Anti-Histone H4 (tri methyl K20) 抗体 - ChIP Grade

• WB

Project

Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (AB9053)

ab9053 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 5 where the activity of ab9053 is specifically blocked by the addition of the immunizing peptide (ab17567).

All lanes:

Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053) at 1 µg/mL

Lanes 1, 5, 6 and 7:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Human Histone H4 peptide (<a href='/products/unavailable/human-histone-h4-peptide-ab2622'>ab2622</a>)

Lane 3:

Calf thymus histone lysate with Human Histone H4 (mono methyl K20) peptide (<a href='/products/unavailable/human-histone-h4-mono-methyl-k20-peptide-ab17043'>ab17043</a>)

Lane 4:

Calf thymus histone lysate with Human Histone H4 (di methyl K20) peptide (<a href='/products/unavailable/human-histone-h4-di-methyl-k20-peptide-ab14964'>ab14964</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

false

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (AB9053)

IHC image of Histone H4 (tri methyl K20) staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9053, 0.2μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

AbReview45378****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (AB9053)

Immunohistochemical staining of paraffin embedded, PFA fixed, mouse liver using ab9053 at 1/100. Heat mediated antigen retrieval was performed using sodium citrate buffer, pH 6, and the sample was permeabilized with 0.05% Triton X 100. The sample was blocked in 5% BSA for 1 hour at room temperature and was then incubated with primary antibody for 3 hours in PBS/0.05% Triton X 100 at room temperature. An Alexa Fluor^® 555 donkey anyti-rabbit was used as the secondary at 1/250.

This image is courtesy of an anonymous Abreview.

• ChIP

Unknown

ChIP - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (AB9053)

Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 6 μl of ab9053 (blue), and 20 μl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

• WB

Project

Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (AB9053)

ab9053 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 5 where the activity of ab9053 is specifically blocked by the addition of the immunizing peptide (ab17567).

All lanes:

Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053) at 1 µg/mL

Lanes 1, 5, 6 and 7:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Human Histone H4 peptide (<a href='/products/unavailable/human-histone-h4-peptide-ab2622'>ab2622</a>)

Lane 3:

Calf thymus histone lysate with Human Histone H4 (mono methyl K20) peptide (<a href='/products/unavailable/human-histone-h4-mono-methyl-k20-peptide-ab17043'>ab17043</a>)

Lane 4:

Calf thymus histone lysate with Human Histone H4 (di methyl K20) peptide (<a href='/products/unavailable/human-histone-h4-di-methyl-k20-peptide-ab14964'>ab14964</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

false

• WB

Unknown

Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (AB9053)

Left panel : a fission yeast whole cell extract and recombinant (r) fission yeast histone H4.

Right panel : fission yeast whole cell extracts from strains with only a single wt (h4) or mutant (h4K20R) gene were immunoblotted.

Review by Steven Sanders submitted 13 August 2004

All lanes:

Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)

Predicted band size: 11 kDa

false