Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-Histone H3 (acetyl K4)抗体[EPR16596] - ChIP Grade (ab176799)

Reviews (1) Submit a question References (19)
Dot Blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
  • Peptide Array - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
  • ChIP-sequencing - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
  • ChIP - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
  • Dot Blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
  • Western blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
  • Western blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
  • Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16596] to Histone H3 (acetyl K4) - ChIP Grade
  • Suitable for: WB, ICC/IF, Dot blot, ChIP, PepArr, ChIP-sequencing
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Histone H3 (acetyl K4)抗体[EPR16596] - ChIP Grade
    参阅全部 Histone H3 一抗

  • 描述

    兔单克隆抗体[EPR16596] to Histone H3 (acetyl K4) - ChIP Grade

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IF, Dot blot, ChIP, PepArr, ChIP-sequencingmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa treated with 500 ng/mlTrichostatin A for 4 hours whole cell lysates; NIH/3T3 treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates. ICC/IF: HeLa cells. ChIP: Chromatin prepared from HeLa cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab176799于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
ICC/IF
1/1000.
Dot blot
1/1000.
ChIP (1)
Use 2 µg for 25 µg of chromatin.
PepArr
Use a concentration of 0.1 µg/ml.
ChIP-sequencing
Use at an assay dependent concentration.
说明
WB
1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
ICC/IF
1/1000.
Dot blot
1/1000.
ChIP
Use 2 µg for 25 µg of chromatin.
PepArr
Use a concentration of 0.1 µg/ml.
ChIP-sequencing
Use at an assay dependent concentration.

靶标

  • 功能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

  • 序列相似性

    Belongs to the histone H3 family.

  • 发展阶段

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

  • 翻译后修饰

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.

  • 细胞定位

    Nucleus. Chromosome.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

图片

  • Dot Blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
    Dot Blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
  • Peptide Array - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
    Peptide Array - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)

    ab176799 (0.1μg/mL) was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). A Goat Anti-Rabbit IgG, (H+L), Fluo 647nm conjugated at 1/50000 was used as the secondary antibody. Protein Array Washing Buffer, 2*10 mins and 2*5 mins. Blocking buffer was 5% BSA in TBST and Innoscan 710 scanner was used.
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

  • ChIP-sequencing - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
    ChIP-sequencing - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 4 µg of ab176799 (EPR16596). ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. 

     

    Additional screenshots of mapped reads can be downloaded here. 

  • ChIP - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
    ChIP - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)

    Chromatin was prepared from HeLa (Human epithelial cells from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab176799 (red), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

  • Dot Blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
    Dot Blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)

    Dot blot analysis of Histone H3 (acetyl K4) labeled with ab176799 at 1/1000 dilution.

    Lane 1: Human H3(acetyl K4) peptide a
    Lane 2: Human H3(methyl K4) peptide c

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as secondary antobody at 1/100000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 180 minutes.

  • Western blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
    Western blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
    All lanes : Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799) at 1/10000 dilution

    Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/mlTrichostatin A for 4 hours whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
    Western blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
    All lanes : Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799) at 1/10000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, treated with Trichostatin A (500 ng/ml) for 4 hours or untreated, labeling Histone H3 (acetyl K4) with ab176799 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line. The expression increased after treatment with Trichostatin A (500 ng/ml) for 4 hours.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab176799 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

     

  • Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)
    Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade (ab176799)

文献 (19)

发表研究结果有使用 ab176799？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab176799 被引用在 19 文献中.

  • Al Madhoun A  et al. Adipose Tissue Caveolin-1 Upregulation in Obesity Involves TNF-α/NF-κB Mediated Signaling. Cells 12:N/A (2023). PubMed: 37048092
  • Xing X  et al. Fisetin Delays Postovulatory Oocyte Aging by Regulating Oxidative Stress and Mitochondrial Function through Sirt1 Pathway. Molecules 28:N/A (2023). PubMed: 37513404
  • Wu Y  et al. Plin2-mediated lipid droplet mobilization accelerates exit from pluripotency by lipidomic remodeling and histone acetylation. Cell Death Differ 29:2316-2331 (2022). PubMed: 35614132
  • Xu H  et al. The Fng3 ING protein regulates H3 acetylation and H4 deacetylation by interacting with two distinct histone-modifying complexes. New Phytol 235:2350-2364 (2022). PubMed: 35653584
  • Al Madhoun A  et al. Caveolin-1 rs1997623 Single Nucleotide Polymorphism Creates a New Binding Site for the Early B-Cell Factor 1 That Instigates Adipose Tissue CAV1 Protein Overexpression. Cells 11:N/A (2022). PubMed: 36497195
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

ChIP abreview for Anti-Histone H3 (acetyl K4) antibody [EPR16596] - ChIP Grade

 Good
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - nuclear (K562)
Specification
K562
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Andrew Perez

Verified customer

提交于 Mar 24 2022

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com