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AB272160

Anti-Histone H3 (mono methyl K37)抗体

Anti-Histone H3 (mono methyl K37) antibody

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Rabbit Polyclonal H3 mono methyl K37 antibody. Suitable for Dot, WB, IF and reacts with Synthetic peptide, Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human H3C1 mono methyl K37.

查看别名

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K37me, H3K37me1, H3K37

4 Images
Immunofluorescence - Anti-Histone H3 (mono methyl K37) antibody (AB272160)
  • IF

Supplier Data

Immunofluorescence - Anti-Histone H3 (mono methyl K37) antibody (AB272160)

Immunofluorescence analysis of Anti-Histone H3K37me. ab272160 was tested at 1 : 200 in HeLa cells with FITC (green). Cells and nuclei were counterstained with DAPI (blue) and DyLight 550 (red). (40X)

Western blot - Anti-Histone H3 (mono methyl K37) antibody (AB272160)
  • WB

Unknown

Western blot - Anti-Histone H3 (mono methyl K37) antibody (AB272160)

Block : 5% BLOTTO overnight at 4°C.

All lanes:

Western blot - Anti-Histone H3 (mono methyl K37) antibody (ab272160) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

NIH/3T3 cell lysate at 30 µg

Lane 3:

Elegans embryo lysate at 30 µg

Secondary

All lanes:

RDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT at 1/10000 dilution

Predicted band size: 15 kDa

false

Dot Blot - Anti-Histone H3 (mono methyl K37) antibody (AB272160)
  • Dot

Unknown

Dot Blot - Anti-Histone H3 (mono methyl K37) antibody (AB272160)

Dot Blot analysis of ab272160.

Lane 1 : K37me1.

Lane 2 : K36me1.

Lane 3 : K36me2.

Lane 4 : K36me3.

Lane 5 : K36ac.

Load : 1, 10, and 100 picomoles of peptide. Primary antibody : ab272160 at 1 : 1000 for 45 minutes at 4°C. Secondary antibody : Dylight™488 rabbit secondary antibody at 1 : 10,000 for 45 minutes at RT. Block : 5% BLOTTO overnight at 4°C.

Dot Blot - Anti-Histone H3 (mono methyl K37) antibody (AB272160)
  • Dot

Unknown

Dot Blot - Anti-Histone H3 (mono methyl K37) antibody (AB272160)

Dot Blot analysis of ab272160.

Lane 1 : K37.

Lane 2 : K37Ac.

Lane 3 : K37Me1.

Lane 4 : K37Me2.

Lane 5 : K37Me3.

Load : 1, 10, and 100 picomoles of peptide. Primary antibody : ab272160 at 1 : 1000 for 45 minutes at 4°C. Secondary antibody : Dylight™488 rabbit secondary antibody at 1 : 10,000 for 45 minutes at room temperature. Block : 5% BLOTTO overnight at 4°C.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

WB, Dot, IF

applications

免疫原

Synthetic Peptide within Human H3C1 mono methyl K37. The exact immunogen used to generate this antibody is proprietary information.

P68431

特异性

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IF" : {"fullname" : "Immunofluorescence", "shortname":"IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IF-species-checked": "testedAndGuaranteed", "IF-species-dilution-info": "1/200", "IF-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IF-species-checked": "guaranteed", "IF-species-dilution-info": "", "IF-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IF-species-checked": "notRecommended", "IF-species-dilution-info": "", "IF-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Anti-Histone H3 [Monomethyl Lys37] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
存储溶液
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3C1 mono methyl K37
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搭配使用产品

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Primary Antibodies

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Anti-Histone H3 (tri methyl K27, phospho S28) antibody

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  • 0
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Primary Antibodies

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Primary Antibodies

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