Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-Histone H4抗体[EPR16599] - ChIP Grade (ab177840)

Reviews (4) Submit a question References (22)
ChIP - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Peptide Array - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16599] to Histone H4 - ChIP Grade
  • Suitable for: PepArr, ChIP, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human, Drosophila melanogaster, Recombinant fragment

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free HRP

概述

  • 产品名称

    Anti-Histone H4抗体[EPR16599] - ChIP Grade
    参阅全部 Histone H4 一抗

  • 描述

    兔单克隆抗体[EPR16599] to Histone H4 - ChIP Grade

  • 宿主

    Rabbit

  • 经测试应用

    适用于: PepArr, ChIP, IHC-P, WB, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Human, Drosophila melanogaster, Recombinant fragment
    预测可用于: a wide range of other species

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa and NIH/3T3 whole cell lysates; Drosophila whole lysate. ICC/IF: HeLa cells. IHC-P: Human colon, mouse pancreas and rat cerebral cortex tissues. ChIP: Chromatin from HeLa cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR16599

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab177840于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
PepArr
Use at an assay dependent concentration.
ChIP
Use 2 µg for 25 µg of chromatin.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (4)
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

We recommend using 3% milk as the blocking agent for Western blot.
ICC/IF
1/100.
说明
PepArr
Use at an assay dependent concentration.
ChIP
Use 2 µg for 25 µg of chromatin.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

We recommend using 3% milk as the blocking agent for Western blot.
ICC/IF
1/100.

靶标

图片

  • ChIP - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    ChIP - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with 0.75% formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab177840 (blue), and 20μl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (SYBR approach). Primers are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    All lanes : Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840) at 1/1000 dilution

    Lanes 1 & 6 : Histone H1 Recombinant Protein
    Lanes 2 & 7 : Histone H2A Recombinant Protein
    Lanes 3 & 8 : Histone H2B Recombinant Protein
    Lanes 4 & 9 : Histone H3.1 Recombinant Protein
    Lanes 5 & 10 : Histone H4 Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (H+L), Peroxidase Conjugated at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    Lanes 1-5: 1% BSA blocking buffer

    Lanes 6-10: 3% Milk blocking buffer

     

    We recommend using 3% milk as the blocking agent for Western blot.

  • Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840) at 1/1000 dilution + Drosophila whole lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    All lanes : Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysates
    Lane 2 : NIH/3T3 whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Peptide Array - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Peptide Array - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    ab177840 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Histone H4 with ab177840 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab177840 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H4 with ab177840 at 1/2000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of Human colon tissue is observed. Counter stained with Hematoxylin. 

    Negative control: PBS instead of primary antibody; secondary antibody is prediluted Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Histone H4 with ab177840 at 1/2000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of mouse pancreas tissue is observed. Counter stained with Hematoxylin.

    Negative control: PBS instead of primary antibody; secondary antibody is prediluted Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Histone H4 with ab177840 at 1/2000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on neuron cells of cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Negative control: PBS instead of primary antibody; secondary antibody is prediluted Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

文献 (22)

发表研究结果有使用 ab177840？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab177840 被引用在 22 文献中.

  • Tirado-Class N  et al. DCAF14 regulates CDT2 to promote SET8-dependent replication fork protection. Life Sci Alliance 7:N/A (2024). PubMed: 37940188
  • Wang YC  et al. Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination. Cell Rep 42:112296 (2023). PubMed: 36961817
  • Mohammed Ismail W  et al. MacroH2A histone variants modulate enhancer activity to repress oncogenic programs and cellular reprogramming. Commun Biol 6:215 (2023). PubMed: 36823213
  • Qiu B  et al. KAT8 acetylation-controlled lipolysis affects the invasive and migratory potential of colorectal cancer cells. Cell Death Dis 14:164 (2023). PubMed: 36849520
  • Li M  et al. Epigenetic Control of Translation Checkpoint and Tumor Progression via RUVBL1-EEF1A1 Axis. Adv Sci (Weinh) 10:e2206584 (2023). PubMed: 37075745
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-Histone H4 antibody [EPR16599] - ChIP Grade

 Good
Abreviews
abreview image
Application
Western blot
Sample
Schmidtea mediterranea Tissue lysate - whole (whole worm)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
10 µg
Specification
whole worm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 12 2022

Western blot abreview for Anti-Histone H4 antibody [EPR16599] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
mouse embryonic stem cells (J1)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 02 2018

Western blot abreview for Anti-Histone H4 antibody [EPR16599] - ChIP Grade

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (MCF7 cells)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
10 µg
Treatment
20ng/ml TNF alpha
Specification
MCF7 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ifeoluwa Adewumi

Verified customer

提交于 Feb 14 2017

Western blot abreview for Anti-Histone H4 antibody [EPR16599] - ChIP Grade

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Recombinant protein (Recombinant octamers & purified K-562 cell histone)
Gel Running Conditions
Reduced Denaturing (18% gel)
Loading amount
0.5 µg
Specification
Recombinant octamers & purified K-562 cell histone
Blocking step
Odyssey blocking solution 1/1 (vol/vol) PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ragnhild Eskeland

Verified customer

提交于 May 11 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com