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AB9049

Anti-Histone H3 (di methyl K36)抗体- ChIP Grade

Anti-Histone H3 (di methyl K36) antibody - ChIP Grade

5

(24 Reviews)

|

(141 Publications)

Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (ab9049) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ChIP. Suitable for Cow, Drosophila melanogaster, Human.

- Over 140 publications
- Trusted since 2003

查看别名

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K36me2, H3K36me, H3K36

4 Images
ChIP - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)
  • ChIP

Unknown

ChIP - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 2 μg of ab9049(blue), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH and RPL30 (active) promoters and over the g-Actin gene (active). Schematic diagram of the g-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)
  • WB

AbReview25030****

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

All lanes:

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (ab9049) at 1/1400 dilution

All lanes:

Whole cell lysate prepared from Drosophila BG3 tissue cells at 50000 Cells

Secondary

All lanes:

Donkey anti-rabbit polyclonal conjugated to HRP at 1/20000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa,42 kDa

true

Exposure time: 30s

Image courtesy of an anonymous Abreview.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

IHC image of ab9049 staining Histone H3 (di methyl K36) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9049, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)
  • WB

Project1016****

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

All lanes:

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (ab9049) at 1 µg/mL

Lanes 1, 4, 5, 6, 7, 8, 9, 10 and 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H3 peptide (ab41430)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K36) peptide (<a href='/products/unavailable/human-histone-h3-mono-methyl-k36-peptide-ab1783'>ab1783</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Cow, Human, Drosophila melanogaster

应用

WB, IHC-P, ChIP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody is specific for histone H3 di-methylated at K36. It does not react with mono or tri methyl K36.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg for 25 µg chromatin", "ChIP-species-notes": "<p>Use RPL30 ChIP primer pair <a href='/products/reagents/rpl30-positive-control-chip-primer-pair-ab269262'>ab269262</a> as positive control.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Arabidopsis thaliana": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Caenorhabditis elegans": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Dictyostelium discoideum": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mammals": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Saccharomyces cerevisiae": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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您是否考虑以下替代产品？

AB176921

Anti-Histone H3 (di methyl K36) antibody [EPR16994(2)] - ChIP Grade

3
7 Reviews
查看产品
为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 di methyl K36
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文献 (141)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 7: PubMed38011998

2023

2-Hydroxyglutarate modulates histone methylation at specific loci and alters gene expression via Rph1 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Marios Gavriil,Marco Proietto,Nicole Paczia,Aurelien Ginolhac,Rashi Halder,Elena Valceschini,Thomas Sauter,Carole L Linster,Lasse Sinkkonen

Life science alliance 6: PubMed37684044

2023

Chromatin in 3D distinguishes dMes-4/NSD and Hypb/dSet2 in protecting genes from H3K27me3 silencing.

Applications

Unspecified application

Species

Unspecified reactive species

David Depierre,Charlène Perrois,Naomi Schickele,Priscillia Lhoumaud,Mahdia Abdi-Galab,Olivier Fosseprez,Alexandre Heurteau,Raphaël Margueron,Olivier Cuvier

Molecular nutrition & food research 67:e2300040 PubMed37672803

2023

Cross-Talk between miRNAs from the Dlk1-Dio3 Locus and Histone Methylation to Protect Male Cerebellum from Methyl Donor Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy Willekens,Pauline Mosca,Nathan Burt-Oberecken,Edgar Laugeais,Tony Kaoma,François Bernardin,Laurent Vallar,Pauline Dimofski,Mathilde Renaud,Laetitia Lambert,Bruno Leheup,Jean-Louis Guéant,Brigitte Leininger-Muller,Natacha Dreumont

Biotechnology for biofuels and bioproducts 16:129 PubMed37592325

2023

Remodeling of the 3D chromatin architecture in the marine microalga Nannochloropsis oceanica during lipid accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Tongtong Yan,Kexin Wang,Kexin Feng,Xiangchen Gao,Yinghong Jin,Hongping Wu,Wenfei Zhang,Li Wei

Journal of cardiovascular development and disease 10: PubMed37504562

2023

H3K36 Di-Methylation Marks, Mediated by Ash1 in Complex with Caf1-55 and MRG15, Are Required during Heart Development.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Yi Zhu,Chen Liu,Xiaohu Huang,Joyce van de Leemput,Hangnoh Lee,Zhe Han

Nature communications 14:4081 PubMed37429872

2023

Histone H1 facilitates restoration of H3K27me3 during DNA replication by chromatin compaction.

Applications

Unspecified application

Species

Unspecified reactive species

Cuifang Liu,Juan Yu,Aoqun Song,Min Wang,Jiansen Hu,Ping Chen,Jicheng Zhao,Guohong Li

Nature communications 14:3892 PubMed37393406

2023

ASH1L-MRG15 methyltransferase deposits H3K4me3 and FACT for damage verification in nucleotide excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Corina Maritz,Reihaneh Khaleghi,Michelle N Yancoskie,Sarah Diethelm,Sonja Brülisauer,Natalia Santos Ferreira,Yang Jiang,Shana J Sturla,Hanspeter Naegeli

PLoS biology 21:e3002161 PubMed37379335

2023

MLL methyltransferases regulate H3K4 methylation to ensure CENP-A assembly at human centromeres.

Applications

Unspecified application

Species

Unspecified reactive species

Kausika Kumar Malik,Sreerama Chaitanya Sridhara,Kaisar Ahmad Lone,Payal Deepakbhai Katariya,Deepshika Pulimamidi,Shweta Tyagi

Experimental & molecular medicine 55:952-964 PubMed37121971

2023

Epigenetic regulation of SMAD3 by histone methyltransferase SMYD2 promotes lung cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Kwangho Kim,Tae Young Ryu,Eunsun Jung,Tae-Su Han,Jinkwon Lee,Seon-Kyu Kim,Yu Na Roh,Moo-Seung Lee,Cho-Rok Jung,Jung Hwa Lim,Ryuji Hamamoto,Hye Won Lee,Keun Hur,Mi-Young Son,Dae-Soo Kim,Hyun-Soo Cho

Chemical biology & drug design 102:500-513 PubMed37072259

2023

Identification of novel class inhibitors of NSD3 methyltransferase showing a unique, bivalent binding mode in the SET domain.

Applications

Unspecified application

Species

Unspecified reactive species

Sumin Kim,Injeoung Hwang,Suhn Hyung Kim,Hwan Won Chung,Mi-Jung Ji,Sojeong Moon,Hyun-Mee Park,Gu Kong,Wooyoung Hur
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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