Anti-HIV1 Nef抗体[3D12] (ab42355)
![Western blot - Anti-HIV1 Nef antibody [3D12] (ab42355)](https://www.abcam.cn/ps/products/42/ab42355/Images/ab42355-172785-anti-hiv1-nef-antibody-3d12-western-blot.jpg "Western blot - Anti-HIV1 Nef antibody [3D12] (ab42355)")
Key features and details
- Mouse monoclonal [3D12] to HIV1 Nef
- Suitable for: ICC/IF, ELISA, WB
- Reacts with: Human immunodeficiency virus
- Isotype: IgG1
概述
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产品名称
Anti-HIV1 Nef抗体[3D12]
参阅全部 HIV1 Nef 一抗 -
描述
小鼠单克隆抗体[3D12] to HIV1 Nef -
宿主
Mouse -
特异性
This antibody reacts with the rNEF of HIV 1 subtype B and C, -
经测试应用
适用于: ICC/IF, ELISA, WBmore details -
种属反应性
与反应: Human immunodeficiency virus -
免疫原
Recombinant Nef protein of HIV1 HXB2.
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表位
Mapped to amino acids 35-50 (RDLEKHGAITSSNTAA - HIV1 HXB2) -
常规说明
Also known as clone 01-002.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified by affinty chromatography. -
克隆
单克隆 -
克隆编号
3D12 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab42355于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| ELISA |
1/5000.
|
|
| WB |
1/500. Predicted molecular weight: 24 kDa.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
ELISA
1/5000. |
|
WB
1/500. Predicted molecular weight: 24 kDa. |
靶标
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相关性
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
数据库链接
- Entrez Gene: 156110 Human immunodeficiency virus
- SwissProt: P03407 Human immunodeficiency virus
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别名
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
图片
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Western blot - Anti-HIV1 Nef antibody [3D12] (ab42355)Lane 1 : Anti-HIV1 Nef antibody [3D12] (ab42355)
Lane 1 : Recombinant HIV1 Nef (B subtype)
Lane 2 : Protein size marker
Predicted band size: 24 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab42355 被引用在 19 文献中.
- Pushkarsky T et al. Abundance of Nef and p-Tau217 in Brains of Individuals Diagnosed with HIV-Associated Neurocognitive Disorders Correlate with Disease Severance. Mol Neurobiol 59:1088-1097 (2022). PubMed: 34843091
- Ye F et al. Recruitment of the CoREST transcription repressor complexes by Nerve Growth factor IB-like receptor (Nurr1/NR4A2) mediates silencing of HIV in microglial cells. PLoS Pathog 18:e1010110 (2022). PubMed: 35797416
- Duggan MR et al. Neuromodulation of BAG co-chaperones by HIV-1 viral proteins and H2O2: implications for HIV-associated neurological disorders. Cell Death Discov 7:60 (2021). PubMed: 33771978
- Ciccosanti F et al. High Levels of TRIM5a Are Associated with Xenophagy in HIV-1-Infected Long-Term Nonprogressors. Cells 10:N/A (2021). PubMed: 34069225
- Kumar P et al. HIV-1 Nef physically associate with CAMKIId - ASK-1 complex to inhibit p38MAPK signalling and apoptosis in infected cells. Life Sci 224:263-273 (2019). PubMed: 30902545