AB10158

Anti-Histone H4 抗体 - ChIP Grade

Name: Histone H4抗体- ChIP Grade (ab10158)| Abcam中文官网
Brand: Abcam Limited
SKU: AB10158
Price: 4824 CNY
Availability: InStock
Rating: 4 (21 reviews)

Anti-Histone H4 antibody - ChIP Grade

BOND RX™ Validated
了解详情

(21 Reviews)

(358 Publications)

Anti-Histone H4 antibody - ChIP Grade (ab10158) is a rabbit polyclonal antibody detecting Histone H4 in Western Blot, IP, IHC-P, ChIP. Suitable for Human, Mouse, Rat.

- Over 300 publications
- Trusted since 2004

查看别名

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4

5 Images

Unknown

Western blot - Anti-Histone H4 antibody - ChIP Grade (AB10158)

All lanes:

Western blot - Anti-Histone H4 antibody - ChIP Grade (ab10158) at 1 µg/mL

Lanes 1 and 3:

Histone prep at 20 µg

Lane 2:

HeLa histone lysate at 20 µg

Lane 4:

HeLa Histone lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody - ChIP Grade (AB10158)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody - ChIP Grade (AB10158)

IHC image of Histone H4 staining in human breast carcinoma FFPE section, performed on a Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10158, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

ChIP - Anti-Histone H4 antibody - ChIP Grade (AB10158)

ChIP

Unknown

ChIP - Anti-Histone H4 antibody - ChIP Grade (AB10158)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab10158 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Unknown

Immunoprecipitation - Anti-Histone H4 antibody - ChIP Grade (AB10158)

Histone H4 was immunoprecipitated using 0.5mg NIH/3T3 whole cell lysate, 5μg of Rabbit polyclonal to Histone H4 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70^oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10158.
Secondary : Clean blot (HRP conjugate) at 1/1000 dilution.
Band : 14kDa : Histone H4; non specific - 52 and 85kDa : We are unsure as to the identity of this extra band.

All lanes:

Immunoprecipitation - Anti-Histone H4 antibody - ChIP Grade (ab10158)

false

Project

Western blot - Anti-Histone H4 antibody - ChIP Grade (AB10158)

All lanes:

Western blot - Anti-Histone H4 antibody - ChIP Grade (ab10158) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

ChIP, IP, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

From May 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2 µg for 25 µg chromatin", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Caenorhabditis elegans": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Saccharomyces cerevisiae": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-Histone H4 antibody - ChIP Grade (ab10158) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ChIP in Human, Mouse, Rat samples.

What is the molecular weight of Histone H4?
Anti-Histone H4 - ChIP Grade (ab10158) specifically detects a band for Histone H4 (UniProt: P62805) at a molecular weight of 11kDa.

Trusted by the scientific community
Anti-Histone H4 - ChIP Grade (ab10158) was first used in a scientific publication in 2004 and has been cited over 300 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H4 - ChIP Grade (ab10158) has over 15 independent reviews from customers.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H4C1

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文献 (358)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40966523

2025

Post-translational modification of H2B C-terminal helix regulates nucleosome interactions and chromatin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yani Zhao,Anh Nguyen,Eyla C Arteaga,Aleksandra Skrajna,Krzysztof Krajewski,Dennis Goldfarb,Robert K McGinty

PLoS pathogens 21:e1012501 PubMed40834051

2025

Daxx mediated histone H3.3 deposition on HSV-1 DNA restricts genome decompaction and the progression of immediate-early transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley P E Roberts,Anne Orr,Victor Iliev,Lauren Orr,Steven McFarlane,Zhousiyu Yang,Ilaria Epifano,Colin Loney,Mila Collados Rodriguez,Anna R Cliffe,Kristen L Conn,Chris Boutell

Cell communication and signaling : CCS 23:331 PubMed40640803

2025

NASP implication in the androgen receptor associated with castration resistance in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Feng,Jin Sun,Xuan Kang,Yining Wang,Kai Liu,Wenfei Wang,Chen Wang,Yabin Li,Qian Zhao,Kaile Li,Fang-Lin Sun,Jianfeng Chang

Journal of medicinal chemistry 68:13295-13320 PubMed40526927

2025

Structure-Guided Design of a KMT9 Inhibitor Prodrug with Cellular Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Wang,Nicolas P F Barthes,Sylvia Urban,Viktor I Hazai,Sebastian O Klein,Tabea Pappert,Paul Kümmel,Nicolas Heller,Johannes Bacher,Maximilian Staudt,Jan Ruprecht,Ling Peng,Manuela Sum,Christopher Berlin,Daad Sarraf,Pierre Regenass,Robin Warstat,Johannes Walz,Pankaj Mishra,Lin Zhang,Oliver Einsle,Stefan Günther,Bernhard Breit,Eric Metzger,Manfred Jung,Roland Schüle

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism :271678X251328179 PubMed40370303

2025

Environmental enrichment promotes functional recovery from stroke via enhancing neuroplasticity through the action of -HB.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuang-Yin Qu,Chang-Jing Zhang,Ya-Lan Hou,Hui-Lin Li,Long Lin,Ai Teng,Chang-Run Shi,Wen-Shuo Lu,Xiao-Wei Zhang,Fei Li,Lei Chang,Yu-Hui Lin

Nature communications 16:4030 PubMed40301306

2025

PKA plays a conserved role in regulating gene expression and metabolic adaptation by phosphorylating Rpd3/HDAC1.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Dai,Qi Yu,Rui Ma,Zhu Zheng,Lingling Hong,Yuqing Qi,Fei He,Min Wang,Feng Ge,Xilan Yu,Shanshan Li

Epigenetics 20:2498859 PubMed40296586

2025

Higher sperm H3K4me3 levels are associated with idiopathic recurrent pregnancy loss.

Applications

Unspecified application

Species

Unspecified reactive species

Delna Irani,Deepshikha Arya,Deepti Tandon,Vandana Bansal,Anushree Patil,Dipty Singh

Nature communications 16:3492 PubMed40221437

2025

Mutant CEBPA promotes tolerance to inflammatory stress through deficient AP-1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Cadefau-Fabregat,Gerard Martínez-Cebrián,Lucía Lorenzi,Felix D Weiss,Anne-Katrine Frank,José Manuel Castelló-García,Eric Julià-Vilella,Andrés Gámez-García,Laura Yera,Carini Picardi Morais de Castro,Yi-Fang Wang,Felix Meissner,Alejandro Vaquero,Matthias Merkenschlager,Bo T Porse,Sergi Cuartero

Nature communications 16:2379 PubMed40064919

2025

Competitive antagonism of KAT7 crotonylation against acetylation affects procentriole formation and colorectal tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,Guanqun Mu,Bingquan Qiu,Shuo Wang,Changyu Tao,Yutong Mao,Xinhui Zhao,Jiansong Liu,Keyu Chen,Ziyu Li,Weibin Wang,Ence Yang,Yang Yang

Nucleic acids research 53: PubMed39997219

2025

DNA replication dynamics are associated with genome composition in Plasmodium species.

Applications

Unspecified application

Species

Unspecified reactive species

Francis Isidore Garcia Totañes,Sarah E Chapman,Subash Kumar Rai,Mathew J K Jones,Michael A Boemo,Catherine J Merrick

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H4 抗体 - ChIP Grade

Anti-Histone H4 antibody - ChIP Grade

Western blot - Anti-Histone H4 antibody - ChIP Grade (AB10158)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody - ChIP Grade (AB10158)

ChIP - Anti-Histone H4 antibody - ChIP Grade (AB10158)

Immunoprecipitation - Anti-Histone H4 antibody - ChIP Grade (AB10158)

Western blot - Anti-Histone H4 antibody - ChIP Grade (AB10158)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性