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Epigenetics and Nuclear Signaling Histones H2A Methylated

Anti-Histone H4 (symmetric di methyl R3)抗体(ab5823)

  • Datasheet
  • SDS
Reviews (20)Q&A (5)References (93)

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Western blot - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)
  • Peptide Array - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)

Key features and details

  • Rabbit polyclonal to Histone H4 (symmetric di methyl R3)
  • Suitable for: IHC-P, PepArr, WB
  • Reacts with: Cow, Human, Arabidopsis thaliana
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Histone H4 (symmetric di methyl R3)抗体
    参阅全部 Histone H4 一抗
  • 描述

    兔多克隆抗体to Histone H4 (symmetric di methyl R3)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-P, PepArr, WBmore details
  • 种属反应性

    与反应: Cow, Human, Arabidopsis thaliana
    预测可用于: Mouse, Rat, Pig, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Tobacco, Rainbow trout, Corn
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab14791)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Histones
    • H2A
    • Methylated
    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Methylated
    • Epigenetics and Nuclear Signaling
    • Histones
    • H2A and H4

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Histone H4 protein (ab198115)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)
    • Human Histone H4 peptide (ab13843)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5823于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (3)
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
PepArr
Use a concentration of 0.2 - 0.02 µg/ml.
WB (7)
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11.5 kDa).
说明
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
PepArr
Use a concentration of 0.2 - 0.02 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11.5 kDa).

靶标

  • 功能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 序列相似性

    Belongs to the histone H4 family.
  • 翻译后修饰

    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • 细胞定位

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P62805 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 318846 Drosophila melanogaster
    • Entrez Gene: 3771854 Drosophila melanogaster
    • Entrez Gene: 3771893 Drosophila melanogaster
    • Entrez Gene: 3771908 Drosophila melanogaster
    • Entrez Gene: 3771935 Drosophila melanogaster
    • Entrez Gene: 3771938 Drosophila melanogaster
    • Entrez Gene: 3771941 Drosophila melanogaster
    • Entrez Gene: 3771947 Drosophila melanogaster
    • Entrez Gene: 3772096 Drosophila melanogaster
    • Entrez Gene: 3772113 Drosophila melanogaster
    • Entrez Gene: 3772129 Drosophila melanogaster
    • Entrez Gene: 3772172 Drosophila melanogaster
    • Entrez Gene: 3772211 Drosophila melanogaster
    • Entrez Gene: 3772254 Drosophila melanogaster
    • Entrez Gene: 3772314 Drosophila melanogaster
    • Entrez Gene: 3772317 Drosophila melanogaster
    • Entrez Gene: 3772319 Drosophila melanogaster
    • Entrez Gene: 3772325 Drosophila melanogaster
    • Entrez Gene: 3772509 Drosophila melanogaster
    • Entrez Gene: 3772519 Drosophila melanogaster
    • Entrez Gene: 3772666 Drosophila melanogaster
    • Entrez Gene: 3772708 Drosophila melanogaster
    • Entrez Gene: 41773 Drosophila melanogaster
    • Entrez Gene: 121504 Human
    • Entrez Gene: 554313 Human
    • Entrez Gene: 8294 Human
    • Entrez Gene: 8359 Human
    • Entrez Gene: 8360 Human
    • Entrez Gene: 8361 Human
    • Entrez Gene: 8362 Human
    • Entrez Gene: 8363 Human
    • Entrez Gene: 8364 Human
    • Entrez Gene: 8365 Human
    • Entrez Gene: 8366 Human
    • Entrez Gene: 8367 Human
    • Entrez Gene: 8368 Human
    • Entrez Gene: 8370 Human
    • Entrez Gene: 100041230 Mouse
    • Entrez Gene: 319155 Mouse
    • Entrez Gene: 319156 Mouse
    • Entrez Gene: 319157 Mouse
    • Entrez Gene: 319158 Mouse
    • Entrez Gene: 319159 Mouse
    • Entrez Gene: 319160 Mouse
    • Entrez Gene: 319161 Mouse
    • Entrez Gene: 320332 Mouse
    • Entrez Gene: 326619 Mouse
    • Entrez Gene: 326620 Mouse
    • Entrez Gene: 674678 Mouse
    • Entrez Gene: 69386 Mouse
    • Entrez Gene: 97122 Mouse
    • Entrez Gene: 64627 Rat
    • Entrez Gene: 447787 Xenopus laevis
    • Omim: 142750 Human
    • Omim: 602822 Human
    • Omim: 602823 Human
    • Omim: 602824 Human
    • Omim: 602825 Human
    • Omim: 602826 Human
    • Omim: 602827 Human
    • Omim: 602828 Human
    • Omim: 602829 Human
    • Omim: 602830 Human
    • Omim: 602831 Human
    • Omim: 602833 Human
    • SwissProt: P84040 Drosophila melanogaster
    • SwissProt: P62805 Human
    • SwissProt: P62806 Mouse
    • SwissProt: P62804 Rat
    • SwissProt: P02309 Saccharomyces cerevisiae
    • SwissProt: P62799 Xenopus laevis
    • Unigene: 21500 Drosophila melanogaster
    • Unigene: 29514 Drosophila melanogaster
    • Unigene: 29527 Drosophila melanogaster
    • Unigene: 30219 Drosophila melanogaster
    • Unigene: 30220 Drosophila melanogaster
    • Unigene: 30221 Drosophila melanogaster
    • Unigene: 30223 Drosophila melanogaster
    • Unigene: 30868 Drosophila melanogaster
    • Unigene: 30869 Drosophila melanogaster
    • Unigene: 30871 Drosophila melanogaster
    • Unigene: 30872 Drosophila melanogaster
    • Unigene: 30873 Drosophila melanogaster
    • Unigene: 30876 Drosophila melanogaster
    • Unigene: 33873 Drosophila melanogaster
    • Unigene: 5747 Drosophila melanogaster
    • Unigene: 143080 Human
    • Unigene: 247816 Human
    • Unigene: 248172 Human
    • Unigene: 248178 Human
    • Unigene: 248179 Human
    • Unigene: 278483 Human
    • Unigene: 46423 Human
    • Unigene: 528055 Human
    • Unigene: 533295 Human
    • Unigene: 55468 Human
    • Unigene: 591790 Human
    • Unigene: 655235 Human
    • Unigene: 662174 Human
    • Unigene: 706635 Human
    • Unigene: 718935 Human
    • Unigene: 144300 Mouse
    • Unigene: 14775 Mouse
    • Unigene: 158272 Mouse
    • Unigene: 227295 Mouse
    • Unigene: 228709 Mouse
    • Unigene: 246720 Mouse
    • Unigene: 255646 Mouse
    • Unigene: 260530 Mouse
    • Unigene: 261642 Mouse
    • Unigene: 261662 Mouse
    • Unigene: 261664 Mouse
    • Unigene: 377875 Mouse
    • Unigene: 442307 Mouse
    • Unigene: 85592 Xenopus laevis
    see all
  • 别名

    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4.k antibody
    • H4/a antibody
    • H4/b antibody
    • H4/c antibody
    • H4/d antibody
    • H4/e antibody
    • H4/g antibody
    • H4/h antibody
    • H4/I antibody
    • H4/j antibody
    • H4/k antibody
    • H4/m antibody
    • H4/n antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • H4FM antibody
    • H4FN antibody
    • H4M antibody
    • HIST1H4A antibody
    • HIST1H4B antibody
    • HIST1H4C antibody
    • HIST1H4D antibody
    • HIST1H4E antibody
    • HIST1H4F antibody
    • HIST1H4H antibody
    • HIST1H4I antibody
    • HIST1H4J antibody
    • HIST1H4K antibody
    • HIST1H4L antibody
    • HIST2H4 antibody
    • HIST2H4A antibody
    • Hist4h4 antibody
    • Histone 1 H4a antibody
    • Histone 1 H4b antibody
    • Histone 1 H4c antibody
    • Histone 1 H4d antibody
    • Histone 1 H4e antibody
    • Histone 1 H4f antibody
    • Histone 1 H4h antibody
    • Histone 1 H4i antibody
    • Histone 1 H4j antibody
    • Histone 1 H4k antibody
    • Histone 1 H4l antibody
    • Histone 2 H4a antibody
    • histone 4 H4 antibody
    • Histone H4 antibody
    • MGC24116 antibody
    see all

图片

  • Western blot - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)
    Western blot - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)
    Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823) at 1 µg + Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

    Secondary
    VHH Single Domain Anti-Rabbit IgG Fc (HRP) (ab191866) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11.5 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab5823 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)


    IHC image of ab5823 staining Histone H4 (symmetric di methyl R3) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5823, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Peptide Array - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)
    Peptide Array - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)

    All batches of ab5823 are tested in Peptide Array against peptides to different Histone H4 and H2A modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H4 - symmetric di methyl R3 peptide (ab14791), indicating that this antibody specifically recognises the Histone H4 - symmetric di methyl R3 modification.

    ab14791 - Histone H4 - symmetric di methyl R3

    ab17416 - Histone H4 asymmetric di methyl R3

    ab15821 - Histone H4 - unmodified

    ab22399 - Histone H2A symmetric di methyl R3

    ab22398 - Histone H2A asymmetric di methyl R3

    ab13186 - Histone H2A - unmodified

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (93)

发表研究结果有使用 ab5823?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5823 被引用在 93 文献中.

  • Yang T  et al. The PRMT6/PARP1/CRL4B Complex Regulates the Circadian Clock and Promotes Breast Tumorigenesis. Adv Sci (Weinh) 10:e2202737 (2023). PubMed: 36941223
  • Drozda A  et al. Nitric Oxide Implication in Potato Immunity to Phytophthora infestans via Modifications of Histone H3/H4 Methylation Patterns on Defense Genes. Int J Mol Sci 23:N/A (2022). PubMed: 35409411
  • Yao B  et al. The circSPON2/miR-331-3p axis regulates PRMT5, an epigenetic regulator of CAMK2N1 transcription and prostate cancer progression. Mol Cancer 21:119 (2022). PubMed: 35624451
  • Zhang J  et al. The PRMT5-LSD1 axis confers Slug dual transcriptional activities and promotes breast cancer progression. J Exp Clin Cancer Res 41:191 (2022). PubMed: 35655230
  • Lee J  et al. PRMT1 is required for the generation of MHC-associated microglia and remyelination in the central nervous system. Life Sci Alliance 5:N/A (2022). PubMed: 35705491
View all Publications for this product

客户评价及客户问答

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1-10 of 25 Abreviews or Q&A

ChIP abreview for Anti-Histone H4 (symmetric di methyl R3) antibody

Poor
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - nuclear (mouse CD4 T cells)
Negative control
IgG
Specification
mouse CD4 T cells
Detection step
Other
Type
Native ChIP (N-ChIP)
Positive control
H3K4me3
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 11 2024

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H4 (symmetric di methyl R3) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse lemur Cell (human umbilical vein endothelial cell)
Permeabilization
Yes - 0.5% TritonX-100
Specification
human umbilical vein endothelial cell
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. XXXXXX XXXXXX

Verified customer

提交于 Aug 01 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H4 (symmetric di methyl R3) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Liver)
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 01 2017

ChIP abreview for Anti-Histone H4 (symmetric di methyl R3) antibody

Average
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - whole cell (embryonic stem cell)
Negative control
IgG
Specification
embryonic stem cell
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde (1%)
Positive control
H3K27me3 and H3K4me3
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Dan Stummer

Verified customer

提交于 Apr 18 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H4 (symmetric di methyl R3) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse embryonic fibroblasts)
Permeabilization
Yes - 0.3% Triton X-100
Specification
Mouse embryonic fibroblasts
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 28 2013

Western blot abreview for Anti-Histone H4 (symmetric di methyl R3) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human HeLa cells)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
Human HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 18 2013

Western blot abreview for Anti-Histone H4 (symmetric di methyl R3) antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Drosophila melanogaster Tissue lysate - nuclear (Fruit fly embryo)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
5 µg
Specification
Fruit fly embryo
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 18 2013

Western blot abreview for Anti-Histone H4 (symmetric di methyl R3) antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neuro2A- neuroblastoma)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
40 µg
Specification
Neuro2A- neuroblastoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 07 2013

Question

Hello, I am interested in acquiring this antibody (ab5823) for ChIP and IHC/IF studies with a mutant of mine but there is no information on whether this antibody works with Drosophila fruit flies. I was wondering if you might be able to BLAST the immunogenic sequence against Drosophila melanogaster and find out the percentage of alignment, so that we can gauge on whether or not to try the antibody. Thanks for your time.

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Abcam community

Verified customer

Asked on Jan 23 2013

Answer

Thank you for your enquiry.



I blasted the sequence and there is a 100% homology between the immunogen and the sequence for Histone H4 of Drosophila melanogaster.



Please let us know if you have further questions.

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Abcam Scientific Support

回复于 Jan 23 2013

Question

Dear ,
I am hoping to get the antibody to work on a western blot and/or immunohistochemistry on cells.
Many thanks,

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Abcam community

Verified customer

Asked on Nov 13 2012

Answer

DISCOUNT CODE: XXXXX


Expiration date: XXXXX


Value: £252.00



I am very pleased to hear you would like to accept our offer and test ab5823 in Chicken with WB. This code will give you £252.00 off your next order before the expiration date. To redeem this offer, please submit an Abreview for the new species and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

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Abcam Scientific Support

回复于 Nov 13 2012

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