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AB8895

Anti-Histone H3 (mono methyl K4) 抗体 - ChIP Grade

Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade

4

(58 Reviews)

|

(1121 Publications)

Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ChIP. Suitable for Cow, Human, Mouse, Rat.

- Over 1120 publications
- Trusted since 2002

查看别名

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me, H3K4me1, H3K4

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

IHC image of ab8895 staining Histone H3 (mono methyl K4) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8895, 0.5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

ab8895 staining Histone H3 (mono methyl K4) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab8895 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

ChIP - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)
  • ChIP

AbReview65555****

ChIP - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

ChIP analysis using ab8895 binding Histone H3 (mono methyl K4) in Metastatic clear cell renal carcinoma cells (M1A). Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated at 1/100 dilution with primary antibody for 12 hours at 4°C.
Positive control : Metastatic clear cell renal carcinoma cells (M1A).
Negative Control : Parental clear cell renal carcinoma cells (786-O).

This image is courtesy of an Abreview submitted by Paulo Rodrigues

ChIP - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)
  • ChIP

Unknown

ChIP - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

Chromatin was prepared from U-2 OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab8895 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH and ALDOA (active) and MYO-D (inactive) promoters and over the y-Actin gene (active). Schematic diagram of the y-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)
  • WB

Lab

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

Blocking buffer : 2% BSA

Gel type : MES

All lanes:

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) at 1 µg/mL

Lane 1:

Calf Thymus Histone at 0.5 µg

Lane 2:

HeLa Nuclear – Triton Prep at 10 µg

Lane 3:

NIH3T3 Nuclear – Triton Prep at 10 µg

Lane 4:

PC12 Nuclear at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 30s

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)
  • WB

Unknown

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

ab8895 is specific for mono-methylated Lysine 4 of histone H3 and does not recognize di- or tri-methyl Lysine 4 nor methylation at Lysine 9. This is shown in lane 2 where the activity of the antibody is specifically blocked by the addition of the immunizing peptide (ab1340).

All lanes:

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) at 1/500 dilution

All lanes:

Calf thymus histone lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 18 kDa

false

Exposure time: 2min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Cow, Human, Mouse, Rat

应用

IHC-P, WB, ChIP, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Specific for mono-methylated Lysine 4 of histone H3. Does not recognise di- or tri-methyl Lysine 4 nor methylation at Lysine 9.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Works better if cells fixed in methanol.</p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2 µg for 25 µg chromatin", "ChIP-species-notes": "<p>We recommend Myo-D ChIP primer pair <a href='/products/reagents/myo-d-positive-control-chip-primer-pair-ab269261'>ab269261</a> as positive control.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1 - 1/5", "WB-species-notes": "<p></p>" }, "Candida albicans": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Drosophila melanogaster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mammals": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Plants": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Plasmodium falciparum": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Saccharomyces cerevisiae": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Tetrahymena": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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您是否考虑以下替代产品？

AB176877

Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade

4
7 Reviews
查看产品
为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 mono methyl K4
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文献 (1121)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:108624 PubMed38174321

2024

Crosstalk between chromatin and Shavenbaby defines transcriptional output along the Drosophila intestinal stem cell lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Mancheno-Ferris,Clément Immarigeon,Alexia Rivero,David Depierre,Naomi Schickele,Olivier Fosseprez,Nicolas Chanard,Gabriel Aughey,Priscilla Lhoumaud,Julien Anglade,Tony Southall,Serge Plaza,François Payre,Olivier Cuvier,Cédric Polesello

Communications biology 7:10 PubMed38172644

2024

STAT3 and SOX-5 induce BRG1-mediated chromatin remodeling of RORCE2 in Th17 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xian Wang,Chao Han,Di Yang,Jian Zhou,Hui Dong,Zhiyuan Wei,Shuai Xu,Chen Xu,Yiwei Zhang,Yi Sun,Bing Ni,Sheng Guo,Jingbo Zhang,Tingting Zhao,Xiangmei Chen,Jie Luo,Yuzhang Wu,Yi Tian

Scientific reports 13:22565 PubMed38114530

2023

Sequential deregulation of histone marks, chromatin accessibility and gene expression in response to PROTAC-induced degradation of ASH2L.

Applications

Unspecified application

Species

Unspecified reactive species

Mirna Barsoum,Roksaneh Sayadi-Boroujeni,Alexander T Stenzel,Philip Bussmann,Juliane Lüscher-Firzlaff,Bernhard Lüscher

Nature communications 14:8372 PubMed38102116

2023

BRD9 determines the cell fate of hematopoietic stem cells by regulating chromatin state.

Applications

Unspecified application

Species

Unspecified reactive species

Muran Xiao,Shinji Kondo,Masaki Nomura,Shinichiro Kato,Koutarou Nishimura,Weijia Zang,Yifan Zhang,Tomohiro Akashi,Aaron Viny,Tsukasa Shigehiro,Tomokatsu Ikawa,Hiromi Yamazaki,Miki Fukumoto,Atsushi Tanaka,Yasutaka Hayashi,Yui Koike,Yumi Aoyama,Hiromi Ito,Hiroyoshi Nishikawa,Toshio Kitamura,Akinori Kanai,Akihiko Yokoyama,Tohru Fujiwara,Susumu Goyama,Hideki Noguchi,Stanley C Lee,Atsushi Toyoda,Kunihiko Hinohara,Omar Abdel-Wahab,Daichi Inoue

Nature genetics 56:100-111 PubMed38049663

2023

The BAF chromatin remodeler synergizes with RNA polymerase II and transcription factors to evict nucleosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Sandipan Brahma,Steven Henikoff

Cell death & disease 14:777 PubMed38012140

2023

ΔNp63α facilitates proliferation and migration, and modulates the chromatin landscape in intrahepatic cholangiocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anghui Peng,Xiaowen Lin,Quanli Yang,Yihao Sun,Ruiyan Chen,Bing Liu,Xinyang Yu

Nature communications 14:7430 PubMed37973845

2023

Repression of LSD1 potentiates homologous recombination-proficient ovarian cancer to PARP inhibitors through down-regulation of BRCA1/2 and RAD51.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Tao,Yue Zhou,Xiangyu Pan,Yuan Luo,Jiahao Qiu,Xia Zhou,Zhiqian Chen,Yan Li,Lian Xu,Yang Zhou,Zeping Zuo,Chunqi Liu,Liang Wang,Xiaocong Liu,Xinyu Tian,Na Su,Zhengnan Yang,Yu Zhang,Kun Gou,Na Sang,Huan Liu,Jiao Zou,Yuzhou Xiao,Xi Zhong,Jing Xu,Xinyu Yang,Kai Xiao,Yanyang Liu,Shengyong Yang,Yong Peng,Junhong Han,Xiaobo Cen,Yinglan Zhao

Journal of translational medicine 21:716 PubMed37828515

2023

Dissecting transcription of the 8q24-MYC locus in prostate cancer recognizes the equilibration between androgen receptor direct and indirect dual-functions.

Applications

Unspecified application

Species

Unspecified reactive species

Ju Guo,Zhao Wei,Tianwei Jia,Liyang Wang,Nuosu Nama,Jiaqian Liang,Xinghua Liao,Xiaming Liu,Yanfei Gao,Xiaoqiang Liu,Keshan Wang,Bin Fu,Shaoyong Shawn Chen

Communications biology 6:1030 PubMed37821650

2023

MUC1-C intersects chronic inflammation with epigenetic reprogramming by regulating the set1a compass complex in cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Atrayee Bhattacharya,Atsushi Fushimi,Keyi Wang,Nami Yamashita,Yoshihiro Morimoto,Satoshi Ishikawa,Tatsuaki Daimon,Tao Liu,Song Liu,Mark D Long,Donald Kufe

Pharmaceutics 15: PubMed37896200

2023

Epidrugs as Promising Tools to Eliminate Artemisinin-Resistant and Quiescent Parasites.

Applications

Unspecified application

Species

Unspecified reactive species

Thibaud Reyser,Lucie Paloque,Michel Nguyen,Jean-Michel Augereau,Matthew John Fuchter,Marie Lopez,Paola B Arimondo,Storm Hassell-Hart,John Spencer,Luisa Di Stefano,Françoise Benoit-Vical
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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