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AB271344

重组Anti-Histone H2B (acetyl K20)抗体[RM235]

Anti-Histone H2B (acetyl K20) antibody [RM235]

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Rabbit Recombinant Monoclonal H2B acetyl K20 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human H2BC1 acetyl K20.

查看别名

HIST1H2BA, TSH2B, H2BC1, Histone H2B type 1-A, Testis-specific histone H2B, TSH2B.1, hTSH2B, H2BK20ac

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K20) antibody [RM235] (AB271344)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K20) antibody [RM235] (AB271344)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H2B (acetyl K20) using ab271344 at 2 μg/ml (red). Actin filaments have been labeled with fluorescein phalloidin (green).``

ELISA - Anti-Histone H2B (acetyl K20) antibody [RM235] (AB271344)
  • ELISA

Supplier Data

ELISA - Anti-Histone H2B (acetyl K20) antibody [RM235] (AB271344)

ab271344 specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

Western blot - Anti-Histone H2B (acetyl K20) antibody [RM235] (AB271344)
  • WB

Supplier Data

Western blot - Anti-Histone H2B (acetyl K20) antibody [RM235] (AB271344)

All lanes:

Western blot - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344) at 0.5 µg/mL

Lane 1:

Acid extracts of HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with sodium butyrate

Lane 2:

Acid extracts of untreated HeLa cells

Predicted band size: 14 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

RM235

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ELISA, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human H2BC1 acetyl K20. The exact immunogen used to generate this antibody is proprietary information.

Q96A08

特异性

No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.2-1 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Variant histone specifically required to direct the transformation of dissociating nucleosomes to protamine in male germ cells (By similarity). Entirely replaces classical histone H2B prior nucleosome to protamine transition and probably acts as a nucleosome dissociating factor that creates a more dynamic chromatin, facilitating the large-scale exchange of histones (By similarity). Core component of nucleosome (By similarity). Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template (By similarity). Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability (By similarity). DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling (By similarity). Also found in fat cells, its function and the presence of post-translational modifications specific to such cells are still unclear (PubMed : 21249133).
See full target information H2BC1 acetyl K20
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