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AB272155

Anti-Histone H3 (tri methyl K27, phospho S28) 抗体

Anti-Histone H3 (tri methyl K27, phospho S28) antibody

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(1 Publication)

Rabbit Polyclonal H3 tri methyl K27 + phospho S28 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Mouse, Caenorhabditis elegans, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C1 tri methyl K27 + phospho S28.

查看别名

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (AB272155)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (AB272155)

Immunofluorescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (tri methyl K27, phospho S28) using ab272155 at a 1/2000 dilution for 1 hour at room temperature (Green). Dylight 488 secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (AB272155)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (AB272155)

Immunofluorescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (tri methyl K27, phospho S28) using ab272155 at a 1/2000 dilution for 1 hour at room temperature. Dylight 488 secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.

ChIP - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (AB272155)
  • ChIP

Unknown

ChIP - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (AB272155)

ChIP analysis of chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using 2μg of ab272155 and 20μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Western blot - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (AB272155)
  • WB

Unknown

Western blot - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (AB272155)

Primary incubation overnight at 4°C.

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (ab272155) at 1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparation

Lane 2:

NIH/3T3 (Mouse embryo fibroblast cell line) histone preparation

Lane 3:

Caenorhabditis elegans embryo lysate

Predicted band size: 15 kDa

false

Dot Blot - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (AB272155)
  • Dot

Unknown

Dot Blot - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (AB272155)

Dot Blot analysis of Histone H3 (tri methyl K27, phospho S28) using ab272155 at 1/1000 dilution.

Lane 1 : pS28/K27 unmodified.

Lane 2 : pS28 N-Term.

Lane 3 : p28 C-term.

Lane 4 : K27Me3.

Lane 5 : pS28/K27Me3.

Load : 1, 10, and 100 picomoles of peptide.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human, Caenorhabditis elegans

应用

WB, Dot, ICC/IF, ChIP

applications

免疫原

Synthetic Peptide within Human H3C1 tri methyl K27 + phospho S28. The exact immunogen used to generate this antibody is proprietary information.

P68431

特异性

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg/cells for 6 µg/cells", "ChIP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
纯化说明
Anti-Histone H3 [Trimethyl Lys27, p Ser28] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
存储溶液
pH: 7.2 Preservative: 0.05% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3C1 tri methyl K27 + phospho S28
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:11059-11070 PubMed33149618

2020

The Pseudogene DUXAP8 Promotes Colorectal Cancer Cell Proliferation, Invasion, and Migration by Inducing Epithelial-Mesenchymal Transition Through Interacting with EZH2 and H3K27me3.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing He,Yi Yu,Wei Huang,Guoliang Feng,Junhe Li
View all publications
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搭配使用产品

Primary Antibodies

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Proteins & Peptides

AB1782

Human Histone H3 (tri methyl K27) peptide

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  • 5
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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

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