重组Anti-Histone H4 (methyl R67)抗体[EPR18914] (ab177827)
![Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)](https://www.abcam.cn/ps/products/177/ab177827/Images/ab177827-251471-anti-histone-h4-methyl-r67-antibody-epr18914-western-blot.jpg "Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18914] to Histone H4 (methyl R67)
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Histone H4 (methyl R67)抗体[EPR18914]
参阅全部 Histone H4 一抗 -
描述
兔单克隆抗体[EPR18914] to Histone H4 (methyl R67) -
宿主
Rabbit -
特异性
This antibody detects mono methyl, asymmetric di methyl and symmetric di methyl arginine 67. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human testis lysate; RAW 264.7, F9, NIH/3T3 and HeLa whole cell lysates; mouse spleen lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18914 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177827于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
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| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
靶标
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功能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
序列相似性
Belongs to the histone H4 family. -
翻译后修饰
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
别名
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
图片
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Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/5000 dilution + Human testis lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)](https://www.abcam.cn/ps/products/177/ab177827/Images/ab177827-251470-anti-histone-h4-methyl-r67-antibody-epr18914-western-blot.jpg)
Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)All lanes : Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/5000 dilution
Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)](https://www.abcam.cn/ps/products/177/ab177827/Images/ab177827-251469-anti-histone-h4-methyl-r67-antibody-epr18914-western-blot.jpg)
Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)All lanes : Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cells) whole cell lysate
Lane 3 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 11 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time:
Lane 1 & 2: 4 seconds; Lane 3: 15 seconds.
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![Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)](https://www.abcam.cn/ps/products/177/ab177827/Images/ab177827-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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