Anti-HIV1 Reverse Transcriptase抗体(ab63911)
Key features and details
- Rabbit polyclonal to HIV1 Reverse Transcriptase
- Suitable for: WB, ELISA
- Reacts with: Human immunodeficiency virus
- Isotype: IgG
概述
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产品名称
Anti-HIV1 Reverse Transcriptase抗体
参阅全部 HIV1 Reverse Transcriptase 一抗 -
描述
兔多克隆抗体to HIV1 Reverse Transcriptase -
宿主
Rabbit -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Human immunodeficiency virus -
免疫原
HIV1 Reverse Transcriptase, over-expressed as recombinant protein in E.coli
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阳性对照
- Extract of MT4 cells infected with HIV1 (LAI strain).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63911于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2500. Detects a band of approximately 51, 66 kDa.
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| ELISA |
Use at an assay dependent dilution.
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| 说明 |
|---|
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WB
1/2500. Detects a band of approximately 51, 66 kDa. |
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ELISA
Use at an assay dependent dilution. |
靶标
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相关性
HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied. -
数据库链接
- SwissProt: P03367 Human immunodeficiency virus
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别名
- HIV 1 Reverse transcriptase antibody
- HIV1 RT antibody
- Human Immunodeficiency virus 1 antibody
see all
图片
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Western blot - Anti-HIV1 Reverse Transcriptase antibody (ab63911)All lanes : Anti-HIV1 Reverse Transcriptase antibody (ab63911) at 1/2500 dilution
Lane 1 : Extract of MT4 cells
Lane 2 : Extract of MT4 cells infected with HIV-1 (LAI strain)
Observed band size: 51,66 kDa why is the actual band size different from the predicted? -
Western blot - Anti-HIV1 Reverse Transcriptase antibody (ab63911)
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab63911 被引用在 7 文献中.
- Benner BE et al. Perturbing HIV-1 Ribosomal Frameshifting Frequency Reveals a cis Preference for Gag-Pol Incorporation into Assembling Virions. J Virol 96:e0134921 (2022). PubMed: 34643428
- Imamichi T et al. Natural Occurring Polymorphisms in HIV-1 Integrase and RNase H Regulate Viral Release and Autoprocessing. J Virol 95:e0132321 (2021). PubMed: 34523971
- Slack RL et al. Conformational Changes in HIV-1 Reverse Transcriptase that Facilitate Its Maturation. Structure 27:1581-1593.e3 (2019). PubMed: 31471129
- Takahata T et al. Critical Contribution of Tyr15 in the HIV-1 Integrase (IN) in Facilitating IN Assembly and Nonenzymatic Function through the IN Precursor Form with Reverse Transcriptase. J Virol 91:N/A (2017). PubMed: 27795445
- Garcia-Miranda P et al. Stability of HIV Frameshift Site RNA Correlates with Frameshift Efficiency and Decreased Virus Infectivity. J Virol 90:6906-17 (2016). PubMed: 27194769
- Leng J et al. A cell-intrinsic inhibitor of HIV-1 reverse transcription in CD4(+) T cells from elite controllers. Cell Host Microbe 15:717-728 (2014). PubMed: 24922574
- Contreras X et al. Protein kinase C-delta regulates HIV-1 replication at an early post-entry step in macrophages. Retrovirology 9:37 (2012). WB . PubMed: 22554282