Anti-HIVEP2抗体[2418C2a] (ab70599)
Key features and details
- Mouse monoclonal [2418C2a] to HIVEP2
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
-
产品名称
Anti-HIVEP2抗体[2418C2a] -
描述
小鼠单克隆抗体[2418C2a] to HIVEP2 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant fragment of human HIVEP2 from near the C terminus.
-
阳性对照
- Recombinant human HIVEP2.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate -
Concentration information loading...
-
纯度
Protein G purified -
纯化说明
ab70599 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
克隆
单克隆 -
克隆编号
2418C2a -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70599于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
说明 |
---|
WB: Use at an assay dependent dilution. Detects bands of approximately 35 and 39 kDa (predicted molecular weight: 269 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
-
功能
This protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, somatostatin receptor II, and interferon-beta genes. It may act in T-cell activation. -
组织特异性
Expressed in brain and skeletal muscle. -
序列相似性
Contains 4 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
-
别名
- HIV EP2 antibody
- HIV-EP2 antibody
- HIVEP2 antibody
see all
图片
-
Anti-HIVEP2 antibody [2418C2a] (ab70599) + immunising recombinant protein
Predicted band size: 269 kDa
Observed band size: 35,39 kDa why is the actual band size different from the predicted?
The molecular weight of the bands on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
数据表及文件
-
Datasheet download
文献 (1)
ab70599 被引用在 1 文献中.
- Bash-Imam Z et al. Translational reprogramming of colorectal cancer cells induced by 5-fluorouracil through a miRNA-dependent mechanism. Oncotarget 8:46219-46233 (2017). PubMed: 28515355