Anti-Histone H4抗体- ChIP Grade (ab7311)

Thanks in advance for filling out the questionnaire below for each antibody. The answers will help me to assist you most efficiently.

1) Abcam product code ab



2) Abcam order reference number or product batch number



3) Description of the problem



4) Sample preparation:

Type of sample (whole cell lysates, fraction, recombinant protein…):

Lysis buffer

Protease inhibitors:

Phosphatase inhibitors

Reducing agent

Boiling for ≥5 min? yes/no

Protein loaded ug/lane or cells/lane

Positive control

Negative control



5) Percentage of gel

Type of membrane

Protein transfer verified

Blocking agent and concentration

Blocking time

Blocking temperature



6) Primary antibody (If more than one was used, describe in “additional notes”) :

Concentration or dilution

Diluent buffer

Incubation time

Incubation temperature:



7) Secondary antibody:

Species:

Reacts against:

Concentration or dilution

Diluent buffer

Incubation time

Incubation temperature:

Fluorochrome or enzyme conjugate:



8) Washing after primary and secondary antibodies:

Buffer

Number of washes



9)Detection method

10) How many times have you run this staining?

Do you obtain the same results every time?

What steps have you altered to try and optimize the use of this antibody?



Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem.





Please send this questionnaire by e-mail by cutting and pasting the text in your e-mail (no attachments other than images) to technical@abcam.com.

Thanks for your email.

This item is currently back ordered 1-2 weeks from date the order is placed. You should expect to receive this vial of ab64171 in approximately 1 week.

I hope this is helpful. Please contact us again if you have any further questions.

Fantastic! Best of luck in your experiments!

Thank you for your enquiry.

We have a number of different antibodies against specific acetylated residues on histone H4. However an antibody does not exist that will detect every acetylated residue on histone H4.

You may consider just using one or two antibodies against commonly acetylated histone H4 residues. I'm sure this information is available in the literature.

I hope this is helpful. Please contact me again if you have any further questions.

DISCOUNT CODE: 100%ABR-T7A9Q
Expiration date: 12th August 2012

I am very pleased to hear you would like to accept our offer and test ab7311 insheep tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forsheep tissueand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.