primaire. Pour plus d'informations, visitez le lien suivant: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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    Question

    The Ab reported into this paper is not ab74674 but the FITC conjugated ab27728.
    Kind regards

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    Answer

    Thank you for contacting us.

    Unfortunately we have not determined which specific HLA-A2 allele(s) this clone binds. However, it is clear from the paper you kindly provided that the clone binds HLA-A201. Unfortunately, we currently do not have any empirical data to indicate if this clone is specific just to the HLA-A201 allele or if detects other alleles.

    http://www.ncbi.nlm.nih.gov/pubmed/19265143

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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