AB52599

重组Anti-Histone H2B抗体[EP957Y]

Name: Histone H2B抗体[EP957Y] (ab52599)| Abcam中文官网
Brand: Abcam Limited
SKU: AB52599
Price: 2545 CNY
Availability: InStock
Rating: 4 (4 reviews)

Anti-Histone H2B antibody [EP957Y]

RabMAb
Recombinant
了解详情

(4 Reviews)

(29 Publications)

Rabbit Recombinant Monoclonal H2B antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 29 publications.

查看别名

H2BFL, HIST1H2BC, H2BC6, H2BFH, HIST1H2BE, H2BC7, H2BFG, HIST1H2BF, H2BC8, H2BFA, HIST1H2BG, H2BC10, H2BFK, HIST1H2BI, H2BC4, Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, Histone H2B.g, Histone H2B.h, Histone H2B.k, Histone H2B.l, H2B/a, H2B/g, H2B/h, H2B/k, H2B/l

5 Images

Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] (AB52599)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] (AB52599)

Overlay histogram showing HeLa cells stained with ab52599 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52599, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (AB52599)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (AB52599)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab52599 at a dilution of 1/250-1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

AbReview80275****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (AB52599)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of paraformaldehyde-fixed human bone marrow tissue staining with ab52599 at 1/200 dilution. Secondary antibody was Alexa Fluor™ 555 Donkey anti-Rabbit IgG (H+L) at 1/200 dilution. Samples were incubated with the primary antibody with PBS for 1 hour. Blocking was done using 5% serum for 1 hour at room temperature. Heat mediated antigen retrieval with 1mM EDTA PH8.0.

This image is courtesy of an Abreview submitted by Natalie Papazian

Lab

Western blot - Anti-Histone H2B antibody [EP957Y] (AB52599)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599) at 1/5000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Exposure time: 180s

Unknown

Western blot - Anti-Histone H2B antibody [EP957Y] (AB52599)

All lanes:

Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599) at 1/20000 dilution

All lanes:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

不同偶联物与剂型 (2)

Biotin

Biotin Anti-Histone H2B antibody [EP957Y]
Carrier free

Anti-Histone H2B antibody [EP957Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP957Y

亚型

IgG

不含载体蛋白

反应种属

Rat, Human, Mouse

应用

WB, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

See full target information H2BC4

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文献 (29)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 15:1525789 PubMed40166371

2025

Screening the receptors for P35 lipoprotein and characterization of its functional binding domains.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Li,Xiaoliu Wang,Youyuan Ye,Zhuo Zeng,Li Chen,Kailan Peng,Hua Xiao,Siqi Gao,Haodang Luo,Yanhua Zeng

The Journal of biological chemistry 301:108305 PubMed39947471

2025

Integrin beta 1 and fibronectin mediate extracellular vesicle uptake and functional RNA delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Omnia M Elsharkasy,Willemijn S de Voogt,Maria Laura Tognoli,Leanne van der Werff,Jerney J Gitz-Francois,Cornelis W Seinen,Raymond M Schiffelers,Olivier G de Jong,Pieter Vader

Journal of experimental & clinical cancer research : CR 43:319 PubMed39648199

2024

Cryoablation-induced neutrophil Ca elevation and NET formation exacerbate immune escape in colorectal cancer liver metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongtong Tan,Yiquan Jiang,Lujun Shen,Gulijiayina Nuerhashi,Chunyong Wen,Ling Gu,Yujia Wang,Han Qi,Fei Cao,Tao Huang,Ying Liu,Weining Xie,Wuguo Deng,Weijun Fan

Pharmaceutical biology 62:285-295 PubMed38516898

2024

Diosgenin protects against cationic bovine serum albumin-induced membranous glomerulonephritis by attenuating oxidative stress and renal inflammation via the NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyan Jia,Ruihua Si,Guangzhen Liu,Qiming Zhong

Molecular cell 83:3049-3063.e6 PubMed37591243

2023

Cohesin mediates DNA loop extrusion and sister chromatid cohesion by distinct mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Kota Nagasaka,Iain F Davidson,Roman R Stocsits,Wen Tang,Gordana Wutz,Paul Batty,Melanie Panarotto,Gabriele Litos,Alexander Schleiffer,Daniel W Gerlich,Jan-Michael Peters

Frontiers in immunology 12:779667 PubMed35046941

2022

Modulation of IL-6 Expression by KLF4-Mediated Transactivation and PCAF-Mediated Acetylation in Sublytic C5b-9-Induced Rat Glomerular Mesangial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Xia,Yu Liu,Zhiwei Zhang,Yajuan Gong,Tianyi Yu,Dan Zhao,Wen Qiu,Yingwei Wang,Jing Zhang

Molecular therapy. Nucleic acids 26:1433-1445 PubMed34938599

2021

circRNA-DURSA regulates trophoblast apoptosis via miR-760-HIST1H2BE axis in unexplained recurrent spontaneous abortion.

Applications

Unspecified application

Species

Unspecified reactive species

Minyue Tang,Long Bai,Zhe Wan,Shan Wan,Yu Xiang,Yeqing Qian,Long Cui,Jiali You,Xiaoling Hu,Fan Qu,Yimin Zhu

PLoS biology 19:e3001297 PubMed34111112

2021

Long noncoding RNA PM maintains cerebellar synaptic integrity and Cbln1 activation via Pax6/Mll1-mediated H3K4me3.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Jin,Bowen Zhang,Junxia Lu,Yingdong Song,Wei Wang,Wei Zhang,Fanghong Shao,Meng Gong,Meiting Wang,Xiaolin Liang,Shuqin Li,Zhi Zhang,Ge Shan,Xiangting Wang

Frontiers in immunology 12:654649 PubMed33868297

2021

Antibodies Against Alkaline Protease Directly Enhance Disruption of Neutrophil Extracellular Traps Mediated by This Enzyme.

Applications

Unspecified application

Species

Unspecified reactive species

Chendi Jing,Chenghua Liu,Yu Liu,Ruli Feng,Run Cao,Zhangchun Guan,Bo Xuan,Yaping Gao,Qi Wang,Nana Yang,Yuanfang Ma,Lefu Lan,Jiannan Feng,Beifen Shen,Hui Wang,Yanyan Yu,Guang Yang

Cells 10: PubMed33800001

2021

3D-Printed Collagen Scaffolds Promote Maintenance of Cryopreserved Patients-Derived Melanoma Explants.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Mi Jeong,ChulHwan Bang,MinJi Park,Sun Shin,Seokhwan Yun,Chul Min Kim,GaHee Jeong,Yeun-Jun Chung,Won-Soo Yun,Ji Hyun Lee,Songwan Jin

View all publications

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