AB272151

Anti-Histone H3 (acetyl K9 + K14)抗体

Name: Histone H3 (acetyl K9 + K14)抗体 (ab272151)| Abcam中文官网
Brand: Abcam Limited
SKU: AB272151
Price: 5847 CNY
Availability: InStock

Anti-Histone H3 (acetyl K9 + K14) antibody

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Rabbit Polyclonal H3 acetyl K9 + K14 antibody. Suitable for Dot, WB, ICC/IF and reacts with Mouse, Caenorhabditis elegans, Human samples. Immunogen corresponding to Synthetic Peptide within Human H3C1 acetyl K9 + K14.

查看别名

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9 + K14) antibody (AB272151)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9 + K14) antibody (AB272151)

Immunofluorescence analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (acetyl K9 + K14) (green) with ab272151 at 5 μg/ml overnight at 2-8°C, followed by Goat Anti-Rabbit IgG DyLight™488 at 5 μg/ml for 1 hour at room temperature.

Nuclear Counterstain : DAPI. Actin Filament Stain : Texas Red - X Phallodin.

Staining : (A) DAPI. (B) ab272151 + DyLight™488. (C) Actin-X Phallodin. (D) Merge A+B. (E) Merge A+B+C. (F) Secondary Only Merge.

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9 + K14) antibody (AB272151)

Immunofluorescence analysis of 0.5% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (acetyl K9 + K14) (green) with ab272151 at 1/200 dilution for 1 hour at room temperature, followed by FITC secondary antibody at 1/10000 for 45 minutes at room temperature. Nuclei and actin are counterstained with Dapi (blue) and Phalloidin (red).

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Western blot - Anti-Histone H3 (acetyl K9 + K14) antibody (AB272151)

All lanes:

Western blot - Anti-Histone H3 (acetyl K9 + K14) antibody (ab272151) at 1/500 dilution

All lanes:

C.elegans embryo lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

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Western blot - Anti-Histone H3 (acetyl K9 + K14) antibody (AB272151)

All lanes:

Western blot - Anti-Histone H3 (acetyl K9 + K14) antibody (ab272151) at 1/500 dilution

All lanes:

NIH/3T3 (mouse embryo fibroblast cell line) histone preps at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Unknown

Dot Blot - Anti-Histone H3 (acetyl K9 + K14) antibody (AB272151)

Dot Blot of ab272151.

Lane 1 : K9-K14.

Lane 2 : K9-KAc-K14-Kac.

Lane 3 : K9-K14-Kac.

Lane 4 : K9-Ac-K14.

Load : 0.3, 1, and 3 μg of peptide. Primary antibody ab272151 for 45 min at 4°C. HRP rabbit secondary antibody at 1/10000 for 45 minutes at room temperature.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human, Caenorhabditis elegans

应用

ICC/IF, WB, Dot

applications

免疫原

Synthetic Peptide within Human H3C1 acetyl K9 + K14. The exact immunogen used to generate this antibody is proprietary information.

P68431

特异性

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "" }, "Mouse": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1 µg/mL", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Anti-Histone H3 [ac Lys9, ac Lys14] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

存储溶液

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 acetyl K9 + K14

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (acetyl K9 + K14)抗体

Anti-Histone H3 (acetyl K9 + K14) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9 + K14) antibody (AB272151)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9 + K14) antibody (AB272151)

Western blot - Anti-Histone H3 (acetyl K9 + K14) antibody (AB272151)

Western blot - Anti-Histone H3 (acetyl K9 + K14) antibody (AB272151)

Dot Blot - Anti-Histone H3 (acetyl K9 + K14) antibody (AB272151)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性