AB272156

Anti-Histone H3 (symmetric di methyl R2, di methyl K4) 抗体

Name: Histone H3 (symmetric di methyl R2, di methyl K4)抗体 (ab272156)| Abcam中文官网
Brand: Abcam Limited
SKU: AB272156
Price: 5847 CNY
Availability: InStock

Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody

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(1 Publication)

Rabbit Polyclonal H3 symmetric di methyl R2 + di methyl K4 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Mouse, Caenorhabditis elegans, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C15.

查看别名

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3C1, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

Immunofluorescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (symmetric di methyl R2, di methyl K4) using ab272156 at 1/100 dilution for 1 hour at room temperature (Green). Dylight 488 secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.

ChIP - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

ChIP

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ChIP - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

ChIP analysis of chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using 2μg of ab272156 and 20μl of magnetic IgG beads per immunoprecipitation. A "no antibody" (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one

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Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

Primary incubation overnight at 4°C.

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

All lanes:

Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (ab272156) at 1/500 dilution

All lanes:

NIH/3T3 (Mouse embryo fibroblast cell line) histone preparation at 30 µg

Predicted band size: 15 kDa

false

Unknown

Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

Primary incubation overnight at 4°C.

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

All lanes:

Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (ab272156) at 1/500 dilution

All lanes:

Caenorhabditis elegans embryo lysate at 30 µg

Predicted band size: 15 kDa

false

Unknown

Dot Blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

Dot Blot analysis of Histone H3 (symmetric di methyl R2, di methyl K4) using ab272156 at 1/1000 dilution.

Lane 1 : R2Me2.

Lane 2 : R2Me2/K4Me2.

Lane 3 : K4Me2.

Load : 0.1, 1, 0.5, 5, and 10 picomoles of peptide.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human, Caenorhabditis elegans

应用

Dot, ICC/IF, WB, ChIP

applications

免疫原

Synthetic Peptide within Human H3C15. The exact immunogen used to generate this antibody is proprietary information.

Q71DI3

特异性

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg/cells for 6 µg/cells", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

纯化说明

Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

存储溶液

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 symmetric di methyl R2 + di methyl K4

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:11059-11070 PubMed33149618

2020

The Pseudogene DUXAP8 Promotes Colorectal Cancer Cell Proliferation, Invasion, and Migration by Inducing Epithelial-Mesenchymal Transition Through Interacting with EZH2 and H3K27me3.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing He,Yi Yu,Wei Huang,Guoliang Feng,Junhe Li

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (symmetric di methyl R2, di methyl K4) 抗体

Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

ChIP - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

Dot Blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性