Anti-HIV1 p55 + p24 + p17抗体(ab63917)
Key features and details
- Rabbit polyclonal to HIV1 p55 + p24 + p17
- Suitable for: WB, ELISA
- Reacts with: Human immunodeficiency virus
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-HIV1 p55 + p24 + p17抗体 -
描述
兔多克隆抗体to HIV1 p55 + p24 + p17 -
宿主
Rabbit -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Human immunodeficiency virus -
免疫原
Recombinant protein p55 over-expressed in E.coli using a plasmid carrying the Gag p55 coding region of HIV1 virus.
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阳性对照
- Extract of MT4 cells infected with HIV1 (LAI strain); HeLa whole cell lysate transfected with a plasmid expressing HIV1 gag.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63917于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/2000. Detects a band of approximately 17,24,55 kDa.
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| ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/2000. Detects a band of approximately 17,24,55 kDa. |
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ELISA
Use at an assay dependent concentration. |
靶标
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相关性
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. p55 is digested by HIV1 protease into intermediate products p41 and p15. p41 is further digested into matrix protein p17 and capsid protein p24. Likewise, p15 is further digested into nucleocapsid protein p7 and to p6 and p1. -
数据库链接
- Entrez Gene: 155030 Human immunodeficiency virus
- SwissProt: P05888 Human immunodeficiency virus
- SwissProt: Q70622 Human immunodeficiency virus
- SwissProt: Q79665 Human immunodeficiency virus
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别名
- CA antibody
- Capsid protein p24 antibody
- Gag antibody
see all
图片
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Western blot - Anti-HIV1 p55 + p24 + p17 antibody (ab63917)All lanes : Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution
Lane 1 : Extract of MT4 cells
Lane 2 : Extract of MT4 cells infected with HIV1
(LAI strain)
Observed band size: 17,24,55 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 45 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-HIV1 p55 + p24 + p17 antibody (ab63917)This image is courtesy of an Abreview submitted by David Carpentier.All lanes : Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate transfected with a plasmid expressing HIV1 gag
Lane 2 : Untransfected HeLa whole cell lysate
Secondary
All lanes : Goat anti-rabbit IgG (whole molecule) HRP polymer at 1/50000 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (38)
ab63917 被引用在 38 文献中.
- Segura J et al. HIV-1 release requires Nef-induced caspase activation. PLoS One 18:e0281087 (2023). PubMed: 36780482
- Wang C et al. Antiviral Properties of HIV-1 Capsid Inhibitor GSK878. Antimicrob Agents Chemother 67:e0169422 (2023). PubMed: 37039636
- Shen W et al. Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein. Virol Sin 38:448-458 (2023). PubMed: 37172824
- Alves JM et al. Adjuvant effect of dendritic cells activator Imiquimod in genetic immunization with HIV-1 p55 Gag. J Immunol Tech Infect Dis 12:N/A (2023). PubMed: 37205236
- Gu F et al. HIV-1 promotes ubiquitination of the amyloidogenic C-terminal fragment of APP to support viral replication. Nat Commun 14:4227 (2023). PubMed: 37454116