Anti-HIV1 Nef抗体(ab63919)
Key features and details
- Guinea pig polyclonal to HIV1 Nef
- Suitable for: WB, ELISA
- Reacts with: Human immunodeficiency virus
- Isotype: IgG
概述
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产品名称
Anti-HIV1 Nef抗体
参阅全部 HIV1 Nef 一抗 -
描述
豚鼠多克隆抗体to HIV1 Nef -
宿主
Guinea pig -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Human immunodeficiency virus -
免疫原
Recombinant Nef protein over-expressed in E. coli with a plasmid carrying the Nef coding region of HIV1 virus, subtype B.
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阳性对照
- Extract of MT4 cells uninfected or infected with HIV1 (LAI strain).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
纯化说明
ab93919 is highly purified by several steps of chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63919于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 24 kDa.
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| ELISA |
Use at an assay dependent dilution.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 24 kDa. |
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ELISA
Use at an assay dependent dilution. |
靶标
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相关性
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
数据库链接
- Entrez Gene: 156110 Human immunodeficiency virus
- SwissProt: P03407 Human immunodeficiency virus
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别名
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
图片
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Western blot - Anti-HIV1 Nef antibody (ab63919)All lanes : Anti-HIV1 Nef antibody (ab63919) at 1/1000 dilution
Lane 1 : Extract of MT4 cells
Lane 2 : Extract of MT4 cells infected with HIV1 (LAI strain)
Predicted band size: 24 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (0)
ab63919 尚未被引用在任何文献中。
