Anti-HIV1 Rev抗体[Rev-6] (ab85529)
![Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529)](https://www.abcam.cn/ps/products/85/ab85529/Images/ab85529-100399-anti-hiv1-rev-antibody-rev-6-western-blot.jpg "Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529)")
Key features and details
- Mouse monoclonal [Rev-6] to HIV1 Rev
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-HIV1 Rev抗体[Rev-6]
参阅全部 HIV1 Rev 一抗 -
描述
小鼠单克隆抗体[Rev-6] to HIV1 Rev -
宿主
Mouse -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
HIV1 Rev recombinant protein (clade B, HXB-3 isolate) with an N terminal His tag, expressed in bacteria.
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阳性对照
- WB: HeLa cells transfected with HIV1
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 2.94% Sodium citrate, 0.05% Glycerol, 0.00061% Tris -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from supernatants of hybridoma cell cultures by affinity chromatography based on Protein A. -
克隆
单克隆 -
克隆编号
Rev-6 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85529于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/250 - 1/500. Predicted molecular weight: 13 kDa.
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| ELISA |
1/100 - 1/200.
|
| 说明 |
|---|
|
WB
1/250 - 1/500. Predicted molecular weight: 13 kDa. |
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ELISA
1/100 - 1/200. |
靶标
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相关性
HIV1 expresses the trans-activator protein Rev which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. The HIV1 Rev protein as viral protein is expressed early in the virus life cycle and thus may be important targets for the immune control of HIV1 infection. -
别名
- Human immunodeficiency virus 1 Rev antibody
- Protein Rev antibody
- Regulator of expression of viral proteins antibody
- Rev antibody
图片
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Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529)Image from Wong R et al., Retrovirology. 2011 Jun 17;8:47.Fig 8.; doi:10.1186/1742-4690-8-47; 17 June, 2011, Retrovirology, 8:47Representative western blot detecting HIV1 Rev using ab85529 at 1/250 dilution. An HRP-conjugated secondary antibody (1/5000) was used.
HeLa cells stably transduced with an inducible Tet-On HIV-1 system, were untreated or treated for 4-5 h with TG009 or chlorhexidine, followed by induction of expression of the endogenous HIV-1 provirus by addition of doxycycline.
数据表及文件
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Datasheet download
文献 (9)
ab85529 被引用在 9 文献中.
- Broussau S et al. Packaging cells for lentiviral vectors generated using the cumate and coumermycin gene induction systems and nanowell single-cell cloning. Mol Ther Methods Clin Dev 29:40-57 (2023). PubMed: 36936448
- Lu W et al. The Polar Region of the HIV-1 Envelope Protein Determines Viral Fusion and Infectivity by Stabilizing the gp120-gp41 Association. J Virol 93:N/A (2019). PubMed: 30651369
- Kyei GB et al. Splicing Factor 3B Subunit 1 Interacts with HIV Tat and Plays a Role in Viral Transcription and Reactivation from Latency. MBio 9:N/A (2018). PubMed: 30401776
- Behrens RT et al. Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export. J Virol 91:N/A (2017). PubMed: 27852860
- Cochrane A HIV-1 Rev function and RNA nuclear-cytoplasmic export. Methods Mol Biol 1087:103-14 (2014). PubMed: 24158817
- Wong RW et al. Digoxin suppresses HIV-1 replication by altering viral RNA processing. PLoS Pathog 9:e1003241 (2013). WB . PubMed: 23555254
- Wong RW et al. Characterization of novel inhibitors of HIV-1 replication that function via alteration of viral RNA processing and rev function. Nucleic Acids Res 41:9471-83 (2013). Human . PubMed: 23945945
- Lund N et al. Differential effects of hnRNP D/AUF1 isoforms on HIV-1 gene expression. Nucleic Acids Res 40:3663-75 (2012). WB . PubMed: 22187150
- Wong R et al. Differential effect of CLK SR Kinases on HIV-1 gene expression: potential novel targets for therapy. Retrovirology 8:47 (2011). WB ; Human . PubMed: 21682887
