AB100800

Anti-HJURP抗体

Name: HJURP抗体 (ab100800)| Abcam中文官网
Brand: Abcam Limited
SKU: AB100800
Price: 4826 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-HJURP antibody

(1 Review)

(10 Publications)

Rabbit Polyclonal HJURP antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human HJURP aa 650-700.

查看别名

FAKTS, FLEG1, URLC9, HJURP, Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody (AB100800)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody (AB100800)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling HJURP with ab100800 at 1/5000 (0.2μg/mg). Detection : DAB.

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Immunoprecipitation - Anti-HJURP antibody (AB100800)

ab100800 at 1 µg/ml staining HJURP in HeLa cell lysate immunoprecipitated using ab100800 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time : 30 seconds

All lanes:

Immunoprecipitation - Anti-HJURP antibody (ab100800)

Predicted band size: 84 kDa

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Unknown

Western blot - Anti-HJURP antibody (AB100800)

All lanes:

Western blot - Anti-HJURP antibody (ab100800) at 0.04 µg/mL

Lane 1:

K-562 whole cell lysate at 2 µg

Lane 2:

U2OS whole cell lysate at 2 µg

Lane 3:

HeLa whole cell lysate at 2 µg

Lane 4:

MCF-7 whole cell lysate at 2 µg

Predicted band size: 84 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P, IP

applications

免疫原

Synthetic Peptide within Human HJURP aa 650-700. The exact immunogen used to generate this antibody is proprietary information.

Q8NCD3

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04 µg/mL", "WB-species-notes": "" } } }

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产品详情

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified using an epitope specific to HJURP immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HJURP also known as Holliday junction recognition protein plays an important mechanical role in chromatin assembly. This protein weighs approximately 82 kDa and coordinates the correct loading of CENP-A at the centromeres during S phase and early G1 phase of the cell cycle. HJURP is mostly expressed in proliferative tissues and cell lines reflecting its involvement in processes requiring accurate chromosomal segregation during cell division. Its presence ensures that centromere identity is passed onto the daughter cells.

Biological function summary

HJURP is critical for centromere function and stability. It operates by forming a complex with centromere protein A (CENP-A) which completes the assembly and maintenance of centromere chromatin. HJURP achieves this by acting as a chaperone specifically ensuring the accurate deposition of CENP-A at the centromeres. The functionality of HJURP within this complex highlights its role in sustaining genomic integrity throughout cell division processes.

Pathways

HJURP lies at the center of the centromere-specific chromatin assembly pathway. This pathway is essential for the replenishment of centromeric CENP-A thereby ensuring accurate chromosome segregation. HJURP interacts significantly with other proteins such as CALM and H4 aiding in chromatin dynamics and stability. These interactions help integrate HJURP's actions into larger cellular and genetic processes particularly those involving DNA replication and cell cycle progression.

HJURP links closely to cancerous transformations and chromosomal instability disorders. Abnormal expression or mutations of HJURP can disrupt centromere function potentially leading to aneuploidy—a condition often found in various cancers. Its association with other proteins such as HJURP's interaction with overexpressed CENP-A in these pathological states highlights its significance in maintaining cellular health. Therefore HJURP emerges as a potential biomarker for these conditions and warrants further research into therapeutic interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.

See full target information HJURP

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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 77:103392 PubMed39405980

2024

HJURP inhibits sensitivity to ferroptosis inducers in prostate cancer cells by enhancing the peroxidase activity of PRDX1.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Lai,Weian Zhu,Jianjie Wu,Jiongduan Huang,Xiaojuan Li,Yun Luo,Yu Wang,Hengda Zeng,Mingqiang Li,Xiaofu Qiu,Xingqiao Wen

Biomedicines 12: PubMed39200236

2024

The Impact of DAXX, HJURP and CENPA Expression in Uveal Melanoma Carcinogenesis and Associations with Clinicopathological Parameters.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandros Pergaris,Georgia Levidou,Georgios Mandrakis,Maria-Ioanna Christodoulou,Michail V Karamouzis,Jerzy Klijanienko,Stamatios Theocharis

Frontiers in oncology 12:972550 PubMed35965590

2022

The expression, clinical relevance, and prognostic significance of HJURP in cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Jinyan Yuan,Zhenzhong Liu,Wenwen Cao,Pei Liu

International journal of molecular sciences 23: PubMed35955489

2022

Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Georgia Levidou,Konstantinos Palamaris,Alexandros G Sykaras,Georgios Andreadakis,Christos Masaoutis,Irene Theochari,Penelope Korkolopoulou,Dimitra Rontogianni,Stamatios Theocharis

Cell death & disease 12:583 PubMed34099634

2021

HJURP promotes proliferation in prostate cancer cells through increasing CDKN1A degradation via the GSK3β/JNK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Lai,Weian Zhu,Chutian Xiao,Xiaojuan Li,Yu Wang,Yuefu Han,Jiayu Zheng,Yingqiu Li,Mingqiang Li,Xingqiao Wen

Oncology reports 44:1246-1254 PubMed32582972

2020

Holliday junction‑recognition protein modulates apoptosis, cell cycle arrest and reactive oxygen species stress in human renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-Shui Yuan,Zeng-Sheng Chen,Ke Wang,Zong-Liang Zhang

Nature communications 10:4046 PubMed31492860

2019

Mechanism of centromere recruitment of the CENP-A chaperone HJURP and its implications for centromere licensing.

Applications

Unspecified application

Species

Unspecified reactive species

Dongqing Pan,Kai Walstein,Annika Take,David Bier,Nadine Kaiser,Andrea Musacchio

The Journal of cell biology 213:415-24 PubMed27185834

2016

Diaphanous formin mDia2 regulates CENP-A levels at centromeres.

Applications

Unspecified application

Species

Unspecified reactive species

Chenshu Liu,Yinghui Mao

Molecular oncology 9:657-74 PubMed25497280

2014

The histone chaperone HJURP is a new independent prognostic marker for luminal A breast carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rocío Montes de Oca,Zachary A Gurard-Levin,Frédérique Berger,Haniya Rehman,Elise Martel,Armelle Corpet,Leanne de Koning,Isabelle Vassias,Laurence O W Wilson,Didier Meseure,Fabien Reyal,Alexia Savignoni,Bernard Asselain,Xavier Sastre-Garau,Geneviève Almouzni

BioMed research international 2014:159765 PubMed25243117

2014

New biomarkers to predict the evolution of in situ breast cancers.

Applications

IHC

Species

Human

S Bravaccini,M M Tumedei,E Scarpi,W Zoli,C Rengucci,L Serra,A Curcio,F Buggi,S Folli,A Rocca,R Maltoni,M Puccetti,D Amadori,R Silvestrini

View all publications

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Anti-HJURP抗体

Anti-HJURP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody (AB100800)

Immunoprecipitation - Anti-HJURP antibody (AB100800)

Western blot - Anti-HJURP antibody (AB100800)