重组Anti-Histone H2B抗体[EP957Y] (ab52599)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (ab52599)](https://www.abcam.cn/ps/products/52/ab52599/Images/ab52599-6-Anti-Histone-H2B-antibody-EP957Y-Immunohistochemistry-bone-marrow-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (ab52599)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP957Y] to Histone H2B
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Histone H2B抗体[EP957Y]
参阅全部 Histone H2B 一抗 -
描述
兔单克隆抗体[EP957Y] to Histone H2B -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: A431 cell lysate, HeLa cell lysate; NIH/3T3 cell lysate; C6 cell lysate. Flow Cyt (intra): HeLa cells. IHC-P: Human breast carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP957Y -
同种型
IgG
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52599于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (3) |
1/20000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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| IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/20000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 337874 Human
- Entrez Gene: 54145 Human
- Entrez Gene: 8349 Human
- Entrez Gene: 104021 Mouse
- Entrez Gene: 64647 Rat
- SwissProt: P62808 Cow
- SwissProt: O60814 Human
- SwissProt: P06899 Human
see all -
别名
- GL105 antibody
- H2B GL105 antibody
- H2B histone family member O antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (ab52599)This image is courtesy of an Abreview submitted by Natalie PapazianImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of paraformaldehyde-fixed human bone marrow tissue staining with ab52599 at 1/200 dilution. Secondary antibody was Alexa Fluor™ 555 Donkey anti-Rabbit IgG (H+L) at 1/200 dilution. Samples were incubated with the primary antibody with PBS for 1 hour. Blocking was done using 5% serum for 1 hour at room temperature. Heat mediated antigen retrieval with 1mM EDTA PH8.0.
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![Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599)](https://www.abcam.cn/ps/products/52/ab52599/Images/ab52599-511297-anti-histone-h2b-antibody-ep957y-western-blot-tissue-lysate-human-mouse-rat.jpg)
Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599)All lanes : Anti-Histone H2B antibody [EP957Y] (ab52599) at 1/5000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599)](https://www.abcam.cn/ps/products/52/ab52599/Images/ab52599-64922-anti-histone-h2b-antibody-ep957y-western-blot.jpg)
Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599)Anti-Histone H2B antibody [EP957Y] (ab52599) at 1/20000 dilution + A431 cell lysate at 10 µg
Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa -
![Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] (ab52599)](https://www.abcam.cn/ps/products/52/ab52599/Images/ab52599-195345-anti-histone-h2b-antibody-ep957y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] (ab52599)Overlay histogram showing HeLa cells stained with ab52599 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52599, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (ab52599)](https://www.abcam.cn/ps/products/52/ab52599/Images/ab52599-64921-anti-histone-h2b-antibody-ep957y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (ab52599)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab52599 at a dilution of 1/250-1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Histone H2B antibody [EP957Y] (ab52599)](https://www.abcam.cn/ps/products/52/ab52599/Images/ab52599-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H2B antibody [EP957Y] (ab52599)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab52599 被引用在 24 文献中.
- Wang X et al. Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation. Cell Res 31:664-683 (2021). PubMed: 33432115
- Jeong YM et al. 3D-Printed Collagen Scaffolds Promote Maintenance of Cryopreserved Patients-Derived Melanoma Explants. Cells 10:N/A (2021). PubMed: 33800001
- Jing C et al. Antibodies Against Pseudomonas aeruginosa Alkaline Protease Directly Enhance Disruption of Neutrophil Extracellular Traps Mediated by This Enzyme. Front Immunol 12:654649 (2021). PubMed: 33868297
- Jin Y et al. Long noncoding RNA PM maintains cerebellar synaptic integrity and Cbln1 activation via Pax6/Mll1-mediated H3K4me3. PLoS Biol 19:e3001297 (2021). PubMed: 34111112
- Tang M et al. circRNA-DURSA regulates trophoblast apoptosis via miR-760-HIST1H2BE axis in unexplained recurrent spontaneous abortion. Mol Ther Nucleic Acids 26:1433-1445 (2021). PubMed: 34938599