AB194690

Anti-Histone H3 (di methyl K27)抗体- ChIP Grade

Name: Histone H3 (di methyl K27)抗体- ChIP Grade (ab194690)| Abcam中文官网
Brand: Abcam Limited
SKU: AB194690
Price: 4769 CNY
Availability: InStock

Anti-Histone H3 (di methyl K27) antibody - ChIP Grade

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(5 Publications)

Rabbit Polyclonal H3 di methyl K27 antibody. Suitable for ChIP, Dot, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat, Synthetic peptide, Recombinant full length protein - Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human H3-4 di methyl K27.

查看别名

H3FT, HIST3H3, H3-4, Histone H3.1t, H3/t, H3t, H3/g, Histone H3.4, H3K27me2, H3K27me, H3K27

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Paraffin-embedded human kidney cancer tissue stained for Histone H3 (di methyl K27) using ab194690 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Immunofluorescence staining of 293T cells stained for Histone H3 (di methyl K27) with ab194690. Nuclei are labeled with DAPI (Blue).

ChIP - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Chromatin immunoprecipitation analysis of extracts of 293T cell line, using ab194690 and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Paraffin-embedded rat brain tissue stained for Histone H3 (di methyl K27) using ab194690 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Paraffin-embedded mouse heart tissue stained for Histone H3 (di methyl K27) using ab194690 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (ab194690)

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

Histone H3 protein expressed in E.coli at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 15 kDa

false

Supplier Data

Dot Blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Dot-blot analysis of methylation peptides using ab194690 at 1 : 1000 dilution.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

ChIP, WB, IHC-P, ICC/IF, Dot

applications

免疫原

Synthetic Peptide within Human H3-4 di methyl K27. The exact immunogen used to generate this antibody is proprietary information.

Q16695

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1/20 - 1/100", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3-4 di methyl K27

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37298094

2023

Epigenetic Histone Methylation of PPARγ and CPT1A Signaling Contributes to Betahistine Preventing Olanzapine-Induced Dyslipidemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqing Su,Chao Deng,Xuemei Liu,Jiamei Lian

FEBS letters 593:2556-2565 PubMed31254363

2019

Protective effect of histone methyltransferase NSD3 on ISO-induced cardiac hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Chen,Dongdong Jian,Linwei Zhao,Xiaobiao Zang,Weifeng Song,Jifang Ma,Zhen Jia,Xianqing Wang,Chuanyu Gao

Clinical epigenetics 9:95 PubMed28878842

2017

Efficacy of EZH2 inhibitory drugs in human papillomavirus-positive and human papillomavirus-negative oropharyngeal squamous cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Cameron D Lindsay,Morris A Kostiuk,Jeff Harris,Daniel A O'Connell,Hadi Seikaly,Vincent L Biron

Cell stem cell 20:274-289.e7 PubMed27939217

2016

PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.

Applications

Species

Human

Zhexin Zhu,Chunliang Li,Yanwu Zeng,Jianyi Ding,Zepeng Qu,Junjie Gu,Laixiang Ge,Fan Tang,Xin Huang,Chenlin Zhou,Ping Wang,Deyou Zheng,Ying Jin

Molecular medicine reports 12:7561-7 PubMed26459745

2015

Epigenetic modifications of histone H3 during the transdifferentiation of Thy-1(+) Lin(‑) bone marrow cells into hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Liao,Yixin Liao,Yantai Zou,Guanhong Li,Caixian Liao

View all publications

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Anti-Histone H3 (di methyl K27)抗体- ChIP Grade

Anti-Histone H3 (di methyl K27) antibody - ChIP Grade

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

ChIP - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Dot Blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)