AB272144

Anti-Histone H3 (tri methyl K4，phospho T6)抗体

Name: Histone H3 (tri methyl K4, 磷酸化T6)抗体 (ab272144)| Abcam中文官网
Brand: Abcam Limited
SKU: AB272144
Price: 5789 CNY
Availability: InStock

Anti-Histone H3 (tri methyl K4, phospho T6) antibody

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Rabbit Polyclonal H3 tri methyl K4 + phospho T6 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Caenorhabditis elegans, Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Human H3C15.

查看别名

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

Immunofluoescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (tri methyl K4, phospho T6) with ab272144 at a 1/50 dilution for 1 hour at room temperature in ICC/IF (Green). FITC secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.

ChIP - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

ChIP

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ChIP - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

ChIP of Histone H3 (tri methyl K4, phospho T6). Chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells was used with 2 μg of ab272144 to IP DNA from fixed HeLa cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).

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Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (ab272144) at 1/500 dilution

All lanes:

Caenorhabditis elegans embryo lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Unknown

Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (ab272144) at 1/500 dilution

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparations at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Unknown

Dot Blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

Dot Blot analysis of Histone H3 (tri methyl K4, phospho T6) labeled with ab272144 at 1/1000 dilution.

Lane 1 : Unmodified.

Lane 2 : pT6.

Lane 3 : K4Me1pT6.

Lane 4 : K4Me2pT6.

Lane 5 : K4Me3pT6.

Load : 3, 1, and 0.3 picomoles of peptide.

Block : 5% BLOTTO overnight at 4°C.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human, Caenorhabditis elegans

应用

ChIP, Dot, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human H3C15. The exact immunogen used to generate this antibody is proprietary information.

Q71DI3

特异性

This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg/cells for 6 µg/cells", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

纯化说明

Anti-Histone H3 [Trimethyl Lys4, p Thr6] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

存储溶液

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Do Not Freeze

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 tri methyl K4 + phospho T6

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (tri methyl K4，phospho T6)抗体

Anti-Histone H3 (tri methyl K4, phospho T6) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

ChIP - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

Dot Blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (AB272144)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性