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Epigenetics and Nuclear Signaling Histones H3 Methylated

Anti-Histone H3 (di methyl K27,tri methyl K27)抗体[mAbcam 6147] (ab6147)

  • Datasheet
  • SDS
Reviews (9)Q&A (1)References (29)

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Western blot - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
  • Western blot - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
  • ELISA - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
  • Flow Cytometry - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)

Key features and details

  • Mouse monoclonal [mAbcam 6147] to Histone H3 (di methyl K27, tri methyl K27)
  • Suitable for: ICC/IF, ELISA, WB, Flow Cyt
  • Reacts with: Mouse, Cow, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Histone H3 (di methyl K27,tri methyl K27)抗体[mAbcam 6147]
    参阅全部 Histone H3 一抗
  • 描述

    小鼠单克隆抗体[mAbcam 6147] to Histone H3 (di methyl K27,tri methyl K27)
  • 宿主

    Mouse
  • 特异性

    By Western blot, this antibody is blocked strongly by di and tri methyl K27 peptides and does not detect a band in Eed KO mouse ES cell lysates (which lack both di and tri methyl K27). All batches of ab6147 have >40% cross reactivity with both H3K27me2 and H3K27me3 as shown by ELISA. The sequence which it reacts with is found in all Mammals and a wide range of other species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the modification is present. Reactivity is not certain in S. pombe and S. cerevisiae as the equivalent protein sequence differs slightly from species listed above.
  • 经测试应用

    适用于: ICC/IF, ELISA, WB, Flow Cytmore details
  • 种属反应性

    与反应: Mouse, Cow, Human
    预测可用于: Rat, Chicken, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster不与反应: Carp
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab1782)

  • 阳性对照

    • Human Lung
  • 常规说明

    Hybridomas were prepared and the resulting clones were positively screened by ELISA against the immunising peptide. Clones were negatively screened against both the corresponding unmodified peptide and also against a peptide corresponding to tri methylated K9 of Histone H3.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    mAbcam 6147
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Immunizing Peptide (Blocking)

    • Human Histone H3 (tri methyl K27) peptide (ab1782)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6147于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (4)
Use a concentration of 1 - 5 µg/ml.
ELISA
Use a concentration of 0.025 - 1 µg/ml.
WB (3)
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human Histone H3 (tri methyl K27) peptide (ab1782).
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

说明
ICC/IF
Use a concentration of 1 - 5 µg/ml.
ELISA
Use a concentration of 0.025 - 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human Histone H3 (tri methyl K27) peptide (ab1782).
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

靶标

  • 功能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 序列相似性

    Belongs to the histone H3 family.
  • 发展阶段

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • 翻译后修饰

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • 细胞定位

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Entrez Gene: 100364501 Rat
    • Entrez Gene: 100365669 Rat
    • Entrez Gene: 291159 Rat
    • Entrez Gene: 679994 Rat
    • Entrez Gene: 680511 Rat
    • Entrez Gene: 682330 Rat
    • Omim: 602810 Human
    • SwissProt: P84229 Chicken
    • SwissProt: P68432 Cow
    • SwissProt: P68431 Human
    • SwissProt: P68433 Mouse
    • SwissProt: Q6LED0 Rat
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 397328 Mouse
    • Unigene: 138090 Rat
    see all
  • 别名

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

图片

  • Western blot - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
    Western blot - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
    All lanes : Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147) at 1 µg/ml

    Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate
    Lane 2 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (unmodified ) peptide (ab2623) at 1 µg
    Lane 3 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (mono methyl K4) peptide (ab1340) at 1 µg
    Lane 4 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (di methyl K4) peptide (ab7768) at 1 µg
    Lane 5 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (tri methyl K4) peptide (ab1342) at 1 µg
    Lane 6 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (mono methyl K9) peptide (ab1771) at 1 µg
    Lane 7 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (di methyl K9) peptide (ab1772) at 1 µg
    Lane 8 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (tri methyl K9) peptide (ab1773) at 1 µg
    Lane 9 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (mono methyl K27) peptide (ab1780) at 1 µg
    Lane 10 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (di methyl K27) peptide (ab1781) at 1 µg
    Lane 11 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (tri methyl K27) peptide (ab1782) at 1 µg

    Lysates/proteins at 0.5 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6147 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
    ICC/IF image of ab6147 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab6147, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Western blot - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
    Western blot - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
    All lanes : Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : EED-/- mouse ES Whole Cell Lysate
    Lane 4 : WT mouse ES Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6147 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • ELISA - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
    ELISA - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)

    All batches of ab6147 are tested in ELISA against peptides to different Histone H3 modifications. Results show strong binding to Histone H3 - tri methyl K27 immunising peptide (ab1782) and Histone H3 - di methyl K27, indicating that this antibody specifically recognises both the Histone H3 - tri methyl K27 and di methyl K27 modifications. Binding is detected against the Histone H3 - di methyl K27 modification (>40%) (ab1781).

    ab2623 - Histone H3 - unmodified

    ab1340 - Histone H3 - mono methyl K4

    ab7768 - Histone H3 - di methyl K4

    ab1342 - Histone H3 - tri methyl K4

    ab1771 - Histone H3 - mono methyl K9

    ab1772 - Histone H3 - di methyl K9

    ab1773 - Histone H3 - tri methyl K9

    ab1780 - Histone H3 - mono methyl K27

    ab1781 - Histone H3 - di methyl K27

    ab1782 - Histone H3 - tri methyl K27

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)Image courtesy of Maki Asami, University of Bath

    ICC/IF image of ab6147 stained mouse 2 cell embryo. Cells were fixed with formaldehyde,  permeabilized with 0.5% Triton, and incubated with ab6147 at 1/50 for 2 hours at 37°C. Blocking was performed in 5% FCS serum for 30 minutes at 37°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG used at a 1/250 dilution. PI was used to stain the DNA (Red).

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)This image is courtesy of an Abreview submitted by Carlos Carvalho
    Immunofluorescence analysis of C. elegans germline cells and gonads, staining Histone H3 (tri methyl K27) with ab6147.

    Cells were fixed with methanol, frozen and cracked in liquid nitrogen and blocked with 0.5% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in diluent) for 14 hours at 4°C. An FITC-conjugated donkey anti-mouse polyclonal IgG (1/300) was used as the secondary antibody.

    See Abreview

  • Flow Cytometry - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
    Flow Cytometry - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)

    Overlay histogram showing HeLa cells stained with ab6147 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6147, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

实验方案

  • Mouse on Mouse staining protocol

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (29)

发表研究结果有使用 ab6147?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6147 被引用在 29 文献中.

  • Salmina K  et al. "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres. Int J Mol Sci 21:N/A (2020). PubMed: 32316332
  • Cao S  et al. The florigen interactor BdES43 represses flowering in the model temperate grass Brachypodium distachyon. Plant J 102:262-275 (2020). PubMed: 31782581
  • Shi Y  et al. C10ORF12 modulates PRC2 histone methyltransferase activity and H3K27me3 levels. Acta Pharmacol Sin 40:1457-1465 (2019). PubMed: 31186533
  • Bi X  et al. RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control. Mol Cell 75:102-116.e9 (2019). PubMed: 31128943
  • Lv L  et al. Long Non-coding RNA LINC00114 Facilitates Colorectal Cancer Development Through EZH2/DNMT1-Induced miR-133b Suppression. Front Oncol 9:1383 (2019). PubMed: 31921641
View all Publications for this product

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1-10 of 10 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid
Permeabilization
Yes - Tween-20
Specification
Human Lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 23 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cell)
Permeabilization
Yes - tween-20
Specification
HeLa cell
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 23 2017

Western blot abreview for Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (K562, MCF7, HCT116, 293T cell)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Loading amount
18 µg
Specification
K562, MCF7, HCT116, 293T cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 22 2017

Western blot abreview for Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (stem cell and progenitor cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
stem cell and progenitor cell
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 19 2016

Western blot abreview for Anti-Histone H3 (di+tri methyl K27) antibody [mAbcam 6147]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary cortical neurons)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
10 µg
Specification
Primary cortical neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 16 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (tri methyl K27) [mAbcam 6147] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample
Human Cell (U2OS)
Specification
U2OS
Permeabilization
Yes - NP40
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 04 2014

Immunocytochemistry/ Immunofluorescence

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Sample
Mouse Cell (2 cell embryo)
Specification
2 cell embryo
Permeabilization
Yes - 0.5% Triton
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Maki Asami

Verified customer

提交于 May 15 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Caenorhabditis elegans Cell (germ cell and embryo)
Permeabilization
Yes - freeze and crack in liquid nitrogen
Specification
germ cell and embryo
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Carlos Carvalho

Verified customer

提交于 Jan 18 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (methylated) antibody [mAbcam 6147]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Methanol
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
Yes - 0.5% Triton X100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Chantal Desmaze

Verified customer

提交于 Dec 07 2011

Question

This is a clinical diagnostics projects, and all antibodies we will use for that need to be CE certified for diagnostic purposes. Since I did not find any information on this on your website, I wanted to ask you if you have any CE certified antibodies directed against these (human) proteins:

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Abcam community

Verified customer

Asked on Feb 02 2012

Answer

All of our products are for research purposes only and are not certified for clinical research applications.

Read More

Abcam Scientific Support

回复于 Feb 02 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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