AB10799

Anti-Histone H3 抗体 [mAbcam 10799] - ChIP Grade

Name: Histone H3抗体[mAbcam 10799] - Nuclear Marker and ChIP Grade (ab10799)| Abcam中文官网
Brand: Abcam Limited
SKU: AB10799
Price: 1594 CNY
Availability: InStock
Rating: 4 (17 reviews)

Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade

BOND RX™ Validated
了解详情

(17 Reviews)

(179 Publications)

Anti-Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799) is a mouse monoclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ChIP. Suitable for Cow, Drosophila melanogaster, Human, Mouse, Rat,.

- Over 140 publications
- Trusted since 2005

查看别名

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

7 Images

Supplier Data

Western blot - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (AB10799)

All lanes:

Western blot - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (ab10799) at 5 µg/mL

Lane 1:

Histone H2A Recombinant Protein at 0.1 µg/mL

Lane 2:

Histone H2B Recombinant Protein at 0.1 µg/mL

Lane 3:

Histone H3.1 Recombinant Protein at 0.1 µg/mL

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg/mL

Lane 5:

HeLa nuclear lysate (triton enriched) at 10 µg/mL

Lane 6:

NIH 3T3 nuclear lysate (triton enriched) at 10 µg/mL

Lane 7:

PC12 nuclear lysate (triton enriched) at 10 µg/mL

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

false

Exposure time: 20s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (AB10799)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (AB10799)

ab10799 staining human kidney sections by IHC-P using EXPOSE IHC detection kit (ab80436). Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab10799, 5μg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (AB10799)

IHC image of Histone H3 staining in human breast carcinoma FFPE section, performed on a Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10799, 1μg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Lab

Immunoprecipitation - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (AB10799)

Histone H3 [mAbcam 10799] - ChIP Grade was immunoprecipitated using 0.5mg HeLa whole cell extract, 5μg of Mouse monoclonal to and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).

The antibody was incubated under agitation with Protein G beads for 10min, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10799.

Secondary : Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

Band : 15kDa; [mAbcam 10799] Histone H3 - ChIP Grade.

All lanes:

Immunoprecipitation - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (ab10799)

Predicted band size: 15 kDa

true

Exposure time: 20min

ChIP - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (AB10799)

ChIP

Supplier Data

ChIP - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (AB10799)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab10799 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Project

Western blot - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (AB10799)

All lanes:

Western blot - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (ab10799) at 1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

Calf Thymus Histone preparation nuclear lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 90s

Lab

Western blot - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (AB10799)

We recommend using 3% milk as the blocking agent in Western Blot.

All lanes:

Western blot - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade (ab10799) at 5 µg/mL

Lane 1:

Histone H1 Recombinant Protein at 0.1 µg

Lane 2:

Histone H2A Recombinant Protein at 0.1 µg

Lane 3:

Histone H2B Recombinant Protein at 0.1 µg

Lane 4:

Histone H3.1 Recombinant Protein at 0.1 µg

Lane 5:

Histone H4 Recombinant Protein at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

true

Exposure time: 4min

不同偶联物与剂型 (1)

Carrier free

Anti-Histone H3 antibody [mAbcam 10799] - ChIP Grade - BSA and Azide free

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

mAbcam 10799

亚型

IgG3

轻链类型

kappa

不含载体蛋白

反应种属

Mouse, Rat, Cow, Human, Drosophila melanogaster

应用

IP, ChIP, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-4 µg for 25 µg chromatin", "ChIP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1 µg/mL", "IP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Drosophila melanogaster": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "2-4 µg for 25 µg chromatin", "ChIP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1 µg/mL", "IP-species-notes": "" }, "Mammals": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Monkey": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant full length protein": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ChIP in Cow, Drosophila melanogaster, Human, Mouse, Rat, samples.

What is the molecular weight of Histone H3?
Anti-Histone H3 [mAbcam 10799] - ChIP Grade (ab10799) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa.

Trusted by the scientific community
Anti-Histone H3 [mAbcam 10799] - ChIP Grade (ab10799) was first used in a scientific publication in 2005 and has been cited over 140 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H3 [mAbcam 10799] - ChIP Grade (ab10799) has over 15 independent reviews from customers.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式

Liquid

纯度

IgG fraction

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1

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文献 (179)

Recent publications for all applications. Explore the full list and refine your search

NAR cancer 7:zcaf023 PubMed40809945

2025

SETD6 mediates selective interaction and genomic occupancy of BRD4 and MITF in melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tzofit Elbaz Biton,Michal Feldman,Tomer Davidy,Nili Tickotsky Moskovitz,Liron Levin,Daniel Sevilla,Colin R Goding,Emily Bernstein,Dan Levy

Cell death & disease 16:553 PubMed40701963

2025

H3K14 lactylation exacerbates neuronal ferroptosis by inhibiting calcium efflux following intracerebral hemorrhagic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Sun,Jian-Nan Zhang,Ting Lan,Lei Shi,Liye Hu,Liyan Yan,Chao Wei,Lisha Hei,Weihua Wu,Zhaoli Luo,Meng Liu,Xingmei Ren,Yamei Wang,Yabin Lu,Peipei Wang,Chenguang Zhang,Liheng Bian,Xingquan Zhao,Fei Yang,Qian Li

Nature communications 16:5000 PubMed40442089

2025

Cell-context response to germ layer differentiation signals is predetermined by the epigenome in regionalized epiblast populations.

Applications

Unspecified application

Species

Unspecified reactive species

Niels Alvaro Menezes,Kathryn Johanna Peterson,Xiaogang Guo,Veronica Castiglioni,Adrija Kalvisa,Katarzyna Filimonow,Karen Schachter,Christina Maria Schuh,Athanasios Pasias,Luca Mariani,Joshua Mark Brickman,Jakub Sedzinski,Elisabetta Ferretti

iScience 28:111953 PubMed40083716

2025

KAT6B overexpression in mice causes aggression, anxiety, and epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Maria I Bergamasco,Ezgi Ozturk,Pablo M Casillas-Espinosa,Alexandra L Garnham,Waruni Abeysekera,Verena C Wimmer,Pradeep Rajasekhar,Hannah K Vanyai,Lachlan Whitehead,Marnie E Blewitt,Kelly Rogers,Adam P Vogel,Anthony J Hannan,Gordon K Smyth,Nigel C Jones,Tim Thomas,Anne K Voss

Science advances 11:eadr1453 PubMed39970210

2025

Disabling leading and lagging strand histone transmission results in parental histones loss and reduced cell plasticity and viability.

Applications

Unspecified application

Species

Unspecified reactive species

Leonie Kollenstart,Alva Biran,Nicolas Alcaraz,Nazaret Reverón-Gómez,Victor Solis-Mezarino,Moritz Völker-Albert,Fion Jenkinson,Valentin Flury,Anja Groth

Nature communications 16:1675 PubMed39955279

2025

SRBD1 facilitates chromosome segregation by promoting topoisomerase IIα localization to mitotic chromosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Courtney A Lovejoy,Sarah R Wessel,Rahul Bhowmick,Yuki Hatoyama,Masato T Kanemaki,Runxiang Zhao,David Cortez

Cell communication and signaling : CCS 23:64 PubMed39910535

2025

Extracellular vimentin is a damage-associated molecular pattern protein serving as an agonist of TLR4 in human neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Łukasz Suprewicz,Krzysztof Fiedoruk,Karol Skłodowski,Evan Hutt,Magdalena Zakrzewska,Alicja Walewska,Piotr Deptuła,Agata Lesiak,Sławomir Okła,Peter A Galie,Alison E Patteson,Paul A Janmey,Robert Bucki

Nature 637:974-982 PubMed39779849

2025

Bidirectional histone monoaminylation dynamics regulate neural rhythmicity.

Applications

Unspecified application

Species

Unspecified reactive species

Qingfei Zheng,Benjamin H Weekley,David A Vinson,Shuai Zhao,Ryan M Bastle,Robert E Thompson,Stephanie Stransky,Aarthi Ramakrishnan,Ashley M Cunningham,Sohini Dutta,Jennifer C Chan,Giuseppina Di Salvo,Min Chen,Nan Zhang,Jinghua Wu,Sasha L Fulton,Lingchun Kong,Haifeng Wang,Baichao Zhang,Lauren Vostal,Akhil Upad,Lauren Dierdorff,Li Shen,Henrik Molina,Simone Sidoli,Tom W Muir,Haitao Li,Yael David,Ian Maze

Cancer & metabolism 12:39 PubMed39716277

2024

Similar deficiencies, different outcomes: succinate dehydrogenase loss in adrenal medulla vs. fibroblast cell culture models of paraganglioma.

Applications

Unspecified application

Species

Unspecified reactive species

Fatimah J Al Khazal,Sanjana Mahadev Bhat,Yuxiang Zhu,Cristina M de Araujo Correia,Sherry X Zhou,Brandon A Wilbanks,Clifford D Folmes,Gary C Sieck,Judith Favier,L James Maher

Clinical and translational medicine 14:e70128 PubMed39658533

2024

Epithelial NSD2 maintains FMO-mediated taurine biosynthesis to prevent intestinal barrier disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Xu,Xiuying Xiao,Chunxiao Ma,Ziyi Wang,Wenxin Feng,Hanyu Rao,Wei Zhang,Ningyuan Liu,Rebiguli Aji,Xiangjun Meng,Wei-Qiang Gao,Li Li

View all publications

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Anti-Histone H3 抗体 [mAbcam 10799] - ChIP Grade