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Anti-HIVEP1抗体[2417C2 a] (ab58652)

Submit a review Q&A (4)
Western blot - Anti-HIVEP1 antibody [2417C2 a] (ab58652)

    Key features and details

    • Mouse monoclonal [2417C2 a] to HIVEP1
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-HIVEP1抗体[2417C2 a]

    • 描述

      小鼠单克隆抗体[2417C2 a] to HIVEP1

    • 宿主

      Mouse

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Recombinant fragment
      预测可用于: Human

    • 免疫原

      Recombinant C terminal fragment of human HIVEP1

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab58652于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 297 kDa.
    说明
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 297 kDa.

    靶标

    • 功能

      This protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV-1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, and interferon-beta genes. It may act in T-cell activation. Involved in activating HIV-1 gene expression. Isoform 2 and isoform 3 also bind to the IPCS (IRF1 and p53 common sequence) DNA sequence in the promoter region of interferon regulatory factor 1 and p53 genes and are involved in transcription regulation of these genes. Isoform 2 does not activate HIV-1 gene expression. Isoform 2 and isoform 3 may be involved in apoptosis.

    • 序列相似性

      Contains 5 C2H2-type zinc fingers.

    • 结构域

      Contains two sets of 2 zinc-fingers, which are widely separated and recognize the same DNA sequence. There is a fifth zinc-finger in-between.

    • 细胞定位

      Cytoplasm. Nucleus and Nucleus.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Cirhin interaction protein antibody
        • CIRIP antibody
        • CRYBP1 antibody
        • GAAP antibody
        • Gate keeper of apoptosis-activating protein antibody
        • HIV EP1 antibody
        • HIV-EP1 antibody
        • HIVEP 1 antibody
        • Hivep1 antibody
        • Human immunodeficiency virus type I enhancer binding protein 1 antibody
        • Human immunodeficiency virus type I enhancer-binding protein 1 antibody
        • Major histocompatibility complex binding protein 1 antibody
        • Major histocompatibility complex-binding protein 1 antibody
        • MBP 1 antibody
        • MBP-1 antibody
        • Positive regulatory domain II binding factor 1 antibody
        • Positive regulatory domain II-binding factor 1 antibody
        • PRDII BF1 antibody
        • PRDII-BF1 antibody
        • Schnurri 1 antibody
        • ZAS1 antibody
        • ZEP1_HUMAN antibody
        • Zinc finger protein 40 antibody
        • ZNF40 antibody
        • ZNF40A antibody
        see all

      图片

      • Western blot - Anti-HIVEP1 antibody [2417C2 a] (ab58652)
        Western blot - Anti-HIVEP1 antibody [2417C2 a] (ab58652)
        Anti-HIVEP1 antibody [2417C2 a] (ab58652) + immunising recombinant protein fragment

        Predicted band size: 297 kDa
        Observed band size: 34 kDa why is the actual band size different from the predicted?



        The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

      文献 (0)

      发表研究结果有使用 ab58652？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab58652 尚未被引用在任何文献中。

      客户评价及客户问答

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      1-4 of 4 Abreviews or Q&A

      Question

      Estimada ,
      en referencia al mail anterior, le envio una imagen de mi western realizado con Anti-HIVEP1 antibody [2417C2 a] (ab58652) en lisado celular de Huvec. En dicha imagen puede observar la banda de 34 KDa correspondiente a Hivep1 y otra banda mucho mas tenue de aproximadamente 22 KDa. Como le dije en el mail anterior, esperaria obtener una banda de 297 KDa correspondiente a la proteina endogena. Tiene alguna idea de porque obtengo justamente la de 34 KDa, cuando este tamano corresponde al fragmento recombinante que habeis usado para validar vuestro anticuerpo? Dudo que sea el resultado de degradacion proteica, pues en tal caso esperaria encontrar mas bandas de diferentes tamanos.
      Gracias por su ayuda!

      Read More
      Answer

      Gracias por tu respuesta, y por enviar las imágenes.

      Dado que en nuestro laboratorio no se ha testado el anticuerpo con la proteína endógena, me temo que no tengo evidencias para asegurar que la banda a 34KDa corresponde a un fragmento recombinante o a un producto de degradación proteica.

      Quizás aislando fracciones celulares para realizar la detección, como la fracción citoplásmica, o la nuclear, y usar un buffer de lisis como RIPA ayuden a dilucidar si realmente es un producto de degradación.

      Al no tener mucha información disponible sobre la caracterización de este anticuerpo, la garantía Abpromise lo cubre si no da el resultado esperado en vuestras células.

      Así pues, siendo que no hay alternativas contra HIVEP1, puedo o bien rembolsaros el importe del anticuerpo, o enviaros un reemplazo gratuito de otro anticuerpo del catálogo. También puedo emitir una nota de abono para futuros pedidos.

      Además, podéis aprovechar la ocasión para beneficiaros de la promoción de RabMabs actualmente vigente, por la que al realizar el envío de cualquier anticuerpo primario, de forma completamente gratuita se os envía además un RabMab. Para más información picha https://www.abcam.com/index.html?pageconfig=resource&rid=15447.

      Espero entonces tu respuesta para que me indiques cómo proceder.

      Que pases un buen día.

      Read More

      Question

      Dear scientific support team, I am using your Anti-HIVEP1 antibody [2417C2 a] (ab58652) in HUVEC cells. I obtain one band at 34KDa at 1/400 antibody dilution and under reducing conditions (1% b-mercaptoetanol in the LB4X). This band corresponds to the observed band you show in the image of this product code. However, you indicate that this observed band is the result of a recombinat fragment of the protein Hivep1. Also, you indicate that the expected size is 297KDa, which corresponds to the molecular weight of the natural Hivep1. Accordingly, I understand that I should detect a band of 297KDa, which corresponds to my entire protein and not to the recombinant fragment. However, I always see the band at 34KDa and I do not understand why? Please, could you solve me this doubt? Thank you!

      Read More
      Answer

      Gracias por contactarnos.

      Espero que no te importe que te conteste en castellano, ya que recientemente hemos inaugurado el Servicio Técnico en español. Si prefirieses seguir contactándonos en inglés, no dudes en comunicármelo.

      Siento que los resultados observados no se corresponden con lo esperado.

      He confirmado con mis compañeros en el laboratorio que este anticuerpo solo se ha testado con un fragmento de la proteína recombinante, al no haberlo testado con la proteína endógena no podemos ofrecer la garantía de que el anticuerpo detecte la secuencia entera de la proteína.

      Si te parece, para salir de dudas, y si estáis interesados, puedo comprobar si el péptido utilizado está disponible para que lo utilicéis para bloquear en vuestro western.

      Me temo que no tenemos ningún otro anticuerpo en el catálogo para detectar HIVEP1.

      Siento mucho no poder ser de más ayuda. No dudes en contactarme para cualquier otra consulta.

      Te informo que actualmente tenemos una promoción por la que al comprar cualquier anticuerpo primario os regalamos otro primario Rabbit Monoclonal. Simplemente indica el código RABMAB-XBSMG en el próximo pedido. Para más información picha https://www.abcam.com/index.html?pageconfig=resource&rid=15447.

      Read More

      Question

      Dear technical support team,   thanks a lot for your answer regarding the HIVEP1 antibody ab58652! I have another question: Do you have information about using this antibody for paraffin probes?   Thank you very much for your effort!  

      Read More
      Answer

      Thank you very much for your interest in ab58652. To our knowledge, ab58652 has not been tested in immunohistochemistry on paraffin-embedded sections (IHC-P) yet. However, we do not see a reason why it should not work. Therefore, I can offer a discount off a future purchase if you buy ab58652 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab58652 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab58652 so please wait for my reply before ordering. 2. Purchase ab58652 either by phone, fax, or online (www.abcam.com). 3. Test it in IHC-P. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab58652 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further (gerne auch auf Deutsch). The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Question

      I am using an antibody against HIVEP1 (mouse monoclonal 2417C2 a, ab58652). Since there exist different HIVEP1 isoforms, I would appreciate if you let me know about the region/epitop/amino acid sequence the HIVEP1 antibody recognizes.

      Read More
      Answer

      Thank you for contacting us. The immunogen is a C-terminal fragment within residues 2550 and the C-terminus of isoform 1 - based on the UniProt database entry for the human protein P15822. http://www.uniprot.org/uniprot/P15822 The exact epitope which is usually between 5-10 residues long, has not been mapped. Based on the immunogen sequence, also isoforms 2 and 3 will be recognized by the antibody. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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