重组Anti-Histone H2B (testis specific)抗体[EPR12881] (ab178426)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12881] to Histone H2B (testis specific)
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Histone H2B (testis specific)抗体[EPR12881]
参阅全部 Histone H2B (testis specific) 一抗 -
描述
兔单克隆抗体[EPR12881] to Histone H2B (testis specific) -
宿主
Rabbit -
特异性
The immunogen used for this product shared more than 90% homology with many Histone H2B family members. Cross-reactivity with these proteins have not been confirmed experimentally.
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经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Human
不与反应: Rat -
免疫原
Recombinant fragment within Human Histone H2B (testis specific) aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q96A08 -
阳性对照
- WB: Human and mouse testis lysates. IHC-P: Human testis tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12881 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178426于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
靶标
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功能
Variant histone specifically required to direct the transformation of dissociating nucleosomes to protamine in male germ cells. Entirely replaces classical histone H2B prior nucleosome to protamine transition and probably acts as a nucleosome dissociating factor that creates a more dynamic chromatin, facilitating the large-scale exchange of histones. Also expressed maternally and is present in the female pronucleus, suggesting a similar role in protamine replacement by nucleosomes at fertilization (By similarity). Also found in fat cells, its function and the presence of post-translational modifications specific to such cells are still unclear. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
组织特异性
Mainly expressed in testis, and the corresponding protein is also present in mature sperm (at protein level). Also found in some fat cells. -
序列相似性
Belongs to the histone H2B family. -
翻译后修饰
Monoubiquitination at Lys-36 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-122 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.
Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
Acetylated during spermatogenesis. Acetylated form is most abundant in spermatogonia compared to spermatocytes and round spermatids.
Phosphorylated at Thr-117 in spermatogonia, spermatocytes and round spermatids.
Methylated at Lys-118 in spermatogonia, spermatocytes and round spermatids. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 255626 Human
- Entrez Gene: 319177 Mouse
- Omim: 609904 Human
- SwissProt: Q96A08 Human
- SwissProt: P70696 Mouse
- Unigene: 591786 Human
- Unigene: 377879 Mouse
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别名
- HIST1H2BA antibody
- testis antibody
- bA317E16.3 antibody
see all
图片
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All lanes : Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426) at 1/20000 dilution
Lane 1 : Histone H2B1A (Q96A08) recombinant protein fragment (GST-Tag): aa2-101
Lane 2 : Histone H2B1B (P33778) recombinant protein fragment (GST-Tag): aa2-126
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
All lanes : Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Mouse testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 14 kDa -
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Histone H2B (testis specific) with ab178426 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from Human testis lysate immunoprecipitated using ab178426 at 1/10 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab178426 被引用在 1 文献中.
- Panner Selvam MK et al. Treatment of semen samples with a-chymotrypsin alters the expression pattern of sperm functional proteins-a pilot study. Andrology 6:345-350 (2018). PubMed: 29409138