Anti-Histone H3 (di methyl K4)抗体- ChIP Grade
Anti-Histone H3 (di methyl K4) antibody - ChIP Grade
5
(25 Reviews)
|
(320 Publications)
Rabbit Polyclonal H3 di methyl K4 antibody. Suitable for ICC/IF, ChIP, WB, PepArr and reacts with Human, Mouse, Rat, Cow, Synthetic peptide - Human samples. Cited in 320 publications.
查看别名
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me2, H3K4me, H3K4
- FuncS
Unknown
Functional Studies - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
MCF7 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of tranylcypromine hydrochloride (ab120606). Increased expression of Histone 3 K4 di-methyl (ab7766) in MCF7 cells correlates with an increase in tranylcypromine hydrochloride concentration, as described in literature.
Nuclear extracts were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab7766 at 1 μg /ml and ab1791 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody (ab97051) at 1/10000 dilution and visualized using ECL development solution.
- ICC/IF
AbReview6832****
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
ab7766 at 1/200 staining human U2OS (osteosarcoma) cells by ICC/IF. The cells were paraformaldehyde fixed and then stained with the antibody for 1 hour. A Cy2 ® conjugated donkey anti-rabbit antibody was used as the secondary (green). The image shows uniformal staining of the whole nucleus, with several specles found. The insert shows H3-di methyl K4 of tw2 cells only. DAPI nuclear staining is shown in blue.
This image is courtesy of an Abreview submitted by Dr Alexander Rapp
- ICC/IF
Collaborator
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
Dimethylated lysine 4 (green) is found in several hundred small nuclear foci that do not colocalize with condensed regions of chromatin (DAPI stained, red). The perinuclear and perinucleolar heterochromatin do not stain with this antibody.
This image is courtesy of Kirk McManus in the lab of Michael Hendzel, Univeristy of Alberta
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
ab7766 staining Histone H3 (di methyl K4) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7766 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
- ChIP
Unknown
ChIP - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab7766 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
- WB
Unknown
Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
All lanes:
Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (ab7766) at 1 µg/mL
All lanes:
Calf Thymus Histone Preparation Nuclear Lysate (<a href='/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa,17 kDa
true
Exposure time: 4min
- WB
Unknown
Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
Blocking buffer : 2% BSA
Gel type : MES
Exposure Time : 1 minute
All lanes:
Western blot - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (ab7766) at 1 µg/mL
Lane 1:
HeLa nuclear lysate (triton enriched) at 20 µg
Lane 2:
NIH 3T3 nuclear lysate (triton enriched) at 20 µg
Lane 3:
PC12 nuclear lysate (triton enriched at 20 µg
Secondary
All lanes:
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa
false
- PepArr
Unknown
Peptide Array - Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (AB7766)
All batches of ab7766 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - di methyl K4 peptide (ab7768), indicating that this antibody specifically recognises the Histone H3 - di methyl K4 modification.
- ab1340 - Histone H3 - mono methyl K4
- ab1342 - Histone H3 - tri methyl K4
- ab1771 - Histone H3 - mono methyl K9
- ab1772 - Histone H3 - di methyl K9
- ab1773 - Histone H3 - tri methyl K9
- ab1780 - Histone H3 - mono methyl K27
- ab1781 - Histone H3 - di methyl K27
- ab1782 - Histone H3 - tri methyl K27
- ab7228 - Histone H3 - unmodified
- ab7768 - Histone H3 - di methyl K4
反应性数据
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Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade
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产品详情
For detection of Histone H3 specifically methylated at position Lys 4. This antibody was used in a screen by Dover et al, (2002) to isolate yeast mutants that are unable to methylate Lysine 4. In immunofluorescence, this antibody detects foci in the nucleus that are non-colocalising with condensed chromatin. The perinuclear and perinucleolar heterochromatin are not stained with this antibody.
Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
文献 (320)
Recent publications for all applications. Explore the full list and refine your search
Communications biology 7:10 PubMed38172644
2024
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Journal of translational medicine 21:716 PubMed37828515
2023
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Molecular nutrition & food research 67:e2300040 PubMed37672803
2023
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Cellular and molecular life sciences : CMLS 80:264 PubMed37615725
2023
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Nature communications 14:3502 PubMed37311740
2023
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Nature communications 14:910 PubMed36801853
2023
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Nature communications 14:603 PubMed36746939
2023
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Nucleic acids research 50:12809-12828 PubMed36537238
2022
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Scientific reports 12:21506 PubMed36513698
2022
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Journal of integrative plant biology 64:2438-2454 PubMed36354145
2022
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