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Anti-HIV1 tat抗体[1] (ab42359)

Submit a review Q&A (4)References (11)
Western blot - Anti-HIV1 tat antibody [1] (ab42359)

    Key features and details

    • Mouse monoclonal [1] to HIV1 tat
    • Suitable for: Radioimmunoprecipitation, IHC-P, ELISA, WB
    • Reacts with: Human immunodeficiency virus
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-HIV1 tat抗体[1]
      参阅全部 HIV1 tat 一抗

    • 描述

      小鼠单克隆抗体[1] to HIV1 tat

    • 宿主

      Mouse

    • 特异性

      This antibody reacts with the rTat consensus sequences of HIV1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE).

    • 经测试应用

      适用于: Radioimmunoprecipitation, IHC-P, ELISA, WBmore details

    • 种属反应性

      与反应: Human immunodeficiency virus

    • 免疫原

      Recombinant fragment corresponding to HIV-1 HIV1 tat.

    • 表位

      Mapped to amino acids 2-9 (EPVDPRLE - B subtype consensus).

    • 常规说明

      Also known as clone 02-001.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab42359于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Radioimmunoprecipitation
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    ELISA
    Use a concentration of 0.02 - 0.05 µg/ml.
    WB
    Use a concentration of 1 - 2 µg/ml.
    说明
    Radioimmunoprecipitation
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    ELISA
    Use a concentration of 0.02 - 0.05 µg/ml.
    WB
    Use a concentration of 1 - 2 µg/ml.

    靶标

    • 相关性

      HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.

    • 细胞定位

      Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.

    • 数据库链接

      • 别名

        • p14 antibody
        • Protein Tat antibody
        • Transactivating regulatory protein antibody

      图片

      • Western blot - Anti-HIV1 tat antibody [1] (ab42359)
        Western blot - Anti-HIV1 tat antibody [1] (ab42359)
        Lane 1 : Anti-HIV1 tat antibody [1] (ab42359)


        Lane 1 : Recombinant HIV1 Tat (B subtype)
        Lane 2 : Protein size marker

      文献 (11)

      发表研究结果有使用 ab42359？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab42359 被引用在 11 文献中.

      • Lai M  et al. CRISPR/Cas9 Ablation of Integrated HIV-1 Accumulates Proviral DNA Circles with Reformed Long Terminal Repeats. J Virol 95:e0135821 (2021). PubMed: 34549986
      • Pereira LA  et al. MYB elongation is regulated by the nucleic acid binding of NF?B p50 to the intronic stem-loop region. PLoS One 10:e0122919 (2015). PubMed: 25853889
      • Vandergaast R  et al. Generation of West Nile virus infectious clones containing amino acid insertions between capsid and capsid anchor. Viruses 6:1637-53 (2014). WB . PubMed: 24721788
      • Lai MC  et al. Human DDX3 interacts with the HIV-1 Tat protein to facilitate viral mRNA translation. PLoS One 8:e68665 (2013). IP . PubMed: 23840900
      • Wright CA  et al. Effects of Tat proteins and Tat mutants of different human immunodeficiency virus type 1 clades on glial JC virus early and late gene transcription. J Gen Virol 94:514-23 (2013). PubMed: 23152365
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-4 of 4 Abreviews or Q&A

      Question

      I just wanted to know if  is also localized in the cytoplasm and not just in the cell nuclei.   Do you have any evidence for that?   In the literature I did find reports of Tat being expressed also in the cytoplasm. So I am investigating in that direction now.   My test sample is paraffin embedded tissue (lymphonoides from an infected and not treated HIV patient).    The staining if specific is fine then. Plenty of brown positive cells.   Thank you.  

      Read More
      Answer

      Thank you for your responce. I have been unable to find data showing HIV1 tat in the cytoplasm. I am sorry that I could not be of more help.  I would recommend using positive and negative controls to ensure that your results are accurate. I would like to direct you to the HPA link which has some excellent images. http://www.proteinatlas.org/ENSG00000102241 Please let me know if you have any questions or if there is anything else Abcam can do to help you meet your research goals.

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      Question

      Dear Abcam,   We are having unspecific staining with your tat antiboy.   We tested arrays of HIV negative tissues and we got membrane and cytoplamic staining not expected at all.   So the initial result  on lymphones taken from not  treated HIV patient was promising but when we tested it on negative control tissues as tonsils guts lymphonodes and liver we got the same staining result!   I look forward to hearing from you.   Best regards,  

      Read More
      Answer

      Thank you for contacting Abcam.

      I am sorry to hear of this issue with our product, especially after your earlier work seemed to have exciting implications. This product is very well mapped and I am surprised to hear of the results that you have seen you have tested in multiple negative (uninfected) control tissues in IHC-P.

      Would you be able to send me detailed information about your protocol? It would also be very helpful to see images of your stains. With this information I may be able to offer suggestions that may help or to offer a replacement or refund if we cannot resolve these issues.

      I look forward to your reply. Please let me know if you have any other questions.

      Read More

      Question

      Hi   if you have a look at this link:   http://www.uniprot.org/uniprot/P04610   you scroll down up to Subcellular location   Host nucleus › host nucleolus. Host cytoplasm. Secreted. Note: Probably localizes to both nuclear and nucleolar compartments. Nuclear localization is mediated through the interaction of the nuclear localization signal with importin KPNB1. Secretion occurs through a Golgi-independent pathway. Tat is released from infected cells to the extracellular space where it remains associated to the cell membrane, or is secreted into the cerebrospinal fluid and sera. Extracellular Tat can be endocytosed by surrounding uninfected cells via binding to several receptors depending on the cell type.   given that our lymphonodes test sample is from an HIV patient (not treated as well) that would explain why we see strong membrane staining.   What about?   Thank you.  

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      Answer

      Thank you for your reply. The information that you have sent and the tissue that you are staining on would seem to explain the membrane staining nicely. I certainly do appreciate your finding this for me and will add this information to our notes.  If you perform any staining on test samples of HIV infected but treated patients would you please let me know the results? Thanks again. If there is anything else that we can do for you, please do not hesitate to contact us.

      Read More

      Question

      Dear Abcam   We bought recently the anti tat antiboby  (ab42359) and I have a question for you:   we saw very good immunostaining on our test sample (HIV infected lymphonodes) but it was a cytoplasmic/membrane antigen stain   Your Ab data sheet says Cellular localization Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.   Any idea about? Is the staining specific?  

      Read More
      Answer

      Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I would like to gather further information regarding the samples tested and the protocol used to see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody. Could you please send me your protocol, including but not limited to:   Tissue preparation and fixation procedures. Any permeablization or antigen retrieval steps that you use. Blocking solution compositon, blocking time and temperature. Concentrations of the antibodies that you have tried, how long they were incubated and at what temperature. Any positive control tissues that you have used and what the results where of those. Any negative controls that you performed. Any images that you may have will be of great help as well. Thank you in advance for collecting this information for me.  If you have any questions please feel free to contact me. I look forward to receiving your reply.  

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