重组Anti-HJURP抗体[EPR22619-41]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody [EPR22619-41] (AB233541)

Immunohistochemical analysis of paraffin-embeddded human thymus tissue labeling HJURP with ab233541 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on thymocytes of human thymus cortex (PMID : 17256767) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

The section was incubated with ab233541 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody [EPR22619-41] (AB233541)

Immunohistochemical analysis of paraffin-embeddded human tonsil tissue labeling HJURP with ab233541 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human tonsil germinal center is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

The section was incubated with ab233541 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• WB

Unknown

Western blot - Anti-HJURP antibody [EPR22619-41] (AB233541)

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 23771058).

All lanes:

Western blot - Anti-HJURP antibody [EPR22619-41] (ab233541) at 1/1000 dilution

All lanes:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

false

Exposure time: 3min

• WB

Unknown

Western blot - Anti-HJURP antibody [EPR22619-41] (AB233541)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lanes 1-3 : 48 seconds; Lane 4 : 3.25 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 23771058).

Lanes 1-3 in this blot were developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-HJURP antibody [EPR22619-41] (ab233541) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HeLa nuclear fraction at 20 µg

Lane 3:

HeLa cytoplasmic fraction at 20 µg

Lane 4:

Human tonsil tissue lysate at 20 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 4:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

false