重组Anti-Histone H2B (formyl K108)抗体[EPR17697] (ab177784)
![Western blot - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)](https://www.abcam.cn/ps/products/177/ab177784/Images/ab177784-247054-anti-histone-h2b-formyl-k108-antibody-epr17697-western-blot.jpg "Western blot - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17697] to Histone H2B (formyl K108)
- Suitable for: PepArr, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Histone H2B (formyl K108)抗体[EPR17697]
参阅全部 Histone H2B 一抗 -
描述
兔单克隆抗体[EPR17697] to Histone H2B (formyl K108) -
宿主
Rabbit -
经测试应用
适用于: PepArr, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa and NIH/3T3 cell lysates. IHC-P: Human colon, mouse liver and rat colon tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17697 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177784于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| PepArr |
Use at an assay dependent concentration.
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| WB |
1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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| IHC-P |
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
PepArr
Use at an assay dependent concentration. |
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WB
1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
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IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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相关性
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 337874 Human
- Entrez Gene: 54145 Human
- Entrez Gene: 8349 Human
- Entrez Gene: 104021 Mouse
- Entrez Gene: 64647 Rat
- SwissProt: O60814 Human
- SwissProt: P06899 Human
- SwissProt: P23527 Human
see all -
别名
- GL105 antibody
- H2B GL105 antibody
- H2B histone family member O antibody
see all
图片
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Western blot - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784) at 1/2000 dilution + Hela (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)](https://www.abcam.cn/ps/products/177/ab177784/Images/ab177784-247051-anti-histone-h2b-formyl-k108-antibody-epr17697-western-blot.png)
Western blot - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784) at 1/2000 dilution + NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Peptide Array - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)](https://www.abcam.cn/ps/products/177/ab177784/Images/ab177784-261505-anti-histone-h2b-formyl-k108-antibody-epr17697-peptide-array.jpg)
Peptide Array - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)ab177784 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)](https://www.abcam.cn/ps/products/177/ab177784/Images/ab177784-247049-anti-histone-h2b-formyl-k108-antibody-epr17697-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H2B (formyl K108) with ab177784 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)](https://www.abcam.cn/ps/products/177/ab177784/Images/ab177784-247047-anti-histone-h2b-formyl-k108-antibody-epr17697-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Histone H2B (formyl K108) with ab177784 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on mouse liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)](https://www.abcam.cn/ps/products/177/ab177784/Images/ab177784-247046-anti-histone-h2b-formyl-k108-antibody-epr17697-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H2B (formyl K108) with ab177784 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on rat colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)](https://www.abcam.cn/ps/products/177/ab177784/Images/ab177784-7-benefits-of-recombinant-antibodies.png)
Anti-Histone H2B (formyl K108) antibody [EPR17697] (ab177784)
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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