重组Anti-Histone H4 (mono methyl K77)抗体[EPR17921(2)] (ab177828)
![Western blot - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828)](https://www.abcam.cn/ps/products/177/ab177828/Images/ab177828-248709-anti-histone-h4-mono-methyl-k77-antibody-epr179212-western-blot.jpg "Western blot - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17921(2)] to Histone H4 (mono methyl K77)
- Suitable for: PepArr, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Histone H4 (mono methyl K77)抗体[EPR17921(2)]
参阅全部 Histone H4 一抗 -
描述
兔单克隆抗体[EPR17921(2)] to Histone H4 (mono methyl K77) -
宿主
Rabbit -
经测试应用
适用于: PepArr, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa and NIH/3T3 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17921(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177828于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| PepArr |
Use at an assay dependent concentration.
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| WB |
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
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| 说明 |
|---|
|
PepArr
Use at an assay dependent concentration. |
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WB
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
靶标
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功能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
序列相似性
Belongs to the histone H4 family. -
翻译后修饰
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
别名
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
图片
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Western blot - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828)Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% BSA/TBST.
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![Western blot - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828)](https://www.abcam.cn/ps/products/177/ab177828/Images/ab177828-248708-anti-histone-h4-mono-methyl-k77-antibody-epr179212-western-blot.jpg)
Western blot - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828)Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828) at 1/1000 dilution + NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% BSA/TBST.
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![Peptide Array - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828)](https://www.abcam.cn/ps/products/177/ab177828/Images/ab177828-248851-anti-histone-h4-mono-methyl-k77-antibody-epr179212-peptide-array.jpg)
Peptide Array - Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828)ab177828 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here. -
![Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828)](https://www.abcam.cn/ps/products/177/ab177828/Images/ab177828-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828)
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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