Anti-Histone H3 (phospho T6，tri methyl K9)抗体(ab272147)
Key features and details
- Rabbit polyclonal to Histone H3 (phospho T6, tri methyl K9)
- Suitable for: ChIP, WB, Dot blot, ICC/IF
- Reacts with: Human, Caenorhabditis elegans
- Isotype: IgG
概述
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产品名称
Anti-Histone H3 (phospho T6，tri methyl K9)抗体
参阅全部 Histone H3 一抗 -
描述
兔多克隆抗体to Histone H3 (phospho T6，tri methyl K9) -
宿主
Rabbit -
特异性
A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
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经测试应用
适用于: ChIP, WB, Dot blot, ICC/IFmore details -
种属反应性
与反应: Human, Caenorhabditis elegans -
免疫原
Synthetic peptide corresponding to Human Histone H3 (phospho T6, tri methyl K9).
Database link: Q71DI3 -
阳性对照
- WB: Caenorhabditis elegans embryo lysate; HeLa histone preparation. ICC/IF: HeLa cells. ChIP: Chromatin prepared from HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Anti-Histone H3 [Trimethyl Lys9, p Thr6] was affinity purified from monospecific antiserum by immunoaffinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272147于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ChIP |
Use 2-5µg for 106 cells.
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| WB |
1/500. Predicted molecular weight: 15 kDa.
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| Dot blot |
1/1000.
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| ICC/IF |
1/50 - 1/200.
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| 说明 |
|---|
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ChIP
Use 2-5µg for 106 cells. |
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WB
1/500. Predicted molecular weight: 15 kDa. |
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Dot blot
1/1000. |
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ICC/IF
1/50 - 1/200. |
靶标
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功能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
序列相似性
Belongs to the histone H3 family. -
发展阶段
Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. -
翻译后修饰
Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 8350 Human
- Entrez Gene: 8351 Human
- Entrez Gene: 8352 Human
- Entrez Gene: 8353 Human
- Entrez Gene: 8354 Human
- Entrez Gene: 8355 Human
- Entrez Gene: 8356 Human
- Entrez Gene: 8357 Human
see all -
别名
- H3 histone family member E pseudogene antibody
- H3 histone family, member A antibody
- H3/A antibody
see all
图片
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Western blot - Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147)Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147) at 1/500 dilution + Caenorhabditis elegans embryo lysate at 30 µg
Predicted band size: 15 kDaPrimary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147)Immunofluoescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (phospho T6, tri methyl K9) using ab272147 at a 1/50 dilution for 1 hour at room temperature (Green). FITC secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue).
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Western blot - Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147)Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147) at 1/500 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparation at 30 µg
Predicted band size: 15 kDaPrimary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
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ChIP - Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147)ChIP analysis of chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using 2µg of ab272147 and 20µl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
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Dot Blot - Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147)Dot Blot analysis of Histone H3 (phospho T6, tri methyl K9) using ab272147 at 1/1000 dilution.
Lane 1: T6.
Lane 2: pT6.
Lane 3: pT6 K9KMe1.
Lane 4: pT6 K9KMe2.
Lane 5: pT6 K9KMe3.
Lane 6: K9.
Lane 7: K9KMe1.
Lane 8: K9KMe2.
Lane 9: K9KMe3.
Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272147 尚未被引用在任何文献中。