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AB66645

Anti-HIV1 integrase 抗体 [IN-2]

Anti-HIV1 integrase antibody [IN-2]

4

(3 Reviews)

|

(12 Publications)

Mouse Monoclonal POL antibody. Suitable for WB, IP, ICC/IF and reacts with Human immunodeficiency virus samples. Cited in 12 publications.

查看别名

HIV, Human Immunodeficiency Virus 1, IN, Integrase

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-HIV1 integrase antibody [IN-2] (AB66645)
  • ICC/IF

AbReview61938****

Immunocytochemistry/ Immunofluorescence - Anti-HIV1 integrase antibody [IN-2] (AB66645)

ab66645 staining HIV1 integrase in transfected 293T by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1 : 1), permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in PBS + 1% BSA) for 1 hour at 21°C. An Alexa Fluor® 555-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody (1/2000).

This image is courtesy of an anonymous Abreview

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

IN-2

亚型

IgG1

轻链类型

kappa

不含载体蛋白

No

反应种属

Human immunodeficiency virus

应用

IP, WB, ICC/IF

applications

特异性

ab66645 is specific for bacterial HIV1 integrase (IN) protein.

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搭配使用产品

Primary Antibodies

AB308072

Anti-HIV1 p55 + p24 antibody [EPR27327-79]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (AB308072)

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  • 0
Primary Antibodies

AB63911

Anti-HIV1 Reverse Transcriptase antibody

Western blot - Anti-HIV1 Reverse Transcriptase antibody (AB63911)

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  • 0
Primary Antibodies

AB183497

Anti-VSV-G tag antibody [EPR12997]

RabMAb|Recombinant|Lab Essentials

Western blot - Anti-VSV-G tag antibody [EPR12997] (AB183497)

  • 4
  • 2
Primary Antibodies

AB32352

Anti-HIV1 p24 antibody [P131]

RabMAb|Recombinant

Western blot - Anti-HIV1 p24 antibody [P131] (AB32352)

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  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human immunodeficiency virus": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
存储溶液
Preservative: 0.1% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HIV-1 integrase also known simply as integrase is an enzyme that acts mechanically by catalyzing the insertion of viral DNA into the host DNA. It is essential for the replication cycle of HIV-1 the virus responsible for AIDS. The molecular mass of the integrase protein is approximately 32 kDa. Expression of integrase occurs within the viral particle and is integral to the viral genome. Integrases allow HIV-1 to integrate its genetic material into the DNA of host cells a vital step for viral replication.
Biological function summary

HIV-1 integrase operates as a part of the pre-integration complex (PIC) within infected host cells. This enzyme facilitates two consecutive reactions: 3'-end processing and strand transfer. The 3'-end processing involves trimming the ends of viral DNA and strand transfer involves the integration of viral DNA into host genomic DNA. The activity of integrase needs to be coordinated with other viral proteins such as reverse transcriptase to ensure successful integration and replication cycles.

Pathways

Integrase is an important component in the retroviral integration pathway which is important for the proliferation of HIV-1 within the host. This pathway includes steps that involve other viral proteins such as reverse transcriptase and protease. Reverse transcriptase synthesizes viral DNA from RNA which integrase then incorporates into the host DNA. The integrase pathway interplays with host cellular mechanisms and manipulates them to benefit viral replication.

HIV-1 integrase is associated with the progression of HIV/AIDS. Inhibiting this protein effectively blocks the integration step preventing new infections of host cells and is an established therapeutic approach in antiretroviral treatments. Integrase inhibitors such as raltegravir specifically target this enzyme illustrating its pivotal role in disease management for HIV/AIDS patients. By targeting HIV integrase treatment regimens aim to disrupt the life cycle of HIV-1 and suppress viral loads in patients.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information HIV1 integrase
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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 95:e0132321 PubMed34523971

2021

Natural Occurring Polymorphisms in HIV-1 Integrase and RNase H Regulate Viral Release and Autoprocessing.

Applications

Unspecified application

Species

Unspecified reactive species

Tomozumi Imamichi,John G Bernbaum,Sylvain Laverdure,Jun Yang,Qian Chen,Helene Highbarger,Ming Hao,Hongyan Sui,Robin Dewar,Weizhong Chang,H Clifford Lane

Antimicrobial agents and chemotherapy 65:e0103921 PubMed34228546

2021

A Small Molecule, ACAi-028, with Anti-HIV-1 Activity Targets a Novel Hydrophobic Pocket on HIV-1 Capsid.

Applications

Unspecified application

Species

Unspecified reactive species

Travis Chia,Tomofumi Nakamura,Masayuki Amano,Nobutoki Takamune,Masao Matsuoka,Hirotomo Nakata

PLoS pathogens 16:e1009147 PubMed33351861

2020

Mutations altering acetylated residues in the CTD of HIV-1 integrase cause defects in proviral transcription at early times after integration of viral DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Shelby Winans,Stephen P Goff

Journal of virology 94: PubMed32611758

2020

A Conformational Escape Reaction of HIV-1 against an Allosteric Integrase Inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Tomofumi Nakamura,Teruya Nakamura,Masayuki Amano,Toshikazu Miyakawa,Yuriko Yamagata,Masao Matsuoka,Hirotomo Nakata

Cell reports 32:107887 PubMed32668246

2020

Multiomics Investigation Revealing the Characteristics of HIV-1-Infected Cells In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Hirofumi Aso,Shumpei Nagaoka,Eiryo Kawakami,Jumpei Ito,Saiful Islam,Benjy Jek Yang Tan,Shinji Nakaoka,Koichi Ashizaki,Katsuyuki Shiroguchi,Yutaka Suzuki,Yorifumi Satou,Yoshio Koyanagi,Kei Sato

Journal of virology 94: PubMed31941774

2020

Capsid-Labelled HIV To Investigate the Role of Capsid during Nuclear Import and Integration.

Applications

Unspecified application

Species

Unspecified reactive species

Irena Zurnic Bönisch,Lieve Dirix,Veerle Lemmens,Doortje Borrenberghs,Flore De Wit,Frank Vernaillen,Susana Rocha,Frauke Christ,Jelle Hendrix,Johan Hofkens,Zeger Debyser

The Journal of biological chemistry 292:19814-19825 PubMed28972144

2017

Resistance to pyridine-based inhibitor KF116 reveals an unexpected role of integrase in HIV-1 Gag-Pol polyprotein proteolytic processing.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley C Hoyte,Augusta V Jamin,Pratibha C Koneru,Matthew J Kobe,Ross C Larue,James R Fuchs,Alan N Engelman,Mamuka Kvaratskhelia

Journal of virology 91: PubMed27795445

2016

Critical Contribution of Tyr15 in the HIV-1 Integrase (IN) in Facilitating IN Assembly and Nonenzymatic Function through the IN Precursor Form with Reverse Transcriptase.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuro Takahata,Eri Takeda,Minoru Tobiume,Kenzo Tokunaga,Masaru Yokoyama,Yu-Lun Huang,Atsuhiko Hasegawa,Tatsuo Shioda,Hironori Sato,Mari Kannagi,Takao Masuda

Retrovirology 13:49 PubMed27459960

2016

HIV integration and the establishment of latency in CCL19-treated resting CD4(+) T cells require activation of NF-κB.

Applications

IP

Species

Human

Suha Saleh,Hao K Lu,Vanessa Evans,David Harisson,Jingling Zhou,Anthony Jaworowski,Georgina Sallmann,Karey Y Cheong,Talia M Mota,Surekha Tennakoon,Thomas A Angelovich,Jenny Anderson,Andrew Harman,Anthony Cunningham,Lachlan Gray,Melissa Churchill,Johnson Mak,Heidi Drummer,Dimitrios N Vatakis,Sharon R Lewin,Paul U Cameron

Retrovirology 12:16 PubMed25809198

2015

HIV-1 IN/Pol recruits LEDGF/p75 into viral particles.

Applications

Unspecified application

Species

Unspecified reactive species

Belete Ayele Desimmie,Caroline Weydert,Rik Schrijvers,Sofie Vets,Jonas Demeulemeester,Paul Proost,Igor Paron,Jan De Rijck,Jan Mast,Norbert Bannert,Rik Gijsbers,Frauke Christ,Zeger Debyser
View all publications
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