重组Anti-Histone H4 (phospho S1)抗体[RM194] (ab222769)
![Western blot - Anti-Histone H4 (phospho S1) antibody [RM194] (ab222769)](https://www.abcam.cn/ps/products/222/ab222769/Images/ab222769-282772-anti-histone-h4-phospho-s1-antibody-rm194-western-blot.jpg "Western blot - Anti-Histone H4 (phospho S1) antibody [RM194] (ab222769)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM194] to Histone H4 (phospho S1)
- Suitable for: WB, ICC/IF
- Reacts with: Human
概述
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产品名称
Anti-Histone H4 (phospho S1)抗体[RM194]
参阅全部 Histone H4 一抗 -
描述
兔单克隆抗体[RM194] to Histone H4 (phospho S1) -
宿主
Rabbit -
特异性
ab222769 reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighboring Arginine 3 modifications. No cross reactivity with Histone H2A phosphorylated at Serine 1. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: a wide range of other species
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免疫原
Synthetic peptide corresponding to Histone H4 (phospho S1).
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阳性对照
- WB: HeLa whole cell lysate. ICC/IF: HeLa cells.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
RM194 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab222769于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 0.5 - 2 µg/ml.
|
| ICC/IF |
Use a concentration of 1 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. |
|
ICC/IF
Use a concentration of 1 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
靶标
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功能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
序列相似性
Belongs to the histone H4 family. -
翻译后修饰
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
别名
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
图片
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Western blot - Anti-Histone H4 (phospho S1) antibody [RM194] (ab222769)All lanes : Anti-Histone H4 (phospho S1) antibody [RM194] (ab222769) at 0.5 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Recombinant Histone H4 -
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (phospho S1) antibody [RM194] (ab222769)](https://www.abcam.cn/ps/products/222/ab222769/Images/ab222769-282776-anti-histone-h4-phospho-s1-antibody-rm194-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (phospho S1) antibody [RM194] (ab222769)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (red) using ab222769 (2 μg/mL) in ICC/IF.
Actin filaments were labeled with fluorescein phalloidin (green).
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![Anti-Histone H4 (phospho S1) antibody [RM194] (ab222769)](https://www.abcam.cn/ps/products/222/ab222769/Images/ab222769-3-benefits-of-recombinant-antibodies.png)
Anti-Histone H4 (phospho S1) antibody [RM194] (ab222769)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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