AB177779

重组Anti-Histone H2B (mono methyl R79)抗体[EPR17612]

Anti-Histone H2B (mono methyl R79) antibody [EPR17612]

RabMAb
Advanced Validation
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal H2B mono methyl R79 antibody. Suitable for WB, PepArr and reacts with Mouse, Human samples. Cited in 1 publication.

查看别名

H2BFQ, HIST2H2BE, H2BC21, Histone H2B type 2-E, H2B-clustered histone 21, Histone H2B-GL105, Histone H2B.q, H2B/q, H2BR79me, H2BR79me1, H2BR79

3 Images

Supplier Data

Western blot - Anti-Histone H2B (mono methyl R79) antibody [EPR17612] (AB177779)

Blocking/Dilution buffer : 5% BSA /TBST.

All lanes:

Western blot - Anti-Histone H2B (mono methyl R79) antibody [EPR17612] (ab177779) at 1/50000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-Histone H2B (mono methyl R79) antibody [EPR17612] (AB177779)

Blocking/Dilution buffer : 5% BSA /TBST.

All lanes:

Western blot - Anti-Histone H2B (mono methyl R79) antibody [EPR17612] (ab177779) at 1/50000 dilution

All lanes:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Exposure time: 1min

Peptide Array - Anti-Histone H2B (mono methyl R79) antibody [EPR17612] (AB177779)

PepArr

Supplier Data

Peptide Array - Anti-Histone H2B (mono methyl R79) antibody [EPR17612] (AB177779)

ab177779 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17612

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, PepArr

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000", "WB-species-notes": "", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

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靶点信息

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

See full target information H2BC21 mono methyl R79

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 46:5809-5821 PubMed29931370

2018

Long noncoding RNA OCC-1 suppresses cell growth through destabilizing HuR protein in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Lan,Xuewei Xiao,Zhengchi He,Yu Luo,Chuanfang Wu,Ling Li,Xu Song

View all publications

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peptideArrayWebsite

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Histone H2B (mono methyl R79)抗体[EPR17612]

Anti-Histone H2B (mono methyl R79) antibody [EPR17612]

Western blot - Anti-Histone H2B (mono methyl R79) antibody [EPR17612] (AB177779)

Western blot - Anti-Histone H2B (mono methyl R79) antibody [EPR17612] (AB177779)

Peptide Array - Anti-Histone H2B (mono methyl R79) antibody [EPR17612] (AB177779)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原